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Method for identifying banana aquaporin gene promoter transcription factors

A technology of transcription factors and channel proteins, which is applied in the field of identification of transcription factors of the promoter of banana aquaporin gene, can solve problems such as methods for regulating AQP gene expression by transcription factors that have not been found

Pending Publication Date: 2019-08-30
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI +1
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Problems solved by technology

[0004] At present, in terms of revealing the molecular regulatory mechanism of MaPIP1;1 mediating the response of banana to drought stress in plants, there is no related method for transcription factors to regulate AQP gene expression

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  • Method for identifying banana aquaporin gene promoter transcription factors

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Embodiment Construction

[0022] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0023] refer to figure 1 , a method for identifying a banana aquaporin gene promoter transcription factor, comprising the following steps:

[0024] S1: Cloning and element analysis of the MaPIP1;1 promoter

[0025] The primers of the promoter were designed according to the banana A genome website to amplify, and the amplified length was 1362bp. The cis-acting elements were analyzed by plant care and PLACE, and the results showed that there were 72 cis-acting elements in the 1362bp promoter sequence, including many core elements of TATA box and CAAT box, including ABA response element ABRE, MYB element, MYC Elements, ERE elements, MeJA response elements, BOX II, G b...

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Abstract

The present invention discloses a method for identifying banana aquaporin gene promoter transcription factors. The method comprises the following steps: S1, cloning and element analysis of a MaPIP1;1promoter are conducted: amplification is conducted according to primers of existing genome designed promoters to an amplification length of 1,362 bp, cis-acting elements are analyzed by plant care andPLACE, and results show that there are 72 cis-acting elements in the 1,362 bp promoter sequence. A core region of the MaPIP1;1 promoter is recognized by a yeast one-hybrid method, the promoter core region M-P2 is used as a bait segment to construct a bait vector, an action mechanism of a MaPIP1;1 gene against drought stress is clarified, and the transcription factors directly binding with the MaPIP1;1 promoter under the drought stress condition are directly identified in plants for the first time.

Description

technical field [0001] The invention relates to the technical field of banana tolerance, in particular to a method for identifying a transcription factor of a banana aquaporin gene promoter. Background technique [0002] Banana is one of the important economic crops in the tropics, and is positioned by the Food and Agriculture Organization of the United Nations (FAO) as the fourth largest food crop in developing countries after rice, wheat, and corn. Banana is the fresh fruit with the largest fruit trade volume and consumption in the world. Banana is one of the fruits most affected by drought stress. Drought stress seriously restricts the production of banana, which is a key problem that needs to be solved urgently in the banana industry. Aquaporins are a class of small molecular transmembrane proteins located at 24-34Kd on various cell membranes, which can regulate the transportation of water and other small molecular substances, and play an important role in the process of...

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Application Information

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IPC IPC(8): C12Q1/34C12Q1/66C12Q1/6851
CPCC12Q1/34C12Q1/66C12Q1/6851C12Q2531/113C12Q2563/107
Inventor 许奕宋顺金志强徐碧玉李敬阳黄东梅王安邦李羽佳魏卿
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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