Polypeptide sequence and preparation method and application of self-assembling material of polypeptide sequence

A technology for polypeptide sequence and self-assembly, applied in the field of its preparation and self-assembly of polypeptides, can solve problems such as pollution, and achieve the effects of low cost, convenient wide application and high stability

Active Publication Date: 2019-11-05
SOUTHWEST MEDICAL UNIVERISTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, heavy metals discharged with wastewater, even if the concentration is small, can also accumulate in algae and sediment, be absorbed by the body surface of fish and shellfish, produce food chain concentration, and cause public nuisance

Method used

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  • Polypeptide sequence and preparation method and application of self-assembling material of polypeptide sequence
  • Polypeptide sequence and preparation method and application of self-assembling material of polypeptide sequence
  • Polypeptide sequence and preparation method and application of self-assembling material of polypeptide sequence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0037] Example 2: The polypeptide freeze-dried powder is dissolved in deionized water to configure a 200 μg / ml solution, the polypeptide is a soluble polypeptide, and the polypeptide solution is colorless and transparent. Take 5ml of the solution for circular dichroism detection. And calculate the percentage of various structures in the secondary structure of the polypeptide based on the detection results.

[0038] Circular dichroism (CD for short) is the most widely used method for determining the secondary structure of proteins, and it is a fast, simple and accurate method for studying protein conformation in dilute solutions.

Embodiment 3

[0039] Example 3: The polypeptide described herein was formulated into a 200 μg / ml solution, and the solution was colorless and transparent. Adding the disodium hydrogen phosphate that final concentration is 0.1M in the solution, visible white granular substance, the minimum concentration of disodium hydrogen phosphate that can form white granule is 5mM after testing. Aspirate 50 μl of the solution and place it on a glass slide, and take a cover glass to cover the droplet. Observe the results under an inverted microscope.

Embodiment 4

[0040] Example 4: The polypeptide described herein was formulated into a 200 μg / ml solution, and the solution was colorless and transparent. Disodium hydrogen phosphate was added to the solution to a final concentration of 0.1M. The sample is dropped on the copper sheet, and after drying, the copper sheet loaded with the sample is stuck on the conductive tape on the sample holder. Step by step for photo shooting.

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Abstract

The invention discloses a synthetic polypeptide. According to the synthetic polypeptide, (a), an amino acid sequence of the synthetic polypeptide is shown as SEQ ID No.1 and 2; and (b), a polypeptidederived from (a) and with the same biological function as the polypeptide in (a) are prepared by deleting, inserting or replacing one or several amino acids in the synthetic polypeptide limited by (a). The synthetic polypeptide is a synthetic polypeptide with hydrogen phosphate-mediated self-assembly ability, and a prepared self-assembling substance has a cationic affinity activity, and metal ionsin an aqueous solution can be enriched. A recombinant strain of a polypeptide sequence has inorganic phosphate and enriching activity of and metallic nickel ions after escherichia coli is subjected to recombinant induced expression. The synthetic polypeptide is non-toxic and has certain application potential.

Description

Technical field: [0001] The invention belongs to the field of biological materials, and relates to a self-assembled polypeptide, its preparation method and application. Background technique: [0002] Self-assembly refers to a technique in which basic structural units spontaneously form ordered structures. In the process of self-assembly, the basic structural units spontaneously organize or aggregate into a stable structure with a certain regular geometric appearance under the interaction based on non-covalent bonds. Self-assembled polypeptides are the most common and important ones. Most of the natural gels in organisms and many synthetic polymer gels are self-assembled polypeptides. As a kind of soft material, self-assembled polypeptide has a wide range of applications in many aspects, such as contact lenses, artificial muscles, cell separation and culture, material extraction, immobilized enzymes, controlled release of drugs, etc. There are two main types of self-assembl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/02B01J20/26B01J20/30C02F1/28C02F101/20
CPCC07K1/02B01J20/26C02F1/285C02F2101/20
Inventor 张志坤周英顺
Owner SOUTHWEST MEDICAL UNIVERISTY
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