A novel cardiac glycoside monomer compound and its use in the preparation of antitumor drugs

A technique for cardiac glycosides and compounds, which is applied in the field of anti-tumor drug screening, can solve the problem that the anti-cancer activity of cardiac glycosides is poorly understood, and achieve the effects of strengthening in vitro anti-cancer activity, inhibiting cell proliferation, and inducing cell apoptosis.

Active Publication Date: 2022-04-05
TIANJIN UNIV OF SCI & TECH
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Problems solved by technology

However, little is known about the possible anticancer activity of cardiac glycosides and some of their derivatives

Method used

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  • A novel cardiac glycoside monomer compound and its use in the preparation of antitumor drugs
  • A novel cardiac glycoside monomer compound and its use in the preparation of antitumor drugs
  • A novel cardiac glycoside monomer compound and its use in the preparation of antitumor drugs

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Embodiment Construction

[0026] The present invention will be further described in detail below in conjunction with the accompanying drawings and through specific embodiments. The following embodiments are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0027] One, the present invention has confirmed through experiments that the novel cardiac glycoside monomer compound extracted from the traditional Chinese medicine Xiaotaoerqi has anticancer activity, and it is named HTF-1, the method is as follows:

[0028] First, the MTT assay was used to confirm that HTF-1 can induce apoptosis in human cervical cancer HeLa cells, human breast cancer MCF-7 cells, and human liver cancer HepG2 cells in a dose- and time-dependent manner.

[0029] Then HeLa cells were taken as the research object, and the apoptosis, cell cycle, proliferation, Migration, invasion, and the expression levels of related proteins.

[0030] The research results show that HTF-1 can inhibit...

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Abstract

The invention relates to a novel cardiac glycoside monomer compound and its application in the preparation of antitumor drugs. It is confirmed by MTT experiment that HTF-1 can induce human cervical cancer HeLa cells, human breast cancer MCF-7 cells, and human liver cancer HepG2 Cell apoptosis occurs in a dose- and time-dependent manner. Then HeLa cells are used as the research object, and the effects of HTF‑1 on HeLa cells are detected by using cell flow analysis technology, wound healing experiment, Transwell chamber experiment, and western blotting. Apoptosis, cell cycle, proliferation, migration, invasion and the expression levels of related proteins. In the present invention, HTF‑1 can inhibit the proliferation, migration and invasion of cancer cells, induce HeLa cells to undergo apoptosis, and make the cell cycle stagnate in the S phase.

Description

technical field [0001] The invention belongs to the field of antitumor drug screening, in particular to a novel cardiac glycoside monomer compound and its application in the preparation of antitumor drugs. Background technique [0002] Tumor is one of the most serious diseases that endanger human health. Traditional chemotherapy and radiotherapy have successfully improved the cure rate of various malignant tumors and alleviated the pain of patients. But at the same time, it also has disadvantages such as strong side effects and easy recurrence after operation. Traditional Chinese medicine is the material basis of traditional Chinese medicine treatment. It has the characteristics of multi-component and multi-target effects, and can act on different links of tumor pathogenesis. At the same time, it has low toxic and side effects, can improve the body's immunity, and is not easy to produce drug resistance, etc. These advantages make it urgent to search for new antitumor drugs ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J19/00A61K31/585A61P35/00
CPCA61P35/00C07J19/005
Inventor 马龙苏艳芳满淑丽
Owner TIANJIN UNIV OF SCI & TECH
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