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Primer and probe composition for detecting obesity and type 2 diabetes, and application method thereof

A type 2 diabetes and composition technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of inconvenient detection, large sample size, and long time consumption, so as to avoid time-consuming Labor-intensive, simple operation and accurate results

Pending Publication Date: 2019-10-08
厦门蓝特生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Currently, only obesity or diabetes are tested, and the method can only indicate a certain risk
In addition, other sequencing-based methods require a large sample size and take a long time, making it inconvenient and flexible to carry out detection

Method used

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  • Primer and probe composition for detecting obesity and type 2 diabetes, and application method thereof
  • Primer and probe composition for detecting obesity and type 2 diabetes, and application method thereof

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Embodiment Construction

[0046] In order to enable those skilled in the art to better understand the present invention, the following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only a part of the present invention, rather than Full examples.

[0047] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention.

[0048] In order to achieve the above object, the embodiments of the present invention provide the following technical solutions, proposing a Q-PCR method to detect obesity and type 2 diabetes susceptibility gene mutation site amplification primers and probes, the obesity susceptibility gene to be detected The mutation sites are: rs4994 site on the ADRB3 gene, rs6265 site on the BDNF gene, rs7566605 site on the INSIG2 gene; rs9465871 site on the CDKAL1 gene, th...

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Abstract

The invention provides a primer and probe composition for detecting obesity and type 2 diabetes, which comprises a primer and a probe for the rs4994 site on a ADRbeta3 gene, and a primer and a probe for the rs9465871 site on a CDKAL1 gene. the primers and the probe targeting the rs4994 site of the ADRbeta3 gene include: an upstream primer of ADRbeta34994, a downstream primer of ADRbeta34994; and aprobe of ADRbeta34994; and the primer and the probe targeting the rs9465871 site on the CDKAL1 gene include: an upstream primer of CDKAL1; a downstream primer of CDKAL1; and a probe of CDKAL1. The invention provides a detection method for detecting obesity and type 2 diabetes-related mutation gene loci with convenient detection, high accuracy and economy; real-time quantitative PCR amplificationof a sample DNA is carried out by multiple sets of gene primers, and the genotype of the subject can be obtained by probe typing to achieve rapid detection of obesity and type 2 diabetes susceptibility gene mutation loci.

Description

technical field [0001] The invention relates to the field of molecular biology detection, in particular to amplification primers and probes for detecting mutation sites of obesity and type 2 diabetes susceptibility genes by Q-PCR method and application thereof. Background technique [0002] Obesity, as a common nutritional and metabolic disease, refers to excessive total body fat content and / or excessive local body fat content. It is a chronic metabolic disease mainly caused by the joint action of genetic and environmental factors. With the change of lifestyle, the prevalence of obesity is rising rapidly and has become a global public health problem. In 2002, WHO listed obesity as one of the global first-level risk factors causing the burden of human disease. At present, China has surpassed the United States to become the country with the largest number of obese people in the world. Therefore, preventing the epidemic of obesity will also be the biggest public concern facing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2563/107C12Q2531/113
Inventor 张帮周林爱强姜涛柯栋贤林晨怡杨璐溪
Owner 厦门蓝特生物科技有限公司
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