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Kit for detecting the degree of methylation in the promoter region of the glud1 gene associated with type 2 diabetes and its application

A technology of gene promoter region and type 2 diabetes, applied in the direction of recombinant DNA technology, DNA/RNA fragments, etc., can solve the problem of non-disclosure, etc., and achieve the effect of strong pertinence and high detection efficiency

Active Publication Date: 2018-02-09
NINGBO UNIV
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Problems solved by technology

At present, there is no public information about the detection of type 2 diabetes at home and abroad. GLUD1 Relevant research reports on kits for the degree of methylation in gene promoter regions

Method used

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  • Kit for detecting the degree of methylation in the promoter region of the glud1 gene associated with type 2 diabetes and its application
  • Kit for detecting the degree of methylation in the promoter region of the glud1 gene associated with type 2 diabetes and its application
  • Kit for detecting the degree of methylation in the promoter region of the glud1 gene associated with type 2 diabetes and its application

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[0019] 1. Collection of research objects

[0020] Samples of patients with type 2 diabetes were collected from a chronic disease research institute in Shenzhen. The diagnostic criteria for type 2 diabetes were based on the 2010 American Diabetes Association (ADA) guidelines, excluding hypertension, coronary heart disease and other heart diseases, renal insufficiency, drug abuse, obesity or other After severe diseases and other diseases, 97 cases of T2D were finally determined as the case group (59.74±9.42 years old), and 97 normal people who matched age, sex, BMI and had no other diseases were collected as the control group (59.74±9.42 years old). Under the premise of informed consent, blood was drawn from all subjects to measure general biochemical indicators such as total cholesterol, triglycerides, low-density lipoprotein, urea and creatinine. At the same time, 3ml of venous blood was drawn into EDTA anticoagulant tubes and kept at -80°C. Stored for the unified extraction o...

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Abstract

The invention discloses a kit for detecting the degree of methylation of the GLUD1 gene related to type 2 diabetes and its application. The kit is characterized in that the kit includes a pair of GLUD1 gene methylation-specific amplification primers and Sequencing primers, wherein the upstream primer has the nucleotide sequence shown in SEQ ID NO.1, the downstream primer has the nucleotide sequence shown in SEQ ID NO.2, and the methylation-specific sequencing primer has the nucleotide sequence shown in SEQ ID NO. As shown in 3, the advantage is that the diagnostic kit can conveniently and quickly realize the detection of type 2 diabetes in female population at the molecular level, with high detection efficiency and strong pertinence. At the same time, drugs targeting GLUD1 gene methylation are expected It has become a new method for auxiliary diagnosis, detection and screening of type 2 diabetes.

Description

technical field [0001] The invention relates to a detection kit for auxiliary diagnosis of type 2 diabetes, in particular to a detection kit which can be used for detection of type 2 diabetes GLUD1 A kit for the degree of methylation in the gene promoter region. Background technique [0002] Diabetes mellitus is a chronic metabolic disease that is caused by absolute or relative insufficiency of insulin in the blood, leading to hyperglycemia and glycosuria, which in turn causes fat and protein metabolism disorders. Clinically, polyuria, polydipsia, and polydipsia can occur , polyphagia, weight loss and other manifestations, severe cases are prone to acute complications such as ketoacidosis or chronic complications such as blood vessels and nerves. Diabetes is a common endocrine and metabolic disease, and its prevalence is gradually increasing with the improvement of living conditions and living standards. According to statistics, the number of diabetic patients in China is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 段世伟韩丽媛麦一峰黄清周恩呈刘艳芬陆南佳
Owner NINGBO UNIV
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