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Glyphosate-resistant Paenibacillus colioids jd-07, bacterial agent and application

A JD-07, Paenibacillus technology, applied in applications, bacteria, microorganism-based methods, etc., can solve the problem of limited tolerance to the herbicide glyphosate, affecting the application and function of functional strains

Active Publication Date: 2020-12-18
北京航天恒丰科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Paenibacillus colioids has limited tolerance to the herbicide glyphosate. Studies have shown that the tolerance of Paenibacillus colioids to glyphosate is only 3mmol / L, and Paenibacillus colioids will be higher than 3mmol / L. Stop growing, seriously affecting the application and function of the functional strain

Method used

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  • Glyphosate-resistant Paenibacillus colioids jd-07, bacterial agent and application
  • Glyphosate-resistant Paenibacillus colioids jd-07, bacterial agent and application
  • Glyphosate-resistant Paenibacillus colioids jd-07, bacterial agent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Determination of the concentration of Paenibacillus colioids resistant to glyphosate isopropylamine salt

[0054] The activated Paenibacillus colioids were inoculated into ACCC55 containing different concentrations of glyphosate isopropylamine salt (1mmol / L, 2mmol / L, 3mmol / L, 4mmol / L, 5mmol / L and 6mmol / L) by streaking method. (sucrose yeast: 10.0g, yeast powder: 0.5g, K 2 HPO 4 : 0.5g, NaCl: 0.2g, MgSO 4 : 0.2g, CaCO 3 : 1.0g; pH: 7.0±0.2 to 1L) medium, cultivated in a 30°C incubator, observed and recorded the growth, the results are shown in Table 1, where "+" represents growth, "-" represents not grow.

[0055] Table 1 The growth of Paenibacillus colioids in different concentrations of glyphosate

[0056] Glyphosate concentration (mM) Growth of Paenibacillus glialis 1 + 2 + 3 + 4 － 5 － 6 －

Embodiment 2

[0057] The determination of embodiment 2 ultraviolet mutagen dosage

[0058] Inoculate the activated Paenibacillus colioids into a 150ml Erlenmeyer flask containing 50ml ACCC55 liquid medium and cultivate overnight, wash twice with an equal amount of 0.9% saline, and suspend with 0.9% saline, take 6ml suspension After irradiating for 0s, 6s, 12s, 18s, 24s and 30s with UV cross-linking instrument (energy: 1J / m2), spread the plates and record the number of colonies. The experimental results are shown in Table 2.

[0059] Table 2 The lethality of Paenibacillus coliformis under different ultraviolet irradiation time

[0060] time / s Bacteria count / cfu Fatality / % 0 12100 0 6 1200 90.08 12 170 98.6 18 0 100 24 0 100 30 0 100

Embodiment 3

[0061] Example 3 Ultraviolet Screening of Paenibacillus colioids Resistance to Isopropylamine Salt of Glyphosate

[0062] Take the time point when the lethality rate is 98.60%, repeat the process of activation-washing-ultraviolet mutagenesis, and get 200 μl of the bacterium liquid after the mutagenesis to inoculate in the ACCC55 solid medium containing 5.0mmol / L glyphosate isopropylamine salt, Cultured in an incubator at 30°C and observed the growth of the strains, initially screened 7 strains of Paenibacillus colioids that grew well, preserved them and carried out subsequent experiments. Inoculate 7 strains with resistance to 5mmol / L glyphosate isopropylamine salt into the ACCC55 liquid medium without glyphosate isopropylamine salt, and after cultivating for 12h, get the bacterial liquid and inoculate it into 5.0mmol / L glyphosate isopropylamine salt. In the ACCC55 solid medium of propylamine salt, cultivate at 30°C, and observe the growth. The results showed that only one st...

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Abstract

The invention provides a glyphosate-resistant Paenibacillus colloidus JD-07, bacterial agent and application, and relates to the field of microorganisms. Paenibacillus mucilaginosus (Paenibacillus mucilaginosus) JD‑07 has a preservation number in the General Microorganism Center of China Microbiological Culture Collection Management Committee: CGMCC No.17201. Phosphate isopropylamine salt growth medium, and compared with the wild strains of Paenibacillus coliformis, the morphological characteristics and physiological and biochemical properties were not changed. The glyphosate-resistant Paenibacillus colloidus JD‑07 and the bacterial agent containing it alleviate the problem that glyphosate causes the death of the Paenibacillus colioids in the product and cannot normally exert the efficacy of the Paenibacillus colioids.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a glyphosate-resistant Paenibacillus colloidus JD-07, bacterial agent and application. Background technique [0002] Glyphosate is a herbicide with high efficiency, low toxicity, broad-spectrum and systemic absorption, and non-selective foliar spraying, which is widely used in agricultural production (Chen Shiguo et al., 2017). However, while glyphosate provides convenience for agricultural production, it remains in large quantities in the soil. Researches on glyphosate at home and abroad show that, according to the normal application rate of 1kg / hm 2 According to calculations, the glyphosate residue in the surface 13cm soil can reach 0.45-2mg / kg; while in areas where glyphosate usage is relatively large, the residual amount can be as high as 10-20mg / kg (Zhou et al, 2013). These residual glyphosate not only caused great pollution to soil and water bodies, but also seriously hinder...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/80C05G3/20C05G3/00C05G3/60C05G3/30C05G3/40C05G3/70C05G3/90C05G3/50C05F11/08C09K17/14C12R1/01
CPCC05F11/08C05G3/00C09K17/14C12N1/20C12N1/205C12R2001/01
Inventor 刘海明杜蓉蓉吕中文
Owner 北京航天恒丰科技股份有限公司
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