Method for selectively determining Cu < 2 + > by using one-step microwave hydrothermal synthesized fluorescent carbon dots

Abstract

The invention discloses a method for selectively determining Cu < 2 + > by using one-step microwave hydrothermal synthesized fluorescent carbon dots. The method is characterized by comprising the following steps: S1, preparing carbon dots; S2, separating and purifying the carbon dots; S3, concentrating a carbon dot solution; S4, adjusting the pH value of the carbon dot concentrated solution to 14,taking the carbon dot solution, scanning the emission spectrum of the carbon dot solution when an EX is in a range of 360-580nm, and detecting the fluorescence intensity; S5, concentrating a solutioncontaining the metal cations until the solution is close to a saturated state, pouring the concentrated solution containing metal cations into the carbon dot solution, scanning the emission spectrumof the carbon dot solution, detecting fluorescence intensity, determining the fluorescence of the carbon dot solution is obviously quenched, and considering the solution containing metal cations contains Cu < 2 + >. According to the invention, the new method for detecting Cu < 2 + > based on the fluorescence quenching mechanism is successfully established by using the carbon dot as a fluorescenceprobe, and the method has characteristics of simple operation, environmental protection, low cost and short required time, and provides the simple and rapid method for rapid and real-time Cu < 2 + > detection.

Description

technical field [0001] The invention belongs to fluorescent carbon dots for Cu 2+ The selective determination method technical field, be specifically related to the fluorescent carbon dot of one-step microwave hydrothermal synthesis for Cu 2+ selectivity measurement method. Background technique [0002] The human body cannot maintain normal physiological functions without the intake of various trace elements. Copper is an indispensable trace element in the human body. Medical research shows that when the human body lacks copper, the pigment oxidase in brain cells decreases. , Vitality decreases, which will cause memory loss, incoherent speech, slow response, insensitivity, and abnormalities. while excess Cu 2+ Can cause adverse effects on human health, such as liver or kidney damage, gastrointestinal discomfort [1] . So Cu 2+ It is a double-edged sword for human health, and it is very important to detect its content in biological systems with high selectivity and high ...

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Problems solved by technology

[0004] The purpose of the present invention is to provide one-step microwave hydrothermal synthesis of fluorescent carbon dots for Cu 2+ Selective determination method to solve the problem that when the existing copper ion selective determination method is used, the steps of the copper ion selective determination method are cumbersome, the production of carbon dots used for detection is low, and the process is long, which affects the copper ion selective determination method The problem of detection effect

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