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Nano-sheets containing nitrogen monoxide donor as well as preparation method and application of nano-sheets

A technology of nitric oxide and nanosheets, applied in the field of medicine, can solve problems such as the inability to guarantee tumor delivery of nitric oxide, and achieve the effect of a simple method

Active Publication Date: 2019-05-28
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of this, the purpose of the present invention is to solve the problem that the effective delivery of nitric oxide to tumors cannot be ensured in the prior art, and to provide a nanomaterial that can deliver nitric oxide to tumors

Method used

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  • Nano-sheets containing nitrogen monoxide donor as well as preparation method and application of nano-sheets
  • Nano-sheets containing nitrogen monoxide donor as well as preparation method and application of nano-sheets
  • Nano-sheets containing nitrogen monoxide donor as well as preparation method and application of nano-sheets

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Experimental program
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Effect test

Embodiment 1

[0057] Embodiment one: Synthesis and characterization nanosheet ZnNO:

[0058] Such as figure 1 As shown, synthesize nanosheets by inverse microemulsion method: the first step, surfactant Igepal CO-520 is added in cyclohexane (volume ratio of Igepal CO-520 and cyclohexane=2:5), Mix evenly to form a microemulsion. Add 0.4 moles per liter of sodium nitroprusside solution into the microemulsion (v / v=1 / 50) to form the anion phase. Adding 0.1-0.4 moles per liter of zinc ion solution into the microemulsion (v / v=1 / 50) constitutes the cationic phase. Control the temperature at 25-35° C., add an equal volume of the anion phase to the cation phase dropwise, and mix and stir overnight. In the second step, the nanomaterials are precipitated by centrifugation, and then the nanomaterials are washed several times with organic solvents such as ethanol.

[0059] The nanosheets were then characterized, electron microscope images such as figure 2 It is shown that the nanomaterial is a two...

Embodiment 2

[0061] Example 2: Nanosheets Label Nuclides and Induce Nitric Oxide Release

[0062] Ultrasonic dispersion of nanosheets in aqueous solution, addition of radionuclides 32 P, shaking in a shaker at 50°C for 1 hour, and centrifuging to remove free nuclides in the supernatant to obtain ZnNO ( 32 P). The stability of the radiolabel was tested with results such as Figure 8 As shown, ZnNO( 32 P) has high radiolabeling stability. Disperse again in an aqueous solution, place it under dark conditions, take the solution and centrifuge at different time points, and test the concentration of nitric oxide in the supernatant. Such as Figure 9 Nuclides were shown to induce the release of nitric oxide.

Embodiment 3

[0063] Embodiment three: the killing of cancer cell by nuclide-labeled nanosheets:

[0064] ZnNO with different concentrations and radioactive doses ( 32 P) Incubate with mouse colon cancer cells, and measure cell viability after 72 hours. The result is as Figure 10 As shown, blank nanosheets had no significant cytotoxicity, while ZnNO( 32 P) has a strong cell killing effect. The concentration of nitric oxide in the supernatant of the cell culture medium of the last group was detected as Figure 11 , ZnNO( 32 Group P) has a high concentration of nitric oxide.

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Abstract

The invention belongs to the field of medicines, and discloses nano-sheets containing a nitrogen monoxide donor as well as a preparation method and application of the nano-sheets. The nano-sheets disclosed by the invention are prepared from a nitrogen monoxide donor medicine and metal ions. The method for synthesizing the nano-sheets is simple and a lot of the nano-sheets can be prepared. The nano-sheets synthesized by the method can be used for directly marking nuclide without a chelating agent. According to the nano-sheets synthesized by the method, nitrogen monoxide is can be realized through Cherenkov fluorescent light of the nuclide and external stimulation is not needed. After the nano-sheets disclosed by the invention are marked by radioactive nuclide, the nuclide induced Cherenkovfluorescent light can be used for releasing the nitrogen monoxide and a tumor microenvironment is adjusted; the tumor radiotherapy and immunotherapy effects are enhanced and the nano-sheets can be used for preparing a treatment agent for treating cancers.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a nanosheet and its preparation method and application, especially the application in preparing a therapeutic agent for cancer. Background technique [0002] Cancer causes millions of deaths worldwide every year and is one of the most dangerous diseases facing mankind today. The combination of traditional radiotherapy and emerging immunotherapy has great clinical prospects and applications in the field of tumor treatment. Studies have found that after radiation therapy, tumors in unirradiated areas also regress, which is called the distal effect. Further studies have shown that radiotherapy leads to immunogenic apoptosis of tumor cells and activates T cell tumor immunity. Immune checkpoint inhibitor therapy also treats cancer by activating T cell immunotherapy, so the combination of radiation therapy and immune checkpoint inhibitors has strong scientific significance. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K51/02A61P35/00A61K101/00
Inventor 刘庄田龙龙
Owner SUZHOU UNIV
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