Automatic sampling instrument of single cells

An automatic sampling and single-cell technology, applied in the direction of sampling devices, etc., can solve the problems of the biological activity of the cut sample area, unfavorable research, burn samples, etc., and achieve the effect of controllable cutting position and size, good willfulness and low cost

Pending Publication Date: 2020-01-31
JIANGSU RAYME BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current single-cell sampling equipment at the tissue level mainly relies on high-power laser cutting technology, but the cost of this equipment is extremely high, especially the high-power laser beam will burn the edge of the sample, and at the same time, due to high temperature operation, the biological activity of the cut sample area will also be affected. Greatly affected, very unfavorable for later research

Method used

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  • Automatic sampling instrument of single cells
  • Automatic sampling instrument of single cells
  • Automatic sampling instrument of single cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0021] Experimental material: The sample is a fresh mouse brain slice (thickness is about 45um, size 15´12mm);

[0022] CCD1 magnification: 40 times;

[0023] Motor 5: light noise, small vibration, minimum speed 120rpm;

[0024] Mobile CCD adjustment bracket 3, cutting probe adjustment bracket 6: minimum precision 10um;

[0025] Hollow cutting probe 4: inner diameter 1000um, outer diameter 1500um;

[0026] High-definition imaging CCD 1: can realize online imaging function;

[0027] Sample heating stage 8: the temperature is 25 degrees Celsius, and the sample is placed in the middle position.

[0028] experiment procedure:

[0029] The tissue sample slices are placed in the center of the sample heating platform 8, and under the guidance of the high-definition imaging CCD 1, the position to be cut is marked;

[0030] Position adjustment: precisely adjust the hollow cutting probe 4 to the marked area by moving the CCD adjustment bracket 3 and the cutting probe adjustment fra...

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PUM

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Abstract

The invention discloses an automatic sampling instrument of single cells. The instrument comprises an upright imaging system, a microdissection system, a sample table system and a sample extraction system; the upright imaging system comprises a high-definition imaging CCD and a display which are arranged on a CCD adjusting bracket; the microdissection system comprises a hollow cutting needle head,a cutting probe adjusting frame, and a high-speed motor connected with the hollow cutting needle head; the hollow cutting needle head and the high-speed motor are arranged on the cutting probe adjusting frame; the sample table system comprises a sample table and a sample heating table arranged on the sample table; a sample sheet is arranged on the sample heating table; the hollow cutting needle head is aligned to the position over a to-be-cut area of the sample sheet through the cutting probe adjusting frame; the high-definition imaging CCD is adjusted through the CCD adjusting bracket to image the to-be-cut area on the display; and the sample extraction system comprises a suction pipe connected with the hollow cutting needle head and a vacuum pump connected with the suction pipe. A graduated scale is arranged on the sample heating table. The instrument has the advantages of precise cutting of tiny areas, no influence on the activity of cells and tissues in a cutting area, simplicityand convenience in operation, high cutting efficiency, low use cost and the like.

Description

technical field [0001] The invention relates to a single-cell automatic sampler, which belongs to the application of precision automation and image recognition technology in the technical field of biological detection. Background technique [0002] Single cell separation is an important sample pretreatment technology for single cell research, and directly obtaining single cell or micro-region samples from tissues or living bodies is closer to the actual living environment of cells, which has positive significance for the fields of clinical, biological, and pharmacy. Micro-area sampling at the tissue or living body level has extremely high requirements for the accuracy of sampling equipment due to the particularity of the sample. The current single-cell sampling equipment at the tissue level mainly relies on high-power laser cutting technology, but the cost of this equipment is extremely high, especially the high-power laser beam will burn the edge of the sample, and at the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/08
CPCG01N1/08
Inventor 徐艇
Owner JIANGSU RAYME BIOTECH
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