Application of circ-PLEKHA1 molecular marker in blood to diagnosis of ESCC (esophageal squamous carcinoma)

A technology of circ-fam193a and molecular markers, applied in the field of tumor molecular biology, can solve the problems of large trauma and unacceptable patients

Inactive Publication Date: 2019-04-09
江苏万成生物医学研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in esophageal squamous cell carcinoma, circRNAs are rarely reported
At present, ESCC does not have plasma tumor markers to evaluate the condition. Endoscopy is the gold standard, but it is traumatic and difficult for patients to accept.

Method used

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  • Application of circ-PLEKHA1 molecular marker in blood to diagnosis of ESCC (esophageal squamous carcinoma)
  • Application of circ-PLEKHA1 molecular marker in blood to diagnosis of ESCC (esophageal squamous carcinoma)
  • Application of circ-PLEKHA1 molecular marker in blood to diagnosis of ESCC (esophageal squamous carcinoma)

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Embodiment 1

[0031] 1. RNase R Digestion and Actinomycin-D Digestion

[0032] 2mg of total RNA was incubated at 37°C for 20 minutes with or without the addition of 3U / mg RNase R, followed by the use of the RNeasyMinElute kit. For actinomycin D treatment, 2 mg of total RNA was incubated at 37°C with or without adding 1 mg of actinomycin (actinomycin D, purchased from Sigma Reagent Company), and at 0, 6, 12, The resulting RNA was detected separately for 18 hours.

[0033] 2. Subjects

[0034] This study is based on the Department of Oncology, Nanjing Hospital Affiliated to Nanjing Medical University. The Department of Oncology treats patients with esophageal squamous cell carcinoma all the year round, and establishes a specimen bank, which can provide rich case data for this study. The tissues and blood of the study patients came from patients with esophageal squamous cell carcinoma who underwent radical or palliative surgical resection from 2013 to August 2017. The normal control blood s...

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Abstract

The invention belongs to the field of oncomolecularbiology and particularly relates to an application of a circ-PLEKHA1 molecular marker in blood to diagnosis of ESCC (esophageal squamous carcinoma).A detection reagent for the circ-PLEKHA1 molecular marker can be used for preparing a diagnostic reagent for ESCC; circular RNA has the characteristics of stable mechanism, high abundance and sample specific expression, expression quantities of circ-PLEKHA1 in blood samples of an ESCC patient and a normal person are different and related to stages, and a method is easily accepted by subjects and can be effective means for early diagnosis and disease progression judgment of the ESCC patient.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and in particular relates to the application of circ-PLEKHA1 molecular marker in blood in the diagnosis of esophageal squamous cell carcinoma. Background technique [0002] Esophageal cancer is one of the most common malignant tumors that seriously threaten human health. There are two main pathological types: squamous cell carcinoma and adenocarcinoma. Ninety percent of esophageal cancers are squamous cell carcinomas (ESCC), and their distribution has obvious geographical differences. The incidence of ESCC in my country accounts for more than 70% of the world, and the prognosis is poor. The 5-year overall survival rate is only 15%-25%. In 2016, the Journal of Clinician Cancer reported that esophageal cancer has become one of the five most common tumors in China, ranking third among men and fifth among women. [0003] Circular RNAs (circRNAs), a new type of endogenous non-coding RNA, were ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 康宁朱桂芳曹秀峰
Owner 江苏万成生物医学研究院有限公司
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