A method for Arabidopsis thaliana pepr2 protein and atpep1 small peptide to synergistically inhibit geminivirus infection

Abstract

The invention discloses a method for inhibiting Geminivirus infection through the synergistic effect of Arabidopsis PEPR2 protein and AtPep1 small peptide, and specifically relates to the field of methods for inhibiting Geminivirus infection. Plants; step 2, overexpressing PEPR2 in the plants, and at the same time, exogenously applying AtPep1 small peptides to synergistically activate the anti-virus mechanism. The present invention utilizes the synergistic method of plant-derived genes and small peptides to improve the resistance of plants to geminiviruses, and solves the problem that geminiviruses cause serious harm to crop production in my country. The regulation of the encoded C4 protein has developed a specific and controllable plant disease resistance method. Since C4 is a pathogenic factor with a conserved sequence in a variety of geminiviruses, this technology can be applied to other geminivirus species to improve future Virus resistance of crops.

Description

technical field [0001] The invention relates to the technical field of methods for inhibiting geminivirus infection, and more specifically, the invention relates to a method for inhibiting geminivirus infection through the synergistic effect of Arabidopsis thaliana PEPR2 protein and AtPep1 small peptide. Background technique [0002] Geminiviruses are a class of plant DNA viruses with a wide range of hosts, and many important crops are threatened by them. Geminiviruses have been found in food and economic crops in many provinces of my country, causing harm to agricultural production. Therefore, an in-depth study of the interaction between geminiviruses and plants is of great significance for preventing and controlling diseases, improving crop yields, and ensuring the development of agricultural production. [0003] Geminiviruses have a single-stranded DNA genome that encodes only a few proteins, and complete their life cycle by infecting and interacting with host cells. Af...

AI Technical Summary

Problems solved by technology

[0010] The main problem of this technology is: the overexpression of LSB1 / GDU3 gene itself constitutively and continuously activates the disease resistance pathway of plants, and the continuous accumulation of disease resistance signals leads to the influence of plant growth and development under normal conditions; at the same time, the resistance of this method The mechanism of action of the virus is unknown, and it may cause other unknown side effects during application

[0011] In addition, there are few effective prevention and treatment technologies for geminiviruses, which mainly use overexpression or knockdown of plant genes to activate related signaling pathways to improve resistance to viruses, and gene editing for viral DNA to disrupt the infection process; among them Overexpression and knockdown of plant genes mainly activate broad disease resistance pathways such as salicylic acid constitutively, or control virus replication by affecting plant cell cycle. Therefore, while improving disease resistance, the growth and development of the plant itself It may also have an impact; although the gene editing technology for viral DNA specifically destroys the structural integrity of the virus itself, existing studies have shown that the current technology may have off-target phenomena, which may have an impact on the genome of the plant itself

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