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Method for researching polymorphism of different geographical populations cpDNatnT-trnL of Snowfox Crested wheatgrass

A technology of wheatgrass and polymorphism, applied in the field of biological genetics

Inactive Publication Date: 2019-07-26
青海省铁卜加草原改良试验站
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  • Application Information

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Problems solved by technology

There is a large area of ​​Agrocystis platyscens distributed in the region around Qinghai Lake on the Qinghai-Tibet Plateau. There are many studies on A. platyscens in terms of gene cloning and expression, as well as strain selection and genetic diversity. For its cpDNAtrnT- The study of trnL sequence is rarely reported

Method used

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  • Method for researching polymorphism of different geographical populations cpDNatnT-trnL of Snowfox Crested wheatgrass
  • Method for researching polymorphism of different geographical populations cpDNatnT-trnL of Snowfox Crested wheatgrass
  • Method for researching polymorphism of different geographical populations cpDNatnT-trnL of Snowfox Crested wheatgrass

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Experimental program
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Embodiment 1

[0030] In this experiment, 6 populations were selected as the main production areas of Agrocystis spp. collected in 2013 from the lake-surrounding areas of the Qinghai-Tibet Plateau in Hercynian, Haibei and Hainan regions. When sampling, 9-20 representative adult plants were selected for each population. According to the principle of random sampling, the distance between individuals was 5-10m. Collect fresh and complete young leaves of plants in the wild, and store them dry in plastic bags filled with silica gel. The geographic location, latitude and longitude, and sample size are detailed in Table 1.

[0031] Table 1. The longitude, latitude, altitude, haplotype diversity and nucleotide diversity of the six Agrocystis flatus populations

[0032]

[0033]

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Abstract

The invention discloses a method for researching polymorphism of different geographical populations cpDNatnT-trnL of Snowfox Crested wheatgrass. The method includes the steps: collecting a plurality of tender leaves of the Snowfox Crested wheatgrass in a wheatgrass population; extracting the total DNA of each tender leaf of the Snowfox Crested wheatgrass; amplifying the trnT-trnL fragment of the cpDNA of each tender leaf of the Snowfox Crested wheatgrass to obtain a plurality of amplification products; carrying out sequencing to obtain a nucleotide sequence of a trnT-trnL amplified fragment ofthe cpDNA of each tender leaf of the Snowfox Crested wheatgrass; analyzing the sequence data by adopting software; and according to data analysis, obtaining cpDNAtrnT-trnL polymorphism information ofthe Snowfox Crested wheatgrass in different geographical populations, including sequence variation, genetic diversity, genetic structure of populations and phylogenetic relationship information amongthe populations. The method for researching polymorphism of different geographical populations cpDNatnT-trnL of Snowfox Crested wheatgrass can accurately obtain the polymorphism information of different geographical populations of the Snowfox Crested wheatgrass, and can provide a scientific basis for species protection.

Description

technical field [0001] The invention relates to the technical field of biological genetics, in particular to a research method for cpDNAtrnT-trnL polymorphisms of different geographic populations of Agropyresis platyphoid. Background technique [0002] Genetic diversity is the molecular, cellular, and individual genetic variation within or between species, and is the basic condition for species to maintain evolutionary potential and resist adverse environments. The genetic diversity of organisms, which provides the raw material for evolution and potential adaptation to changing environments, has received great attention in conservation biology. As an important part of biodiversity, genetic diversity reflects the diversity of species and above to a certain extent. Generally speaking, genetic diversity refers to the genetic variation between different groups within a species or among different individuals within a group. High genetic diversity is the basis for maintaining th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/20G16B25/20G16B25/10G16B30/10G16B40/00G16B40/10C12Q1/6806
CPCG16B20/20G16B25/20G16B25/10G16B30/10G16B40/00G16B40/10C12Q1/6806C12Q2531/113
Inventor 周学丽土旦加史德军
Owner 青海省铁卜加草原改良试验站
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