Sebacinales sp. LQcapable of promoting germination of seeds of medicinal Dendrobium officinale andDendrobium flexicauleto form seedlings and application thereof

A seed and strain technology, applied in the field of applied microorganisms, can solve the complex and cumbersome problems of effective fungal screening and separation, and achieve the effect of simple and convenient separation method, simple process and low cost

Active Publication Date: 2020-01-17
YUNNAN UNIV
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  • Description
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AI Technical Summary

Problems solved by technology

At present, effective fungi are mostly obtained by isolating from the roots of wild plants of Orchidaceae, but because there are a large number of endophytic fun

Method used

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  • Sebacinales sp. LQcapable of promoting germination of seeds of medicinal Dendrobium officinale andDendrobium flexicauleto form seedlings and application thereof
  • Sebacinales sp. LQcapable of promoting germination of seeds of medicinal Dendrobium officinale andDendrobium flexicauleto form seedlings and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Isolation of Sebacinales sp. LQ strain

[0039] (1) Acquisition of protocorms: In September 2018, 24 protocorms of Dendrobium candidum and 3 protocorms of Dendrobium curved stems that germinated naturally were collected in the original habitats of Dendrobium candidum and Dendrobium curved stem in Luotian, Chongqing, and wrapped with moist moss bring back;

[0040] (2) Induction, separation, purification and preservation of symbiotic fungi in protocorms: take out the germinated protocorms of Dendrobium officinale and / or protocorms of Dendrobium curved stems, put them into sterilized beakers filled with 1% sodium hypochlorite (NaClO) solution respectively, Gently shake the beaker, take it out with sterilized blunt-tipped tweezers after 5 minutes, and rinse it with sterile water for 3 to 4 times; cut the protocorm of Dendrobium candidum and / or the protocorm of Dendrobium candidum with a sterile blade on an ultra-clean workbench. Cut into two halves, place them ...

Embodiment 2

[0047] Embodiment 2: Experiment of the effectiveness of promoting the symbiotic germination of Dendrobium candidum LQ bacterial strain:

[0048] The symbiotic germination experiment between seeds and fungi in the medium was used to detect whether the isolated fungi had a promoting effect on the seed germination stage and to compare the differences in the promoting effects of different fungi on the seed germination stage:

[0049] (1) Prepare symbiotic germination medium: the symbiotic germination medium is oat agar medium, including 4g L -1 Oat flour and 8g·L -1 Agar, the pH of the medium is 5.6-5.8; prepare 360 ​​plates of oat medium for sterilization, and there are 6 treatment groups in total;

[0050] (2) Take out the Sebacinales sp.LQ which is stored in the slant of the test tube at a temperature of 4°C, inoculate it on the PDA medium, then place it in an artificial climate box, and cultivate it at a temperature of 25±2°C Activation until the fungal hyphae cover the petr...

Embodiment 3

[0059] Embodiment 3: Experiment of the effectiveness of promoting the symbiotic germination of Dendrobium bentrobii by the LQ strain of O. cereus:

[0060] The symbiotic germination experiment between seeds and fungi in the medium was used to detect whether the isolated fungi had a promoting effect on the seed germination stage and to compare the differences in the promoting effects of different fungi on the seed germination stage:

[0061] (1) Prepare symbiotic germination medium: the symbiotic germination medium is oat agar medium, including 4g L -1 Oat flour and 8g·L -1 Agar, the pH of the medium is 5.6-5.8; prepare 360 ​​plates of oat medium for sterilization, and prepare 30 plates for each experiment, 12 treatments in total;

[0062] (2) Take out the Sebacinales sp.LQ which is stored in the slant of the test tube at a temperature of 4°C, inoculate it on the PDA medium, then place it in an artificial climate box, and cultivate it at a temperature of 25±2°C Activation unt...

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Abstract

The invention discloses Sebacinales sp. LQ capable of promoting germination of seeds of medicinal Dendrobium officinale andDendrobium flexicauleto form seedlings; and the Sebacinales sp. LQ is preserved in China Center for Type Culture Collection under the preservation number of CCTCC NO: M 2019744. The invention further provides morphological, physiological and biochemical characteristics of theSebacinales sp. LQ. According to the morphological, physiological and biochemical characteristics, as well as nucleotide sequence and phylogenetic analysis results, the Sebacinales sp. LQ. is a fungusstrain pertaining to the Sebacinales sp.. The Sebacinales sp. LQ. can be used for promoting germination of seeds of Dendrobium officinale and seeds of Dendrobium flexicaule to form seedlings; and theSebacinales sp. LQ. has relatively high specificity. Thus, the seeds of the Dendrobium officinale and the seeds of the Dendrobium flexicaule can be separatelyinoculated with the Sebacinales sp. LQ. so as to obtain high-quality symbiotic seedlings, thereby realizing efficient cultivation of seedlings by symbiotic germination; and moreover, symbiosis can be used for seedling raising in nursery or direct seed sowing on trunks in the field. Compared with tissue culture, symbiotic culture has the advantages of being low in cost, high in survival rate in the field, free of the problem of tissue degradation and so on; and thus, the symbiotic culture has application values in obtaining of symbiotic seedlings in replacement of tissue culture.

Description

technical field [0001] The invention relates to a fungus fungus cereus for promoting the germination of medicinal dendrobium seeds to form seedlings and an application thereof, which belongs to the field of applied microorganisms. Background technique [0002] Dendrobium officinale (Dendrobium officinale) and Dendrobium flexicaule (Dendrobium flexicaule), as traditional precious Chinese medicinal materials in my country, have extremely high medicinal value and have been used for more than 2,000 years. Reviving Grass". In the past, wild dendrobium was mainly collected. Due to the depletion of wild resources, the dendrobium industry has been dominated by intensive cultivation in greenhouses since the 1990s. After nearly 30 years of development, such an industrial model has huge investment in infrastructure and high requirements for planting technology. , Product safety and authenticity have been questioned. At present, the entire medicinal Dendrobium industry is in a transiti...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G22/25C12R1/645
CPCA01G22/25C12N1/145C12R2001/645
Inventor 高江云王新菊
Owner YUNNAN UNIV
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