Mountain Tai rhizoma polygonati micro rhizome rapid breeding method

A rhizome and Polygonatum rhizome technology, applied in the field of rapid propagation of microrhizome of Taishan Polygonatum, can solve the problems of unfavorable large-scale promotion, insufficiently stable survival rate, high operation requirements, etc., and achieve the advantages of shortening seedling time, reducing water loss, and strong environmental adaptability Effect

Inactive Publication Date: 2019-01-18
TAISHAN RES INST OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the propagation of Taishan Polygonatum tissue culture seedlings reported at present is mainly based on green seedling transplantation, and the transplanting process of hardened seedlings is easily affected by humidity and causes dead seedlings, which makes the survival rate of hardened seedlings transplanted not stable enough, and the operation requirements are high. It is conducive to large-scale promotion. Therefore, in combination with the field rhizome propagation method, explore the use of tissue culture to propagate the aseptic micro-rhizome of Taishan Polygonatum, directly transplant the aseptic micro-rhizome, omit the process of green seedling hardening and reduce the humidity requirements, which can avoid The loss of seedlings caused by transplanting green seedlings greatly reduces the operation process, and is conducive to long-distance transportation and large-scale promotion of planting. It can provide technical support for seedling breeding for the artificial large-scale promotion of Taishan Polygonatum, and open up a new era of seedling propagation. way
[0004] At present, the research on the hardening and transplanting technology of Taishan Polygonatum tissue culture seedlings is mainly based on the conventional transplanting technology of complete green seedlings with young tender roots, stems and leaves, while the use of tissue culture technology and field rhizome transplanting Combining technology to discuss the root propagation mode of Taishan Polygonatum microseed has not yet been published.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] From the established aseptic bottle seedlings of the Taishan Polygonatum high-frequency regeneration system, select a drop-shaped single bud that grows robustly and has a full terminal bud as the sterile body, with a diameter of 0.3-2 cm and the base downward, and inoculate it on the rooting Medium 1 / 2MS +NAA 0.4-1.0mg / L, after 20-30 days of culture, adventitious roots grow from the transverse stripes of the bud epidermis, the adventitious roots are white, the number is 1-4, the average surface is smooth and there are no fibrous roots, the length is 0.5-2 cm, this type of rooting bud can be directly used for transplanting, which is the microrhizome of Taishan Polygonatum.

[0034] Taishan Polygonatum high-frequency regeneration system aseptic bottle seedlings in the differentiation medium (the medium established by the authorized patented regeneration system, that is, MS+BA2.0-2.5 mg / L+IBA0.5-1.0 mg / L + sucrose 3- 5%.) After continuing to cultivate for 20-30 days, conti...

Embodiment 2

[0037] From the established aseptic bottle seedlings of Taishan Polygonatum high-frequency regeneration system, select a rhizome mass that grows vigorously and has at least one full terminal bud. This type of rhizome mass contains two main types, one is with one full terminal bud The water drop-shaped or long cylindrical buds, the second is the rhizome block without obvious terminal buds; the larger rhizome mass is cut into pieces with a diameter of 0.5-2 cm, or the water drop shape with 1 full terminal bud is cut by classification Or long cylindrical buds, drop-shaped with a diameter of 0.3-1 cm, and long cylindrical with a diameter of 0.3-2 cm and a length of 0.5-1.5 cm, inoculated in the rooting medium, and the remaining part continues to be differentiated. After being cultured in rooting medium for 25-40 days, adventitious roots grow from the transverse stripes of the bud epidermis, the adventitious roots are white, the surface is smooth and there are no fibrous roots, the ...

Embodiment 3

[0041] From the established aseptic bottle seedlings of Taishan Polygonatum high-frequency regeneration system, select aseptic seedlings that grow robustly, have a height of more than 0.5 cm, and have more than one stem node, cut off the complete stem segment from the base, and press at least one plant per node. , the height of each plant is not less than 0.5 cm, and the thickness of the base of the stem segment is more than 0.2 cm. The stem segment is cut, and the base is inserted downward into the rooting medium. After being cultured in the rooting medium for 28-60 days, the growth state of the grafted rhizome segments changed significantly, and the stems and leaves on the upper surface of the medium surface turned yellow until they all died, and at the same time, the base swelled into a drop-shaped aseptic small with one terminal bud. Rhizome single bud, 0.3-0.5 cm in diameter, and grow 1-4 white adventitious roots, the surface of the adventitious root is smooth without fibr...

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Abstract

The invention discloses a Mountain Tai rhizoma polygonati micro rhizome rapid breeding method. Different kinds of differentiation bodies of Mountain Tai rhizoma polygonati serve as test materials to be subjected to induction culture, and can be directly applied to transplanted root-containing micro rhizome. The method can utilize the breeding materials to the maximum, different growing types can all be transformed to micro rhizomes, meanwhile, differentiation and rooting can be achieved synchronously, and the production and breeding efficiency of the Mountain Tai rhizoma polygonati rhizome isremarkably improved. Compared with conventional green seedling transplanting, the method has the remarkable advantages that the procedures are simplified and easy to operate, the cost is lowered, storage and transportation can be conducted conveniently, and the method is a beneficial supplementary of a traditional breeding means, and the method can effectively solve the problems that seedlings ofMountain Tai rhizoma polygonati are scarce, and germplasm sources are not efficiently utilized.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for rapid propagation of Taishan Polygonatum microrhizome. Background technique [0002] Taishan Polygonatum is Asparagaceae ( Asparagaceae ) (originally belonged to Liliaceae ( Liliaceae )) Polygonatum ( Polygonatum ) perennial herb with medicinal value, and Rhizoma Polygonatum ( Polygonatum kingianum ) and Polygonatum ( Polygonatum cyrtonema ) was also included as a medicinal variety by "Chinese Pharmacopoeia 2015". Excessive harvesting and digging have been destroyed, and it is difficult to meet the needs of the market with natural reserves alone, while artificial propagation is mainly based on rhizome propagation, which is prone to germplasm degradation and accumulation of diseases and insect pests, and the reproductive efficiency is low, and seeds are rarely used for propagation. Due to the limited seed yield and long sowing and reproduction cycle, the cu...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A01H4/00A01G22/25
CPCA01H4/008A01G22/25A01H4/001
Inventor黄艳艳郭伟李国华王玉山赵进红王新花张攀仲凤维
OwnerTAISHAN RES INST OF FORESTRY