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A kind of Siniperca tlr3 gene and its application

A mandarin fish and base technology, which can be applied in application, genetic engineering, plant genetic improvement and other directions, can solve the problems of lack of cloning and functional research, and achieve the goal of promoting the process of genetic breeding, improving the economic benefits of mandarin fish farming and improving economic benefits. Effect

Active Publication Date: 2019-08-30
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of relevant research on the cloning and function of fish TLR3 gene.

Method used

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  • A kind of Siniperca tlr3 gene and its application
  • A kind of Siniperca tlr3 gene and its application
  • A kind of Siniperca tlr3 gene and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036] 1. Cloning of TLR3 cDNA of Siniperca sinensis

[0037] The primers used for TLR3 gene cDNA amplification are shown in Table 1 below:

[0038] Table 1 The sequences of primers used in TLR3 gene cDNA amplification

[0039] Primer name Primer sequence (5'-3') Purpose GSP-TLR3-1 GCAGGAGACCTTCTTC TLR3 5' GSP-TLR3-2 CGGTCCCGTCATAAAATAAC TLR3 5' GSP-TLR3-3 CGGGAGGAGAAGGGAACG TLR3 5' 3' CDS Primer A AAGCAGTGGTATCAACGCAGACTAC TLR3 3' GSP-TLR3-4 TATCTCGCTCACTCTTCCTCCGCAG TLR3 3' GSP-TLR3-5 GCCTGTCCATAAGGAGAGGGTGCC TLR3 3' AUAP GGCCACGCGTCGACTAGTAC Universal 5'-Race AAP GGCCACGCGTCGACTAGTAC(G)16 Universal 5'-Race UPM CTAATACGACTCACTATAGGGC Universal 3’-Race

[0040] 1. Amplification of the 5' end sequence of Siniperca sinensis TLR3

[0041] (1) Synthesis, purification and tailing of the first strand of cDNA

[0042] The extracted total RNA was synthesized with TLR3 first strand cDN...

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Abstract

The invention discloses Siniperca chuatsi TLR3 gene and application thereof. The Siniperca chuatsi TLR3 gene has cDNA nucleotide sequence shown as SEQ ID NO. 1, has gDNA nucleotide sequence shown as SEQ ID NO. 2, and an amino acid sequence shown as SEQ ID NO. 3. The Siniperca chuatsi TLR3 gene is applicable to early detection of pathogen affections in Siniperca chuatsi, providing a new idea for the early diagnosis of viral or bacterial infections in Siniperca chuatsi, benefiting the early prevention and treatment of fish diseases and improving economic benefit in Siniperca chuatsi farming. Inaddition, the Siniperca chuatsi TLR3 gene is also applicable to disease-resistant germplasm breeding of Siniperca chuatsi, providing a new idea for breeding of Siniperca chuatsi, benefiting the promotion of the genetic breeding process of Siniperca chuatsi, and improving economic benefit of Siniperca chuatsi farming.

Description

technical field [0001] The invention belongs to the fields of aquaculture and biotechnology, and in particular relates to a TLR3 gene of mandarin fish and its application in detection of mandarin mandarin fish at the initial stage of pathogen infection and breeding of disease-resistant germplasm of mandarin fish. Background technique [0002] Siniperca chuatsi, belonging to Perciformes, Sinipercinae, and Siniperca, is a valuable economic fish and an important freshwater aquaculture species in my country. However, with the continuous expansion of the scale of artificial breeding in recent years, the germplasm degradation and disease resistance of the mandarin fish are relatively common, leading to the increasingly rampant exposure of viral diseases, and there is no very effective control method so far. All cause serious economic losses every year, seriously restricting the sustainable development of its breeding industry. Therefore, the early detection and diagnosis of patho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/12C07K14/46C12Q1/6883C12Q1/6888
Inventor 黄鹤忠路瑶叶金明肖攀李泽金锐铭董同瑚
Owner SUZHOU UNIV
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