Fermentation method for increasing content of abamectin B1a

A fermentation method and abamectin technology are applied in the directions of microorganism-based methods, fermentation, biochemical equipment and methods, etc., to achieve the effects of improving oxygen solubility, reducing oxygen transfer resistance, and simple process

Pending Publication Date: 2019-12-10
NINGXIA TAIYICIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using the addition of oxygen carrier to optimize the fermentation process of abamectin and increase the content of abamectin B1a has not yet been reported in the literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Carry out in parallel with Comparative Example 1, other conditions are the same as Comparative Example 1, the fermenter fermentation adopts the oxygen carrier addition strategy, when the fermentation is carried out to the 12th hour, add 7% of the volume of the fermented liquid in the fermenter. alkanes until the end of fermentation, the fermentation titer was 7513µ / ml, and the B1a content was 97.2%.

Embodiment 2

[0034] Carry out in parallel with comparative example 2, other conditions are the same as comparative example 2, fermentor fermentation adopts oxygen carrier addition strategy, when fermentation carries out to the 24th hour, add the oxygen carrier positive of 5% of fermented liquid volume in fermentor Dodecane until the end of fermentation, the fermentation titer was 7698µ / ml, and the B1a content was 97.6%.

Embodiment 3

[0036] Carry out in parallel with comparative example 3, other conditions are the same as comparative example 3, fermentor fermentation adopts oxygen carrier addition strategy, when fermentation carries out to the 48th hour, add the oxygen carrier positive of 3% of fermented liquid volume in fermentor Dodecane until the end of fermentation, the fermentation titer was 7362µ / ml, and the B1a content was 96.5%.

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PUM

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Abstract

The invention relates to a fermentation method for increasing the content of abamectin B1a. The fermentation method comprises the step: carrying out seed culture and fermentation culture on streptomyces avermitilis serving as a fermentation strain. The fermentation method is characterized in that an oxygen carrier is added in a fermentation culture process. The oxygen carrier and a fermentation solution are not mutually dissolved by adding the oxygen carrier n-dodecane not serving as a carbon source when the abamectin is fermented for 12-20 h, so that the direct oxygen transfer resistance of agas phase and a liquid phase is reduced to the great extent, the oxygen dissolution capacity of a substrate is improved, the fermentation potency of the abamectin can be kept at 7000 mu / ml-8000 mu / ml, and the content of the abamectin B1a can be 96%-98%. The fermentation method is simple and effective in process, can be applied to industrial production of abamectin and has a certain guiding significance for the optimization of an abamectin fermentation process.

Description

technical field [0001] The invention relates to a production process of avermectin, a fermentation product of Streptomyces avermitilis, and belongs to the field of antibiotic fermentation, in particular to a fermentation process for increasing the content of abamectin B1a. Background technique [0002] Abamectin is a commonly used pesticide in my country, and it has the effect of repelling and killing nematodes, insects, and mites. The commonly used method for optimizing the fermentation of abamectin is mainly to improve the control process in the fermentation process, such as feeding, precursor feeding, pH control, etc., by optimizing the medium formula. In fact, during the fermentation process, the dissolved oxygen concentration in the fermentation broth can directly affect the activity of enzymes and metabolic pathways of microorganisms, and have an important impact on the growth of microorganisms and product synthesis. [0003] Traditional methods to increase the dissol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62C12R1/465
CPCC12P19/623
Inventor 刘鹏张杭洲徐雪风郭佳王少云
Owner NINGXIA TAIYICIN BIOTECH CO LTD
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