Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

MiRNAs related to highland barley stripe disease and excavation method thereof

A stripe and highland barley technology, applied in the field of miRNAs and their mining, can solve the problems of less research and other issues

Pending Publication Date: 2019-12-13
QINGHAI UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current reports of plant disease-related miRNAs mainly come from Arabidopsis, and there are relatively few studies in monocotyledonous and wheat cereal crops.
No miRNAs have been reported in barley, and only 71 miRNAs have been recorded even in barley

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • MiRNAs related to highland barley stripe disease and excavation method thereof
  • MiRNAs related to highland barley stripe disease and excavation method thereof
  • MiRNAs related to highland barley stripe disease and excavation method thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment

[0055] (1) Investigation and sampling of field stripe disease:

[0056] The incidence of barley stripe disease was investigated in Qinghai Province for three consecutive years, and the barley stripe disease resistant variety Kunlun 14 and the susceptible variety ZDM6006 (Table 1) were obtained. The susceptibility and disease resistance of the susceptible and resistant highland barley varieties in the middle of the field Uninfected leaves were collected and stored in an ultra-low temperature refrigerator at -80 degrees.

[0057] Table 1 The incidence of stripe disease in the main cultivated barley varieties and the susceptible variety ZDM6006 in Qinghai Province

[0058]

[0059] (2) Construction of the pathogen infection system of stripe disease in laboratory

[0060] ①Isolation of pathogenic bacteria of barley stripe disease: The barley stripe diseased leaves in the middle stage of onset stored in the -80 degree ultra-low temperature refrigerator were rinsed with distilled water, and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides miRNAs (micro Ribonucleic Acids) related to highland barley stripe disease and an excavation method thereof. The method comprises the following steps: separating and purifying highland barley stripe bacterial strains from highland barley stripe disease leaves; constructing a laboratory stripe disease pathogenic bacteria infection system; collecting diseased and non-diseasedyoung leaves, carrying out high-throughput sequencing (miRNA-seq), constructing a database of highland barley miRNA and comparing the database with miRBase 22, PMRD, mRNA / EST and rRNA databases so asto unearth the miRNAs related to the highland barley stripe disease.

Description

Technical field [0001] The present invention relates to barley stripe disease, in particular to a kind of miRNAs related to barley stripe disease and a mining method thereof. Background technique [0002] Highland barley (Hordeum vulgare L.var.nudum) belongs to the genus Hordeum graminea. It is a variety of barley (Hordeumvulgare L.). Its genetic background is highly similar to that of barley. The inner and outer glumes are separated from the caryopsis and the grains are bare, so it is also called For naked barley. Highland barley is the most distinctive crop on the Qinghai-Tibet Plateau, and its production status plays an important role. [0003] Barley stripe disease is the most serious fungal disease affecting the production of highland barley ( figure 1 ). At present, the main method to prevent barley stripe disease is drug soaking treatment. However, the existing seed soaking medicines such as Li Kexiu, Fenpinning, Soaking Ling, etc. (active ingredients: tebuconazole, triad...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6809C12N15/113G16B50/30G16B40/00G16B30/10
CPCC12N15/113C12N2320/10C12Q1/6809G16B30/10G16B40/00G16B50/30C12Q2535/122C12Q2539/113
Inventor 姚晓华吴昆仑姚有华王越苏乐平侯璐白羿雄
Owner QINGHAI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com