Biomarker for early breast cancer diagnosis

A breast cancer, drug technology, applied in the field of biomedicine

Inactive Publication Date: 2019-04-16
赣南医学院第一附属医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of lncRNAs is very large, only a few cancer-related lncRNAs have

Method used

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  • Biomarker for early breast cancer diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Screening for Gene Markers Related to Early Breast Cancer

[0042] 1. Sample collection

[0043] 30 breast cancer tissue samples and corresponding paracancerous tissue samples were collected from 30 cases of stage I-II, and 5 cases were randomly selected for high-throughput sequencing. All cases did not receive chemotherapy and radiotherapy before surgery to exclude other tumors. Diseases, autoimmune diseases, and severe chronic diseases, all patients gave informed consent and were approved by the organization's ethics committee.

[0044] 2. Preparation of RNA samples

[0045] Cut the tissue into pieces with scissors, add 1ml Trizol, shake on the shaker for 1min; place at room temperature for 10min to completely decompose the ribosomes; then add 200μl chloroform (chloroform), close the tube cap tightly, shake vigorously for 15s, and let stand at room temperature for 10min Finally, centrifuge at 11000rpm for 15min at 4°C; transfer the water sample layer to a ...

Embodiment 2

[0056] Example 2 QPCR sequencing verification of differential expression of LINC01929 gene

[0057] 1. Using the previously collected tissue samples of 30 cases of stage I-II breast cancer and paracancerous tissue samples to conduct large-scale QPCR verification of LINC01929.

[0058] 2. The RNA extraction operation steps are the same as in Example 1

[0059] 3. QPCR

[0060] 1) Reverse transcription reaction

[0061] The lncRNA reverse transcription was carried out using the FastQμant cDNA First Strand Synthesis Kit (Product No.: KR106) from Tiangen Company.

[0062] First remove the genomic DNA reaction, add 5×gDNA Bμffer 2.0μl in the test tube, total RNA 1μg, add RNase Free ddH 2 O Make the total volume to 10μl, heat in a water bath at 42°C for 3min, then add 10×Fast RT Bμffer 2.0μl, RT Enzyme Mix 1.0μl, FQ-RT Primer Mix 2.0μl, RNase Free ddH 2 O 5.0 μl, after mixing, add to the above test tube and mix together a total of 20 μl, heat in a water bath at 42°C for 15min, t...

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Abstract

The invention discloses a biomarker for early breast cancer diagnosis. The biomarker is LINC01929. By the detection of tissue samples and paracancerous tissues of breast cancer in stage I-II, it is found that the expression level of LINC01929 in cancer tissues is significantly up-regulated, suggesting that LINC01929 can be used for the diagnosis and treatment of early breast cancer.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a biomarker for early breast cancer diagnosis, specifically the biomarker is LINC01929. Background technique [0002] Breast cancer is one of the most common malignant tumors in women, usually occurring in the breast epithelial tissue. According to statistics, the incidence of breast cancer accounts for 7-10% of all kinds of malignant tumors in the whole body (Darabi, H et al. Breastcancer risk prediction and individualized screening based on common genetic variation and breast density measurement. Breast Cancer Res, 2012.14 (1) :p.R25.). There are 1.35 million new breast cancers in the world every year, of which 420,000 die, and the annual increase is 2% (Wen, W., et al., Prediction of breast cancer risk based on common genetic variants in women of East Asian ancestry. Breast Cancer Res , 2016.18(1): p.124.). Every year, more than 40,000 women die of this disease in China. Although it...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11A61K45/00A61P35/00
CPCA61K45/00A61P35/00C12Q1/6886C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 罗忠兵
Owner 赣南医学院第一附属医院
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