Preparation of a low-molecular-weight Porphyra laver polysaccharide and its application in anti-human cervical cancer cell tumors

A Porphyra zebra, low molecular weight technology, applied in the field of biochemistry, can solve the problems of different bioavailability, different biological functions, etc.

Active Publication Date: 2021-01-05
温州大学苍南研究院
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, polysaccharides produced by different production processes have different biological functions and bioavailability

Method used

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  • Preparation of a low-molecular-weight Porphyra laver polysaccharide and its application in anti-human cervical cancer cell tumors
  • Preparation of a low-molecular-weight Porphyra laver polysaccharide and its application in anti-human cervical cancer cell tumors
  • Preparation of a low-molecular-weight Porphyra laver polysaccharide and its application in anti-human cervical cancer cell tumors

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Embodiment Construction

[0025] The present invention will be further described in detail below in conjunction with the examples.

[0026] A kind of preparation method of low molecular weight Porphyra zebra polysaccharide

[0027] 1) Dry the frozen Porphyra variegata at 70°C, crush it into powder, and pass through a 40-mesh sieve;

[0028] 2) Reflux the algae powder obtained in step 1 according to the solid-liquid ratio of powder (m):95% ethanol (v)=1:5 at 60°C, degrease for 2 hours, remove ethanol, repeat the above steps twice, and finally dry algae powder;

[0029] 3) Degreasing the defatted algae powder according to the ratio of powder (m): deionized water (v) = 1:30, cooking and extracting at 80°C for 2 hours / time, separating the slag and repeating the above cooking and extracting steps 1 time, collect and combine 2 decoction extracts;

[0030] 4) Concentrating the above decoction extract under reduced pressure at 60°C to 1 / 4 of the total volume of the decoction extract to obtain a concentrated...

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Abstract

The invention relates to preparation of low molecular weight polysaccharides of Porphyra yezoensis and application of the low molecular weight polysaccharides of Porphyra yezoensis in resisting humancervical cancer cell tumors, and belongs to the field of biochemistry. The preparation is technically characterized in that macromolecular polysaccharides of Porphyra yezoensis are extracted by hot water boiling process first and are degraded into small molecular polysaccharides by gamma-ray radiation; the small molecular polysaccharides are mainly applied to resisting human cervical cancer cell tumors. The preparation method provided herein employs simple extraction process and is suitable for industrial production; the polysaccharides of Porphyra yezoensis prepared via the preparation methodhave a good anti-tumor function, are free of cell toxicity and are good in safety and reliability.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a preparation method and application of low-molecular-weight Porphyra zebra polysaccharides. Background technique [0002] Porphyra variegata comes from a wide variety of sources and is found in most coastal regions, including China, Korea, and Japan. It is a nutritious edible seaweed that contains a lot of protein, polysaccharides and other substances. Among them, Porphyra zebra polysaccharides are widely used, involving industrial and medical materials. Porphyra zebra polysaccharides also have biological functions of anti-tumor, anti-virus, and hypolipidemic. In recent years, with the development of biotechnology, the preparation methods of Porphyra zebra polysaccharides are changing with each passing day, including membrane separation method, boiling extraction method (water, lye) and so on. However, most of these preparation methods extract macromolecular polysaccharides. The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
CPCA61K31/715A61P35/00C08B37/0003C08B37/006
Inventor 吴明江何丹佟海滨张旭崔鐘一于萍
Owner 温州大学苍南研究院
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