Preparation method of controlled release hydrogel for tumor postoperative radiotherapy in combination with photothermal-photodynamic therapy

A combined therapy and photodynamic technology, which is applied in the direction of antineoplastic drugs, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problems of clean tumor resection and tumor recurrence, and achieve convenient storage and experimentation. The operation process is simple and the effect of improving stability

Inactive Publication Date: 2019-10-22
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the treatment of tumors in our country is still based on surgical resection, but most of the operations cannot completely remove the tumor, which leads to tumor recurrence.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Take 1g silk, with 0.5% Na 2 CO 3 Under the condition that the bath ratio is 1:100, the silk is degummed twice in a water bath at 90°C, each time for 30 minutes, and rinsed three times with deionized water after each degumming to remove residual impurities;

[0034] 2) Prepare 500mL of 9.3M lithium bromide solution, and dissolve the silk in a water bath at 60°C under the condition of a bath ratio of 1:50 for 3 hours;

[0035] 3) Place the solution in step 2) at 4°C, and dialyze in deionized water in an 8000Da dialysis bag for 2 days;

[0036] 4) Dialyze the dialyzed solution in step 3) in a 8000Da dialysis bag at 4°C for 2 days in PBS solution;

[0037] 5) Concentrate the solution obtained in step 4) in an 8000Da dialysis bag in 20wt% polyethylene glycol at 4°C to obtain a 6wt% silk fibroin solution, which is sealed and placed in an environment of 1°C refrigerated for later use;

[0038] 6) Dissolve 0.5g of melanin in 10mL of deionized water, then take 1g of CuCl ...

Embodiment 2

[0046] 1) Take 1g silk, with 0.5% Na 2 CO 3 Under the condition that the bath ratio is 1:100, the silk is degummed twice in a water bath at 95° C. for 35 minutes each time, and rinsed three times with deionized water after each degumming to remove residual impurities;

[0047] 2) Prepare 500mL of 9.3M lithium bromide solution, and dissolve silk in a water bath at 60°C for 3.5 hours at a bath ratio of 1:50;

[0048] 3) Place the solution in step 2) at 4°C, and dialyze in deionized water in an 8000Da dialysis bag for 2 days;

[0049] 4) Put the dialyzed solution in step 3) in an 8000Da dialysis bag at 4°C and dialyze in PBS solution for 3 days;

[0050] 5) Concentrate the solution obtained in step 4) in an 8000Da dialysis bag at 4°C in 20wt% polyethylene glycol to obtain a 6wt% silk fibroin solution, which is sealed and placed in an environment at 4°C refrigerated for later use;

[0051] 6) Dissolve 0.5g of melanin in 10mL of deionized water, then take 1.25g of CuCl 2 2H 2...

Embodiment 3

[0059] 1) Take 1g silk, with 0.5% Na 2 CO 3 Under the condition that the bath ratio is 1:100, the silk is degummed twice in a water bath at 100°C, each time for 40 minutes, and rinsed three times with deionized water after each degumming to remove residual impurities;

[0060] 2) Prepare 500mL of 9.3M lithium bromide solution, and dissolve the silk in a water bath at 60°C under the condition of a bath ratio of 1:50 for 4 hours;

[0061] 3) Place the solution in step 2) at 4°C, and dialyze in an 8000Da dialysis bag in deionized water for 3 days;

[0062] 4) Put the dialyzed solution in step 3) in an 8000Da dialysis bag at 4°C and dialyze in PBS solution for 3 days;

[0063] 5) Concentrate the solution obtained in step 4) in an 8000Da dialysis bag in 20wt% polyethylene glycol at 4°C to obtain a 6wt% silk fibroin solution, which is sealed and placed in an environment of 5°C refrigerated for later use;

[0064] 6) Dissolve 0.5g of melanin in 10mL of deionized water, then take ...

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Abstract

The invention relates to the field of biomedical polymer materials and discloses a preparation method of a controlled release hydrogel for tumor postoperative radiotherapy in combination with photothermal-photodynamic therapy. The hydrogel which has a controlled release function and is used for the tumor postoperative radiotherapy in combination with the photothermal-photodynamic therapy is obtained by adopting silk fibroin loaded with copper sulfide@melanin-PEG@dopamine nanoparticles and a photosensitizer 5-aminolevulinic acid, wherein the silk fibroin has good biocompatibility, and the copper sulfide@melanin-PEG@dopamine nanoparticles have a great photothermal property and a good radiotherapy function. The hydrogel can be used for performing photothermal therapy, photodynamic therapy andradiation therapy on a postoperative tumor part. At the same time, the hydrogel has a good controlled release property and can achieve the effects of reducing the drug delivery dosage and reducing oravoiding toxic and side effects. The stability of drugs is improved, and the hydrogel is convenient to store; some new drug delivery routes can be established.

Description

technical field [0001] The invention relates to the field of biomedical polymer materials, in particular to a method for preparing a controlled-release hydrogel used in postoperative radiotherapy photothermal-photodynamic combined therapy for tumors. Background technique [0002] At present, the treatment of tumors in my country is still dominated by surgical resection, but most operations cannot completely remove the tumor, which leads to tumor recurrence. After surgical resection of the tumor, in order to remove the remaining tumor tissue, we need a treatment method that can act in a small local area and with milder conditions to achieve this goal. Photothermal therapy and photodynamic therapy, which can target specific areas and have mild conditions, are undoubtedly more suitable options. At the same time, slow postoperative wound healing is also one of the problems that needs to be solved urgently in this field. [0003] As a new type of biomedical material, hydrogel ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/64A61K47/60A61K41/00A61P35/00
CPCA61K41/0038A61K41/0052A61K41/0061A61K47/60A61K47/64A61K47/6903A61P35/00A61K2300/00
Inventor 王秉金小康邱方燚蒋鹏胡智文
Owner ZHEJIANG SCI-TECH UNIV
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