Specific CAPS molecular marker snp545 for identifying hypoallergenic specific soybean variety, method and application thereof

A molecular marker, hypoallergenic technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial assay/inspection, etc., can solve the problem of CAPS molecular markers, and achieve stable and reliable results.

Active Publication Date: 2020-04-03
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the CAPS molecular marker for soybean α-subunit deletion gene

Method used

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  • Specific CAPS molecular marker snp545 for identifying hypoallergenic specific soybean variety, method and application thereof
  • Specific CAPS molecular marker snp545 for identifying hypoallergenic specific soybean variety, method and application thereof
  • Specific CAPS molecular marker snp545 for identifying hypoallergenic specific soybean variety, method and application thereof

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Experimental program
Comparison scheme
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specific Embodiment approach 1

[0033] Specific embodiment 1: A soybean variety-specific CAPS molecular marker snp545 for identifying hypoallergenic special use according to this embodiment, the nucleotide sequence of the CAPS molecular marker is shown in SEQ ID NO:1.

specific Embodiment approach 2

[0034]Embodiment 2: The application of the specific CAPS molecular marker snp545 of this embodiment is used to identify low-allergenic special-purpose soybean varieties, and the low-allergenic special-purpose soybean varieties are soybean α-subunit-deleted varieties.

specific Embodiment approach 3

[0035] Specific embodiment three: a primer set for detecting the specific CAPS molecular marker snp545 according to this embodiment, the sequence of the primer set is shown in SEQ ID NO:2.

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PUM

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Abstract

The invention discloses a specific CAPS molecular marker snp545 for identifying hypoallergenic specific soybean variety, as well as a method and an application thereof, which relate to the field of molecular markers, and provides the specific CAPS molecular marker for identifying the hypoallergenic specific soybean variety, the method and the application thereof, and the nucleotide sequence of theCAPS molecular marker is shown as SEQ ID NO: 1. The snp545 marker disclosed by the invention is used for identifying the hypoallergenic specific soybean variety, and the hypoallergenic specific soybean variety is an alpha-subunit deletion variety, so that a good research basis is provided for excavation and identification analysis of alpha-subunit deletion candidate genes. Through multiple tests,the result is stable and reliable. The method is applied to the field of low-sensitivity special soybean breeding.

Description

technical field [0001] The invention relates to the field of molecular markers, in particular to a CAPS molecular marker and method for identifying low-allergenic special soybean varieties and its application. Background technique [0002] Allergens in soybean seeds mainly include storage proteins, structural proteins and defense-related proteins, and the main allergens are storage proteins. Storage proteins are classified according to the sedimentation coefficient during density gradient centrifugation, including 7S globulin, 11S globulin, 15S globulin, and 2S globulin. As early as 1934, Duke and other scholars pointed out that soybean is an important food allergen. As of April 16, 2010, 38 soybean allergens have been included in the allergen database (http: / / www.allergenonline .org / / ). Seed structural proteins, storage proteins and defense-related proteins are the main soybean allergenic proteins, among which the storage proteins of allergenic proteins mainly include: Gl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 刘珊珊宋波张淑珍徐鹏飞罗亭亭李明雪
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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