Sebacinales sp. LQcapable of promoting germination of seeds of medicinal Dendrobium officinale andDendrobium flexicauleto form seedlings and application thereof

Abstract

The invention discloses Sebacinales sp. LQ capable of promoting germination of seeds of medicinal Dendrobium officinale andDendrobium flexicauleto form seedlings; and the Sebacinales sp. LQ is preserved in China Center for Type Culture Collection under the preservation number of CCTCC NO: M 2019744. The invention further provides morphological, physiological and biochemical characteristics of theSebacinales sp. LQ. According to the morphological, physiological and biochemical characteristics, as well as nucleotide sequence and phylogenetic analysis results, the Sebacinales sp. LQ. is a fungusstrain pertaining to the Sebacinales sp.. The Sebacinales sp. LQ. can be used for promoting germination of seeds of Dendrobium officinale and seeds of Dendrobium flexicaule to form seedlings; and theSebacinales sp. LQ. has relatively high specificity. Thus, the seeds of the Dendrobium officinale and the seeds of the Dendrobium flexicaule can be separatelyinoculated with the Sebacinales sp. LQ. so as to obtain high-quality symbiotic seedlings, thereby realizing efficient cultivation of seedlings by symbiotic germination; and moreover, symbiosis can be used for seedling raising in nursery or direct seed sowing on trunks in the field. Compared with tissue culture, symbiotic culture has the advantages of being low in cost, high in survival rate in the field, free of the problem of tissue degradation and so on; and thus, the symbiotic culture has application values in obtaining of symbiotic seedlings in replacement of tissue culture.

Description

technical field

[0001] The invention relates to a fungus fungus cereus for promoting the germination of medicinal dendrobium seeds to form seedlings and an application thereof, which belongs to the field of applied microorganisms. Background technique

[0002] Dendrobium officinale (Dendrobium officinale) and Dendrobium flexicaule (Dendrobium flexicaule), as traditional precious Chinese medicinal materials in my country, have extremely high medicinal value and have been used for more than 2,000 years. Reviving Grass". In the past, wild dendrobium was mainly collected. Due to the depletion of wild resources, the dendrobium industry has been dominated by intensive cultivation in greenhouses since the 1990s. After nearly 30 years of development, such an industrial model has huge investment in infrastructure and high requirements for planting technology. , Product safety and authenticity have been questioned. At present, the entire medicinal Dendrobium industry is in a transiti...

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