Preparation method and application of immobilized enzyme of providencia stuartii

A technology of Providencia stutzeri and Providencia, applied in the field of biotechnology degradation of pesticide residues

Pending Publication Date: 2019-05-14
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, no research work has been carried out on the biodegradation of sclerotin

Method used

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  • Preparation method and application of immobilized enzyme of providencia stuartii
  • Preparation method and application of immobilized enzyme of providencia stuartii

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Screening of immobilized carriers:

[0019] Preliminary experiments showed that in 1%, 2%, 3%, 4%, 5% sodium alginate (SA) fixed suspension free enzyme, 3% SA showed better effect; in 1.5%, 2%, 2.5% %, 3%, 3.5% polyethylene glycol (PVA) fixed suspension free enzyme, 2.5% SA showed a better effect. Further, the experiments determined the degradation effects of free enzymes, 3% SA+ free enzymes, 2.5% PVA+ free enzymes, 3% SA+2.5% PVA+ free enzymes on 50 mg / L of sclerotinia, and the results are shown in figure 1 .

[0020] 2. Immobilized enzyme preparation:

[0021] 1) Bacterial suspension preparation: Add 2 mL of sterile phosphate buffer under aseptic conditions and shake in parallel in the culture dish cultured for 48 hours, and gently scrape off the colonies on the surface of the medium with an inoculation loop to form a crude bacterial suspension. liquid, and transferred it to a liquid fermentation medium (beef extract 8g / L, yeast extract 14g / L, NaCl 5g / L) and cu...

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Abstract

The invention discloses a preparation method and application of an immobilized enzyme of providencia stuartii. According to the immobilized enzyme of providencia stuartii, with providencia stuartii CCTCC M 2017738 as a strain and sodium alga acid as an immobilization material, a providencia stuartii enzyme solution through bacterium suspension propagation expanding and degrading enzyme ultrasonication is effectively wrapped so as to prepare the immobilized enzyme. The immobilized enzyme can be applied to efficient degradation of dimethachlon, vinclozolin and iprodione in soil and water. The immobilized enzyme has the advantages of being good in persistence and efficacy performance, environmentally friendly, obvious in effect, low in cost, easy to popularize and the like.

Description

technical field [0001] The invention relates to the field of biotechnological degradation of pesticide residues, in particular to a preparation method and application of an immobilized enzyme of Providencia stutzeri. Background technique [0002] Pesticides play an important role in the integrated management of agricultural pests, but pesticide residues and their metabolites have become serious pollutants, posing threats to human health and ecosystems. Dimetachlone, also known as Dimetachlone, has molecular formula C 10 h 7 Cl 2 NO 2 , its relative molecular mass is 244.07, and its chemical name is: N-(3,5-dichlorophenyl)succinimide. In my country, it is widely used in the prevention and control of fungal diseases such as sclerotinia, sheath blight, and red spot on rapeseed, Chinese cabbage, laver moss, lettuce, leek, cucumber, tomato, pepper, potato, tobacco and other crops. Despite its promising bactericidal activity, sclerotinia is currently banned in the US and Euro...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12N11/04B09C1/10C02F3/34C12R1/01C02F101/38
Inventor吴小毛张承李荣玉龙友华李姣红王秋萍黄文源代园凤
OwnerGUIZHOU UNIV