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Calibration data correction method and electronic equipment thereof

A data correction and reaction data technology, applied in the field of data screening, can solve problems such as easy error fitting and inability to adapt to the environment with large noise, so as to ensure the accuracy and avoid the effect of curve error fitting

Active Publication Date: 2019-08-02
GENRUI BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have different limitations in practical applications, such as inability to adapt to a noisy environment or easy to wrongly fit abnormal data, etc.

Method used

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  • Calibration data correction method and electronic equipment thereof
  • Calibration data correction method and electronic equipment thereof
  • Calibration data correction method and electronic equipment thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0162] In this example 1, skipped Figure 6 The shown steps (S603 to S609) related to the initial screening directly perform curve fitting on the original data set. in, Figure 7 It is a schematic diagram of the raw data collection obtained by executing step S601 in Embodiment 1 of the present invention. Figure 8 To skip the initial screening, proceed directly to the Figure 7 Schematic diagram of the fitting results of curve fitting on the raw data set shown.

[0163] Such as Figure 7 and Figure 8 As shown, in Example 1, there are relatively many abnormal points that do not conform to the reaction trend, and the fitting curve mistakenly identifies many abnormal points that do not conform to the reaction law as normal points, resulting in the fitted curve not being able to reflect the correct reaction law , the calibration results are not ideal.

Embodiment 2

[0165] In this embodiment 2, also with Figure 7 Based on the raw data set shown, perform Figure 6 The primary screening steps (S603 to S609) and the secondary screening steps are shown to obtain the final fitting curve and corrected data set. in, Figure 9 Schematic diagram of the initial screening steps.

[0166] Such as Figure 9 As shown, through the straight line fitting method of the initial screening, it can be effectively identified Figure 7 Most of the abnormal points do not conform to the reaction law. The method of deleting these identified abnormal points and then performing curve fitting can well avoid the problem of mistakenly identifying abnormal points as normal points during curve fitting.

Embodiment 3

[0168] In this embodiment 3, execute Figure 6 In the data correction method shown, the original data set is screened and eliminated to obtain an accurate response curve and exclude interference factors.

[0169] in, Figure 10 It is a schematic diagram of the raw data collection obtained by executing step S601 in Embodiment 3 of the present invention. Figure 11 for initial screening Figure 10 Schematic diagram of the fitting results for the raw data set shown. Figure 12 A schematic diagram of the fitting results of the secondary screening.

[0170] Such as Figure 11 As shown, the initial screening first identified Figure 10 Most of the outliers in the original data set and deleted. Such as Figure 12 shown in Figure 11 On the basis of the fitting results, through the fitting curve with stronger fitting ability, it can provide a more accurate response curve that is more in line with the reaction trend.

[0171] From the comparison of Example 1 and Example 2, it ...

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Abstract

The embodiment of the invention relates to a calibration data correction method and electronic equipment thereof. The calibration data correction method comprises the following steps that: receiving an original dataset formed by multiple pieces of reaction data; through linear fitting, carrying out primary screening on the original dataset to obtain a primary-screening dataset; and through nonlinear fitting, carrying out secondary screening on the primary-screening dataset to obtain a corrected dataset. By use of the method, an original reaction curve dataset is subjected to coarse screening and fine screening in sequence, two fitting ways are used for iteratively removing and rejecting exceptional data which does not conform to a reaction tendency and a normal reaction rule, and the accuracy of a final calibration result is guaranteed.

Description

[0001] 【Technical field】 [0002] The invention relates to the technical field of data screening, in particular to a calibration data correction method and electronic equipment thereof. [0003] 【Background technique】 [0004] Nephelometric assay is a very widely used antigen / antibody quantitative assay. It utilizes that during the specific time period of the antigen-antibody reaction (that is, the combination between the sample antigen / antibody and the reagent antibody / antigen added in the cuvette to form a specific conjugate), the reaction speed is directly proportional to the concentration of the analyte , The increase or decrease of the scattered light intensity (absorbance) during this period of time is proportional to the concentration of the analyte, and the quantitative determination of the antigen / antibody is realized. [0005] Taking the measurement of scattered light intensity as an example, a light source can be used to irradiate the cuvette, and the scattered ligh...

Claims

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Application Information

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IPC IPC(8): G01N21/51G01N21/59
CPCG01N21/51G01N21/59
Inventor 林贵文
Owner GENRUI BIOTECH INC
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