A parasite staining solution that does not require fixation

A parasite and dyeing solution technology, applied in the field of parasite dyeing solution, can solve the problems such as physical injury of staff, incomplete shape, lower detection rate, etc., and achieve the effects of simple and fast method, clear coloring, and high detection rate

Active Publication Date: 2021-03-02
HUBEI TAIKANG MEDICAL EQUIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The birth of histopathology has a history of more than 150 years. Eosin solution is one of the commonly used pathological reagents. It is mainly used to stain tissue sections. Most of the current eosin staining solutions contain formaldehyde, xylene, mercuric oxide, Ammonia, etc., are toxic chemical reagents, not only causing harm to the health of the staff, but also polluting the environment
[0003] Single coloring for parasites, unclear coloring, incomplete shape, and easy to identify a single type of parasite, while other types of parasites are not easy to identify, reducing the detection rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] 1) Add 5% by weight of sodium chloride into water at a temperature of 30°C, stir evenly, heat up to 51°C, add 5% by weight of eosin, 2% by weight of azure, 3 % by weight of methylene blue, 0.5% by weight of tocopherol, 0.2% by weight of nano-pearl powder, and 0.6% by weight of ethyl acetate, stirred evenly, and then ultrasonicated for 5min at a water bath temperature of 32°C to obtain the first solution;

[0082] 2) Add 0.01% by weight of neutral glycerin to 10% by weight of methanol, sonicate for 2 minutes at a water bath temperature of 35°C, then add 1% by weight of Tween-20, and sonicate for 1 minute at a water bath temperature of 40°C , to obtain the second solution;

[0083] 3) The first solution in step 1) and the second solution in step 2) were mixed into a vessel, and the vessel was sonicated in a water bath at a water bath temperature of 33° C. for 1 min to obtain a parasite staining solution.

[0084] Water is distilled water.

Embodiment 2

[0086] 1) Add 6% by weight of sodium chloride into water at a temperature of 39°C, stir evenly, heat up to 55°C, add 8% by weight of eosin, 5% by weight of azure, 6 % by weight of methylene blue, 1% by weight of tocopherol, 0.8% by weight of nano pearl powder, and 0.9% by weight of ethyl acetate, stirred evenly, and then ultrasonicated for 8 minutes at a water bath temperature of 35°C to obtain the first solution;

[0087] 2) Add 0.02% by weight of neutral glycerin to 50% by weight of methanol, sonicate for 3 minutes at a water bath temperature of 35°C, then add 2% by weight of Tween-20, and sonicate for 2 minutes at a water bath temperature of 40°C , to obtain the second solution;

[0088] 3) The first solution in step 1) and the second solution in step 2) were mixed into a vessel, and the vessel was sonicated in a water bath at a water bath temperature of 33° C. for 3 minutes to obtain a parasite staining solution.

[0089] Water is deionized water.

Embodiment 3

[0091] 1) Add 5.6% by weight of sodium chloride into water at a temperature of 35°C, stir evenly, heat up to 54°C, add 5% by weight of eosin, 3% by weight of azure, 4 % by weight of methylene blue, 0.6% by weight of tocopherol, 0.5% by weight of nano-pearl powder, and 0.8% by weight of ethyl acetate, stirred evenly, and then ultrasonicated for 7 minutes at a water bath temperature of 33°C to obtain the first solution;

[0092] 2) Add 0.01% by weight of neutral glycerin to 200% by weight of methanol, sonicate for 3 minutes at a water bath temperature of 35°C, then add 1% by weight of Tween-20, and sonicate for 2 minutes at a water bath temperature of 40°C , to obtain the second solution;

[0093] 3) The first solution in step 1) and the second solution in step 2) were mixed into a vessel, and the vessel was sonicated in a water bath at a water bath temperature of 33° C. for 1-3 minutes to obtain a parasite staining solution.

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PUM

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Abstract

The invention discloses a parasite staining solution without fixation, which comprises the following components by weight: 5-8% eosin; 2-5% azure; 3-6% methylene blue; 10-50% methanol; Glycerin 0.01~0.02%; Sodium Chloride 5~6%; Tween-201~2%; Tocopherol 0.5~1%; Nano Pearl Powder 0.2~0.8%; Ethyl Acetate 0.6~0.9%; The present invention is applicable to the dyeing of parasites, various microorganisms, blood cell smears, bone marrow smears, exfoliated cell smears, chromosome banding, cell apoptosis detection, etc., and only uses one dye solution without fixing, fixing and staining Combining two into one, multiple inspections such as parasites, germs, nuclear heterogeneous cells, and cell classification and counting can be performed on one film at the same time. The method is simple and fast, with strong staining power, clear coloring, complete shape, and easy to identify and detect High rate.

Description

technical field [0001] The invention relates to the technical field of hygienic preparations, in particular to a parasite staining solution without fixation. Background technique [0002] The birth of histopathology has a history of more than 150 years. Eosin solution is one of the commonly used pathological reagents. It is mainly used to stain tissue sections. Most of the current eosin staining solutions contain formaldehyde, xylene, mercuric oxide, Ammonia, etc., are toxic chemical reagents, which not only cause harm to the health of the staff, but also pollute the environment. [0003] The coloring of parasites is single, the coloring is not clear, the shape is incomplete, and it is easy to identify a single type of parasite, while other types of parasites are not easy to identify, which reduces the detection rate. Contents of the invention [0004] The invention provides a parasite staining solution without fixation. [0005] The scheme of the present invention is: ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
Inventor 肖国林
Owner HUBEI TAIKANG MEDICAL EQUIP
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