Kit for qualitative detection of HPV (Human Papilloma Virus) infection in human cervical exfoliated cells

An exfoliated cell, qualitative detection technology, applied in the field of human papillomavirus nucleic acid detection, can solve the problems of inability to clearly distinguish between multiple types, easy to miss other HPV subtypes, etc. Effect

Inactive Publication Date: 2019-03-01
GUANGDONG ASCENDAS GENOMICS TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current method is mainly for HPV6, 11, 16 and 18 infection, it is easy to miss other HPV subtypes, and it cannot clearly distinguish multiple types

Method used

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  • Kit for qualitative detection of HPV (Human Papilloma Virus) infection in human cervical exfoliated cells
  • Kit for qualitative detection of HPV (Human Papilloma Virus) infection in human cervical exfoliated cells
  • Kit for qualitative detection of HPV (Human Papilloma Virus) infection in human cervical exfoliated cells

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Embodiment Construction

[0049] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0050] 1. Determination of nucleic acid types of viruses related to human papillomavirus (HPV)

[0051] During the research process, we found that there are more than 100 types of human papillomavirus (HPV). , 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 70, 72, 73, 81, 82, 89, 83, 67, 55, 84, 34 and other 35 types One or more types of .

[0052] 2. Detection of mutation sites

[0053] 2.1 Detection technology and instruments

[0054] In order to quickly and high-throughput detect these mutations, we use microfluidic technology, including two parts: microfluidic chip and microfluidic platform.

[0055] The microfluidic chip integrates microfluidic channels, valves and reaction chambers on one chip, and automatica...

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Abstract

The invention relates to a kit for detecting human papilloma virus nucleic acid in human cervical exfoliated cells. The kit comprises one or more detection reagents for detecting 35 types such as HP V6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 70, 72, 73, 81, 82, 89, 83, 67, 55, 84, 34 and the like. By using the kit, the types of 35 genes relatedto HPV (Human Papilloma Virus) can be simultaneously detected with high flux under the same reaction condition. The specificity of the detection is strong, the sensitivity is high, the minimum detection limit is 1 ng/uL, and the time is short, wherein the result can be completed within 2 hours from the sample submission to the conclusion.

Description

technical field [0001] The invention relates to the field of human papillomavirus (Human papillomavirus, HPV) nucleic acid detection, more particularly, relates to a kit for qualitative detection of HPV infection in human cervical exfoliated cells. Background technique [0002] Human papillomavirus (human papillomaviruses, HPV) infection is closely related to the occurrence and development of various human tumors, especially cervical cancer. Cervical cancer is one of the most common cancers in women, second only to breast cancer in incidence. There are approximately 400,000 new cases worldwide each year and more than 200,000 women die from the disease. In developing countries, due to the imperfection of cervical cancer screening, its incidence rate is 6 times that of developed countries. In recent years, young cervical cancer patients have an obvious upward trend, and the incidence is increasing at a rate of 2% to 3% per year, causing great harm to women's physical and men...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/708C12Q2561/113C12Q2563/107C12Q2561/101
Inventor 董志强王明璐萧锦华杨呈勇
Owner GUANGDONG ASCENDAS GENOMICS TECH CO LTD
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