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MSTN nano antibody, construction method and application thereof

A nanobody and construction method technology, applied in the field of biomedicine, can solve the problems of non-MSTN antibody application, large library capacity and high cost

Active Publication Date: 2019-06-28
SHIHEZI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] (3) No MSTN antibody has been applied to production in the prior art
[0011] (4) The production cost of MSTN antibody by existing technology is relatively high, and there are problems such as antibody heterogeneity
But the library capacity is larger than other phages

Method used

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  • MSTN nano antibody, construction method and application thereof
  • MSTN nano antibody, construction method and application thereof
  • MSTN nano antibody, construction method and application thereof

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Embodiment Construction

[0085] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0086] In the prior art, there is no theoretical support for improving the muscle growth-promoting effect of sheep MSTN nanobody.

[0087] Below in conjunction with accompanying drawing, the application principle of the present invention is described in detail;

[0088] Such as figure 1 As shown, the MSTN nanobody provided by the embodiments of the present invention and its application method are:

[0089] S101: Constructing a nanobody library of sheep MSTN gene expression products;

[0090] S102: Obtain specific nanobodies against MSTN gene expression products through phage display technology;

[0091] S103: Constructi...

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Abstract

The invention belongs to the technical field of biological medicines, and discloses an MSTN nano antibody, a construction method and application thereof. Constructing a nano antibody library of sheepMSTN gene expression product; obtaining a specific nano antibody aiming at an MSTN gene expression product through a phage display technology; and constructing a bivalent sheep MSTN nano antibody; theMSTN nano antibody has the functions of increasing animal muscle content and reducing animal fat content on mice. When the weight gain was mainly muscle weight gain, the sheep MSTN nano antibody showed that the weight gain was higher than that of untreated control group; mice showed that the muscle weight of the nano antibody group was significantly higher than that of the control group, and thepercentage of muscle in the whole body was also significantly higher than that of the control group. Sheep MSTN nano antibodies can promote muscle growth in animals.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an MSTN nanobody, a construction method and an application thereof. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] MSTN is a gene that regulates muscle growth in animals. Mainly play a role in inhibiting muscle growth. Loss of MSTN can promote double-muscle traits in animals. When the function of sheep MSTN gene is disturbed, it can promote the growth of sheep muscle, and can better provide more mutton for people of all nationalities. Through the principle of antigen-antibody reaction, the preparation of antibodies to inhibit the action pathway of MSTN can inhibit the inhibition of muscle growth by MSTN gene expression products, shorten the fattening time of sheep and increase the muscle output. Ma Xianyong et al. amplified the pig (MSTN) gene, and the crude extract of the expressed re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/22C12N15/70C12P21/06G01N33/68
Inventor 陈创夫盛金良吴鹏李尤简郭吉星
Owner SHIHEZI UNIVERSITY
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