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A chiral analysis method for nornicotine, pseudobasine and anatabine in tobacco and tobacco products

A technology for tobacco products and pseudobaitine, which is applied in the fields of analysis of materials, material separation, instruments, etc., and can solve problems such as difficult to achieve routine laboratory analysis, incomplete R/S chiral separation, etc.

Active Publication Date: 2021-08-20
GUIZHOU TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method also uses the derivatization method of trifluoroacetylation, and the R / S chiral separation of the three substances is not complete
In addition, multidimensional gas chromatography requires special instruments, and it is difficult to achieve routine analysis in the laboratory

Method used

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  • A chiral analysis method for nornicotine, pseudobasine and anatabine in tobacco and tobacco products
  • A chiral analysis method for nornicotine, pseudobasine and anatabine in tobacco and tobacco products
  • A chiral analysis method for nornicotine, pseudobasine and anatabine in tobacco and tobacco products

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Experimental program
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Effect test

Embodiment 1

[0020] Example 1: Chiral Analysis of Nornicotine, Pseudine and Anatabine

[0021] According to the chiral analysis method of nornicotine, anatabine and anatabine in a kind of tobacco and tobacco products of the present invention, refer to figure 1 , including the following steps:

[0022] A. Extraction of nornicotine, anatabine and anatabine in tobacco and tobacco products: after crushing the tobacco sample through a 80-100 mesh sieve, weigh 200mg of tobacco sample powder into a 50ml centrifuge tube, add 50μL of dichloro Prepared in methane to a concentration of 5.4mg mL -1 4-phenylpiperidine internal standard solution, and 1mL 5% sodium hydroxide solution, vortexed and mixed, then added 3mL of dichloromethane 2500rpm vortex extraction for 40min, centrifuged at 4000rpm for 5min, and took the lower layer for later use;

[0023] B. Derivatization of nornicotine, anatabine, and anatabine in tobacco and tobacco products: Take 400 μL of the middle and lower layer of A as the extr...

Embodiment 2

[0026] Embodiment 2: linearity, accuracy, precision and sensitivity verification of the method of the present invention

[0027] Prepare the mother liquor of corresponding concentration with dichloromethane (racemate RS-nornicotine 0.52mg mL -1 , RS-Anatabine 0.72mg mL -1, RS-Pseudine 0.95mg mL -1 ), take 2, 5, 10, 25, 50, and 100 μL of the mother solution respectively, and add 10 μL of 5.4 mg mL to the 6 gradient mixed standard solutions -1 4-phenylpiperidine internal standard, and finally the mixed solution was distilled to 400 μL with dichloromethane, derivatized and extracted according to the method in B, and then analyzed by gas chromatography-nitrogen and phosphorus detector. The standard curve was based on the target compound The ratio of the peak area to the peak area of ​​the internal standard is the ordinate (Y, since the amount of the internal standard added in the sample is 5 times that of the internal standard in the standard curve, the ratio of the peak area of...

Embodiment 3

[0031] Example 3: Chiral Analysis of Nornicotine, Pseudine and Anatabine in Tobacco Leaves with Different Nicotine Contents

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Abstract

The invention discloses a chiral analysis method for nornicotine, anatabine and anatabine in tobacco and tobacco products, which uses an alkaline organic solvent to extract nornicotine, anatabine and anatabine in tobacco , and then use the dichloromethane solution of (1S)‑(‑)‑camphoryl chloride for simultaneous derivation and extraction, and the extracted nornicotine, pseudobasine and anatabine are directly processed by gas chromatography‑nitrogen phosphorus detector. qualitative and quantitative separation of chirality; the present invention has the advantages of simple and fast, high accuracy and sensitivity, and good separation ability of chiral S-(-)- and R-(-)-alkaloids (R S >1.5), it can simultaneously analyze the chirality of nornicotine, anatabine and anatabine in tobacco and tobacco products.

Description

technical field [0001] The invention relates to a chiral analysis method for nornicotine, anatabine and anatabine in tobacco and tobacco products, and belongs to the technical field of determination methods for chemical components of tobacco. Background technique [0002] Nornicotine, anatabine, and anatabine in tobacco have been widely considered to be closely related to the quality of tobacco, and are also precursors of highly carcinogenic substance-specific nitrosamines. In flue-cured tobacco samples, nornicotine and anatabine accounted for about 2-3%, and anatabine accounted for about 0.3%, which are the most important alkaloids except nicotine. In addition, nornicotine, anatabine, and anatabine are tobacco-specific subspecies, such as nitrosonornicotine (NNN), nitrosoanabatin (NAT), and nitrosoanatabine (NAB), respectively. The immediate precursor of nitrosamines, tobacco-specific nitrosamines, is the most abundant, most carcinogenic and most concerned harmful componen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 蔡凯潘文杰高维常赵会纳雷波张继
Owner GUIZHOU TOBACCO SCI RES INST
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