Trace sperm freezing and thawing method and automatic clamping apparatus thereof

A jig device, sperm technology, applied to the preservation of animal cells, vertebrate cells, human or animal bodies, etc., to achieve the effects of uniform fumigation, improved flexibility, and convenient operation

Inactive Publication Date: 2019-11-12
李达
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of microsperm freezing and thawing method a

Method used

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  • Trace sperm freezing and thawing method and automatic clamping apparatus thereof
  • Trace sperm freezing and thawing method and automatic clamping apparatus thereof
  • Trace sperm freezing and thawing method and automatic clamping apparatus thereof

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Embodiment

[0031] The present invention provides such Figure 1-5 A kind of microsperm freezing and thawing method shown, comprises the following steps:

[0032] S1. Freezing of sperm: First, after optimizing the enrichment of semen, the sperm and cryoprotectant are mixed evenly at a ratio of 1:1 to obtain frozen droplets and placed at room temperature for later use. Liquid nitrogen is injected into the liquid nitrogen box to make the liquid nitrogen The injection volume is one-half of the volume of the liquid nitrogen box, and the automatic clamping device is used to clamp the frozen load rod. The frozen load rod is 20cm away from the liquid ammonia vapor surface, and the mixed frozen liquid drops are dropped in an amount of 5-10uL on the The slices of the freezing rods are lowered to the position of 5 cm above the liquid nitrogen surface at a speed of 5 cm / min by the automatic clamp device, and then the freezing rods are lowered into the liquid nitrogen at a speed of 1 cm / min. After th...

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PUM

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Abstract

The invention discloses a trace sperm freezing and thawing method and an automatic clamping apparatus thereof. The method comprises: S1, freezing sperms; and S2, thawing the sperms, wherein a fixing frame is placed on the bottom portion of the inner side of a liquid nitrogen box, a lifting motor is fixed on the top portion of the fixing frame, and the output shaft of the lifting motor is fixedly connected to a lifting screw rod penetrating through the fixing frame. According to the present invention, the apparatus can simultaneously freeze a plurality of freezing carrying rods, such that the operation is convenient, efficient and fast, and the sperm micro-droplets on the freezing carrying rods are subjected to uniform and accurate fumigation by the condenser so as o substantially improve the freezing resuscitation rate of the trace sperms and reduce the mistakes and the frostbite of the operator; and with the method and the apparatus, the problems that the sperm recovery is easy to fail after the sperms of paints with oligospermatism and asthenospermia, the testis sperms and the epididymal sperms are frozen in the current sperm freezing technology is well solved so as to substantially improve the assisted reproductive therapy flexibility on patients with azoospermia.

Description

technical field [0001] The invention belongs to the field of human assisted reproduction, and in particular relates to a micro-sperm freezing and thawing method and an automatic clamp device thereof. Background technique [0002] Environmental pollution is becoming more and more serious, hormone abuse, etc. lead to a continuous decline in the quality and quantity of male sperm, and the proportion of male infertility is increasing day by day. Worldwide, infertility caused by male infertility accounts for about 40-50%, while azoospermia is about It accounts for 10% of male infertility problems, affects 1% of the male population in the world, and the incidence of azoospermia will gradually increase. [0003] In the past, patients with azoospermia could not have their own genetic children, but with the development of assisted reproductive technology, patients with azoospermia can undergo percutaneous epididymal sperm aspiration (PESA), orchiectomy ( TESA), micro-orchiectomy spe...

Claims

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Application Information

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IPC IPC(8): A01N1/02C12N5/076
CPCA01N1/0257C12N5/061
Inventor 李娜苗聪秀李敏
Owner 李达
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