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Kit for detecting methylation degree of ptpn1 gene promoter region related to type 2 diabetes and application thereof

A technology of gene promoter region and type 2 diabetes is applied in the field of detection kits for auxiliary diagnosis of type 2 diabetes, which can solve the problems of non-disclosure and the like, and achieve the effects of strong pertinence and high detection efficiency

Active Publication Date: 2017-12-01
NINGBO UNIV
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Problems solved by technology

At present, there is no published information about the detection of type 2 diabetes at home and abroad. PTPN1 Relevant research reports on kits for the degree of methylation in gene promoter regions

Method used

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  • Kit for detecting methylation degree of ptpn1 gene promoter region related to type 2 diabetes and application thereof
  • Kit for detecting methylation degree of ptpn1 gene promoter region related to type 2 diabetes and application thereof
  • Kit for detecting methylation degree of ptpn1 gene promoter region related to type 2 diabetes and application thereof

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[0019] 1. Collection of research objects

[0020] Samples of patients with type 2 diabetes were collected from a chronic disease research institute in Shenzhen. The diagnostic criteria for type 2 diabetes were based on the 2010 American Diabetes Association (ADA) guidelines, excluding hypertension, coronary heart disease and other heart diseases, renal insufficiency, drug abuse, obesity or other After severe diseases and other diseases, 97 cases of T2D were finally determined as the case group (59.79±9.39 years old), and 97 normal people who matched age, sex, BMI and had no other diseases were collected as the control group (59.79±9.39 years old). Under the premise of informed consent, blood was drawn from all subjects to measure general biochemical indicators such as total cholesterol, triglycerides, low-density lipoprotein, urea and creatinine. At the same time, 3ml of venous blood was drawn into EDTA anticoagulant tubes and kept at -80°C. Stored for the unified extraction o...

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Abstract

The invention discloses a reagent kit capable of being used for detecting methylation degrees of PTPN1 genes related to type-2 diabetes and application of the reagent kit. The reagent kit is characterized by comprising a pair of PTPN1 gene methylation specific amplification primers and methylation specific sequencing primers; upstream primers are provided with nucleotide sequences shown as SEQ ID NO.1, downstream primers are provided with nucleotide sequences shown as SEQ ID NO.2, and the methylation specific sequencing primers are shown as SEQ ID NO.3. The reagent kit and the application have the advantages that the type-2 diabetes of female population can be conveniently and quickly detected at molecular level by the diagnosis reagent kit, the reagent kit is high in detection efficiency and pertinence, and medicine with target, namely, PTPN1 gene methylation, can hopefully become a novel means for assisting in diagnosing, detecting and screening the type-2 diabetes.

Description

technical field [0001] The invention relates to a detection kit for auxiliary diagnosis of type 2 diabetes, in particular to a detection kit which can be used for detection of type 2 diabetes PTPN1 A kit for the degree of methylation in the gene promoter region. Background technique [0002] Diabetes mellitus is a chronic metabolic disease that is caused by absolute or relative insufficiency of insulin in the blood, leading to hyperglycemia and glycosuria, which in turn causes fat and protein metabolism disorders. Clinically, polyuria, polydipsia, and polydipsia can occur , polyphagia, weight loss and other manifestations, severe cases are prone to acute complications such as ketoacidosis or chronic complications such as blood vessels and nerves. Diabetes is a common endocrine and metabolic disease, and its prevalence is gradually increasing with the improvement of living conditions and living standards. According to statistics, the number of diabetic patients in China is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/154
Inventor 韩丽媛段世伟麦一峰黄清周恩呈刘艳芬陆南佳
Owner NINGBO UNIV
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