Fermentation method for increasing content of abamectin B1a
A fermentation method and abamectin technology are applied in the directions of microorganism-based methods, fermentation, biochemical equipment and methods, etc., to achieve the effects of improving oxygen solubility, reducing oxygen transfer resistance, and simple process
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Embodiment 1
[0032] Carry out in parallel with Comparative Example 1, other conditions are the same as Comparative Example 1, the fermenter fermentation adopts the oxygen carrier addition strategy, when the fermentation is carried out to the 12th hour, add 7% of the volume of the fermented liquid in the fermenter. alkanes until the end of fermentation, the fermentation titer was 7513µ / ml, and the B1a content was 97.2%.
Embodiment 2
[0034] Carry out in parallel with comparative example 2, other conditions are the same as comparative example 2, fermentor fermentation adopts oxygen carrier addition strategy, when fermentation carries out to the 24th hour, add the oxygen carrier positive of 5% of fermented liquid volume in fermentor Dodecane until the end of fermentation, the fermentation titer was 7698µ / ml, and the B1a content was 97.6%.
Embodiment 3
[0036] Carry out in parallel with comparative example 3, other conditions are the same as comparative example 3, fermentor fermentation adopts oxygen carrier addition strategy, when fermentation carries out to the 48th hour, add the oxygen carrier positive of 3% of fermented liquid volume in fermentor Dodecane until the end of fermentation, the fermentation titer was 7362µ / ml, and the B1a content was 96.5%.
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