41results about How to "Good research foundation" patented technology

The invention relates to a streaming time series data dimensionality reduction and simplified representation method based on piecewise linear representation. The method comprises the following steps that: S1: presetting data segments and compression parameters; S2: carrying out data scanning on streaming time series data in a slide window way, and entering a streaming data buffer zone; S3: judging whether the fitting error of an initial segmented data segment exceeds an ME_ES (Maximum Error for Entire Segment) or not, carrying out reservation if the fitting error of the initial segmented data segment exceeds the ME_ES, and marking the initial data segment as "inseparable", and if the fitting error of the initial segmented data segment does not exceed the ME_ES, carrying out secondary optimal segmentation; and S4: moving the data segment which is marked as "inseparable" in the streaming data buffer zone out of the streaming data buffer zone, judging whether the streaming time series data to be processed is in the presence or not, if the streaming time series data to be processed is in the presence, returning to the S2, and otherwise, ending. By use of the method, data dimensionality reduction execution efficiency is guaranteed to a high limit, the fitting accuracy of data simplified representation is optimized to a certain range, and accuracy and the execution efficiency of data representation can be improved.

The invention discloses a method for synthesizing ethanol, which comprises the following steps of: 1, preparing oxalic acid diester through coupling reaction of carbon monoxide and nitrite under the catalysis of an alumina-supported palladium catalyst; 2, pre-heating and vaporizing the oxalic acid diester and mixing with hydrogen; carrying out catalytic hydrogenation reaction on the mixed gas on a silicon dioxide-supported copper catalyst, and then cooling and distilling the reaction product to obtain ethanol. The preparation method of the invention realizes over 80% of selectivity and high yield of ethanol, and the gas used in synthesis process can be completely converted through circulation, thereby establishing a good foundation for research on the industry of synthesizing ethanol frombiomass.

The invention discloses a c DNA (Complementary Desoxvribose Nucleic Acid) of a cotton bollworm COPI Beta gene and an application of the c DNA. A COPI Beta si (Small Interfering) RNA of a cotton bollworm is designed and synthesized, so that the expression of the COPI Beta gene is blocked after the COPI Beta si RNA is fed to the cotton bollworm, thus the death rate of the cotton bollworm is improved. Therefore, the biological prevention and control of the cotton bollworm is realized.

The invention discloses a rapid propagation and seedling growing method for Shuhua camellia tissue culture. The rapid propagation and seedling growing method comprises the following steps of: selecting an explant of Shuhua camellia, pretreating and sterilizing; carrying out induced differential culture on an induced culture medium, and carrying out enrichment culture on a multiplication culture medium; then transplanting aseptic seedlings obtained through multiplication directly into a matrix for rooting. The rapid propagation and seedling growing method provided by the invention discloses that the camellia can be subjected to rooting culture without an aseptic condition firstly, and the aseptic seedlings cultured by the multiplication are directly treated by rooting liquid, and then are cut into the gardening matrix for the rooting; the rooting rate is above 90%; the transplanting survival rate is above 86%; the problem of camellia tissue culture seedlings are difficult in the rooting is solved; the production steps are simplified; the cultivation cycle is shortened to be within 5 months; the explant multiplication rate is above 300%; the production efficiency is improved; the market cycle is shortened greatly; the requirement of factory-like rapid propagation and seedling growing can be met.

The invention discloses helicoverpa armigera V-ATPase A gene cDNA and application thereof. Helicoverpa armigera V-ATPase A siRNA is designed and synthesized, after helicoverpa armigera takes V-ATPase A siRNA in, expression of V-ATPase A gene is blocked, growth and metabolism of helicoverpa armigera are inhibited, the mortality is raised, and therefore helicoverpa armigera V-ATPase A gene cDNA is applicable to biological control of helicoverpa armigera.

The invention provides a large-size multifunctional interface dynamic shear tester and a test method, and belongs to the technical field of geotechnical engineering. The tester comprises an oil sourcesystem, a control system and a shearing system; the shearing system is the main body device for carrying out a shearing test, and the shearing system comprises a main frame, a vertical and horizontalactuator, an up-and-down shearing box, a test auxiliary tool, the vertical and horizontal actuator is respectively connected with the up-and-down shearing box, and the test auxiliary tool comprises atooth grabbing plate, a side limiting frame, a soil grabbing sensor and the like; the oil source system is a power device and is used for providing power to two actuators; the control system is the tester control and the sensor signal collection device, and is used for accurately controlling and monitoring the operation state of the tester. By accurately controlling the motion state of the two actuators, various test functions such as different interface types, test materials and different power shearing modes can be realized, and the defects of the current interface dynamic shear test technical means are filled.

The invention belongs to the technical field of gene engineering, and particularly relates to a porcine UCOE regulatory element fragment for enhancing exogenous gene expression. The base sequence of the UCOE regulatory element fragment is shown in a sequence table. With respect to exogenous protein expression, the regulatory element fragment can enhance GFP expression by constructing recombinant plasmid expression vector pCpG-Mini-GFP-UCOE and transfecting HEK293T cells. According to the invention, with a human 1.5 kb UCOE fragment used as a control, a porcine UCOE fragment is cut into fragments with different lengths by an enzyme digestion method and an RT-PCR method; different expression vectors are constructed, and transfected into HEK293T cells; the relative expression level of gene GFP is reported by real-time fluorescence quantification PCR and Western blot technique detection, and the optimal UCOE fragment sequence for enhancing exogenous gene expression is screened, which is the gene sequence claimed in the invention.

The invention belongs to the field of biological gene engineering. A chilo venosatus walker ecdysis regulation transcription factor CsHR3 contains sequences shown by SEQ ID NO.1 and SEQ ID NO.2. The cloning method provided by the invention comprises the following steps of: extracting total RNA (ribonucleic acid) from chilo venosatus walker larvas in prepupal period, and carrying out reverse transcription to synthesize cDNA; designing a degenerate primer according to conserved sequences of different lepidopterous insect ecdysis regulation transcription factors, carrying out PCR (polymerase chain reaction) amplification on the intermediate segment of the chilo venosatus walker CsHR3 gene, designing a specific primer by adopting 3'-RACE (rapid-amplification of cDNA ends) technology, and then obtaining the 1266bp segment of the CsHR3 gene successfully. According to the invention, dsRNA is expressed in RNaseIII defective escherichia coli HT 115 by adopting a bacterium mediate RNAi technology to obtain dsRNA with biological activity, and the chilo venosatus walker ecdysis regulation transcription factor CsHR3 gene is silenced by feeding the chilo venosatus walker larvas, thus chilo venosatus walker can not complete ecdysis or normal growth and development process of the chilo venosatus walker is destroyed and biological prevention and control on the chilo venosatus walker is realized; or the chilo venosatus walker ecdysis regulation transcription factor is converted into a crop, and the crop with insect resistance is obtained.

The invention belongs to the technical field of lung cancer gefitinib drug-resistance model construction, and particularly relates to a method for constructing a non-small-cell lung cancer gefitinib drug-resistance PDX model. The method includes the steps of constructing a non-small-cell lung cancer PDX model, screening a gefitinib-sensitive non-small-cell lung cancer PDX model and induction constructing of the non-small-cell lung cancer gefitinib drug-resistance PDX model. The model can be used as a substitution of the PDX model which is directly established by non-small-cell lung cancer gefitinib drug-resistance tumor issue. The good research basis is provided for studying gefitinib drug-resistance mechanisms, screening target spots and small molecule drugs in the gefitinib drug-resistance process and hence delaying or interfering of the gefitinib drug-resistance process, and good application and popularization value is achieved.

The invention discloses myzuspersicae hunchback gene cDNA and an application thereof; the cDNA of a key gene of hunchback (Mphb) for myzuspersicae embryonic development is transferred to a plant so as to obtain a transgenic plant which expresses Mphb dsRNA and inhibits myzuspersicae reproduction; when a myzuspersicae takes the transgenic tobacco, the expression of Mphb is inhibited; the embryonic development of the myzuspersicae is blocked; the daily mean myzuspersicae producing number is decreased; the reproductive capacity is reduced; and thus the myzuspersicae hunchback gene cDNA can be used for myzuspersicae biological control.

The invention discloses a method for synthesizing ethanol, which comprises the following steps of: 1, preparing oxalic acid diester through coupling reaction of carbon monoxide and nitrite under the catalysis of an alumina-supported palladium catalyst; 2, pre-heating and vaporizing the oxalic acid diester and mixing with hydrogen; carrying out catalytic hydrogenation reaction on the mixed gas on a silicon dioxide-supported copper catalyst, and then cooling and distilling the reaction product to obtain ethanol. The preparation method of the invention realizes over 80% of selectivity and high yield of ethanol, and the gas used in synthesis process can be completely converted through circulation, thereby establishing a good foundation for research on the industry of synthesizing ethanol from biomass.

The invention discloses an OsPEAMT2 gene for increasing the heading stage maturing rate of rice under high-temperature stress as well as a protein and application of the OsPEAMT2 gene. The nucleotidesequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence of the protein is shown as SEQ ID NO. 2. The invention provides a new rice ear stage high temperature related gene, and provides a new gene resource for improvement of rice yield traits and breeding utilization of a rice thermo-sensitive sterile line.

The invention provides an RGD-recombinatn staphylokinase-human alpha microglobulin fusion protein, and a preparation method and an application thereof. The amino acid sequence of the RGD-recombinatn staphylokinase-human alpha microglobulin fusion protein is represented by SEQ ID NO.12. The preparation method of the fusion protein comprises the following steps: obtaining a target gene fusion fragment through adopting an overlap extension PCR technology; inserting the target gene fusion fragment into an expression vector to construct a recombinant expression plasmid; transforming the constructed recombinant expression plasmid into Escherichia coli, and allowing the recombinant expression plasmid to highly express in the Escherichia coli; and carrying out fusion protein separation and purification. Compared with present products of same kind, the fusion protein has the advantages of efficient thrombolysis and anti-coagulating activity and low immunogenicity. The fusion protein also can overcome the disadvantages of activity decrease and insoluble expression of some present RGD-recombinant staphylokinases.

The invention discloses an OsALIS4 gene for reducing the rice setting rate, a protein obtained by coding the OsALIS4 gene and application of the OsALIS4 gene. The nucleotide sequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO. 2. The invention provides a new idea for clarifying the setting rate, namely a molecular mechanism related to complex agronomic traits, and provides a potential new gene resource for rice breeding.

The invention discloses an extraction method of laminaria japonica aresch lectin crude extract. The extraction method is characterized by comprising the steps of washing laminaria japonica aresch with filtering seawater, absorbing the moisture on the surface of laminaria japonica aresch, then freezing laminaria japonica aresch to minus 20 DEG C, then drying laminaria japonica aresch at 40-60 DEG C, grinding dried laminaria japonica aresch into powder, taking a TBS (t-butyldimethylsilane) buffer solution with a pH value of 7.5-8.0, soaking the laminaria japonica aresch powder in the TBS buffer solution in a liquid-material ratio of (15:1)-(25:1) for 15-20 hours, centrifuging the solution with a centrifuge for 30 minutes under the conditions of 5000r / min and 4 DEG C, and taking the supernatant, thus obtaining the laminaria japonica aresch lectin crude extract. The laminaria japonica aresch lectin crude extract with relatively high agglutination activity can be obtained by utilizing the method.

The invention discloses a specific CAPS molecular marker snp545 for identifying hypoallergenic specific soybean variety, as well as a method and an application thereof, which relate to the field of molecular markers, and provides the specific CAPS molecular marker for identifying the hypoallergenic specific soybean variety, the method and the application thereof, and the nucleotide sequence of theCAPS molecular marker is shown as SEQ ID NO: 1. The snp545 marker disclosed by the invention is used for identifying the hypoallergenic specific soybean variety, and the hypoallergenic specific soybean variety is an alpha-subunit deletion variety, so that a good research basis is provided for excavation and identification analysis of alpha-subunit deletion candidate genes. Through multiple tests,the result is stable and reliable. The method is applied to the field of low-sensitivity special soybean breeding.

The invention discloses myzuspersicae hunchback gene cDNA and an application thereof; the cDNA of a key gene of hunchback (Mphb) for myzuspersicae embryonic development is transferred to a plant so as to obtain a transgenic plant which expresses Mphb dsRNA and inhibits myzuspersicae reproduction; when a myzuspersicae takes the transgenic tobacco, the expression of Mphb is inhibited; the embryonic development of the myzuspersicae is blocked; the daily mean myzuspersicae producing number is decreased; the reproductive capacity is reduced; and thus the myzuspersicae hunchback gene cDNA can be used for myzuspersicae biological control.

The invention discloses application of recombinant human insulin-like growth factor 1 (rhIGF1) in preparation of medicines for treating fragile X syndrome. The research of the invention fully proves that the level of insulin-like growth factor 1 (IGF1) in serum and brain hippocampus of a postnatal mouse with the fragile X syndrome is significantly lower than that of normal mice of the same age, and the intraperitoneal injection of the rhIGF1 can improve the level of the IGF1 of the mouse with the fragile X syndrome and further enables the level of the IGF1 to be similar to that of normal miceof the same age. The rhIGF1 has a good therapeutic effect on the core symptoms, such as hippocampal neuronal dysplasia, learning memory deficit, impaired social interaction, anxiety and giant testicular disease, of the fragile X syndrome; the effect is related to regulation on excessive activation of an IGFR1-AKT-S6K signaling pathway. The rhIGF1 is successfully used in the treatment of Rett syndrome and insulin resistance in clinical practice, has good safety, and has the advantages of being sufficient in research basis and short in possible research period compared with other therapeutic drugs under development.

The invention belongs to the technical field of textile articles, and particularly relates to a preparation method of a carrier capable of regularly prompting textile replacement, and the carrier. The preparation method comprises the steps of adopting a mixed solution of lactone violet extracted from perilla frutescens and subjected to a chelation reaction, a melamino-formaldehyde resin prepolymer and polyvinyl alcohol as a raw material; carrying out in-situ polymerization reaction to obtain a lactone violet water-in-oil microcapsule; and implanting the lactone violet water-in-oil microcapsule on the carrier, and preparing the carrier capable of regularly prompting textile replacement, so that people can be prompted to regularly replace a textile, and the problems of bacterial infection and the like caused by non-replacement for a long time are solved. According to the preparation method of the carrier capable of regularly prompting textile replacement, and the carrier provided by the invention, a new development direction and a new development path are given to the textile field, and good research foundation and reference value are laid for development in the aspects of intelligent visual textiles, healthy and safe use of the textiles and the like in the future.

The invention discloses a bending-resistant carbon nanotube / graphene composite film, a preparation method and application thereof. The carbon nanotube / graphene composite film has a porous network structure, and the composite film includes a porous structure formed by expansion of graphene sheets and carbon nanotubes, wherein at least part of the carbon nanotubes are distributed in the graphene sheets A three-dimensional network structure is formed between them. The preparation method includes: sequentially carbonizing and graphitizing the carbon nanotube / graphene oxide composite film in a protective atmosphere to obtain a bending-resistant carbon nanotube mainly composed of carbon nanotubes and reduced graphene oxide. tube / graphene composite film. In the present invention, the carbon nanotube / graphene oxide composite film is carbonized and graphitized, and the carbon nanotube is used to form a three-dimensional network structure between the graphene sheets, so as to obtain a composite film with high bending resistance and excellent bending resistance. A carbon nanotube / graphene composite film with good electromagnetic shielding properties.

The invention discloses application of dahurian patrinia herb to preparation of a medicine for resisting methicillin-resistant staphylococcus epidermidis, the medicine for resisting methicillin-resistant staphylococcus epidermidis is prepared by taking dahurian patrinia herb or a dahurian patrinia herb extract as a raw material, the minimum inhibitory concentration of the dahurian patrinia herb extract to the methicillin-resistant staphylococcus epidermidis is 2.5 mg / mL, and the minimum bactericidal concentration of the dahurian patrinia herb extract to the methicillin-resistant staphylococcus epidermidis is 20 mg / mL. According to the application disclosed by the invention, the new application of the herba patriniae in preparing the medicine for treating and resisting the methicillin-resistant staphylococcus epidermidis is found for the first time, a new medication way is brought for clinically treating infection caused by the methicillin-resistant staphylococcus epidermidis, and the application has important clinical significance for preventing and treating infection caused by the methicillin-resistant staphylococcus epidermidis.

The invention belongs to the field of biological gene engineering. A chilo venosatus walker ecdysis regulation transcription factor CsHR3 contains sequences shown by SEQ ID NO.1 and SEQ ID NO.2. The cloning method provided by the invention comprises the following steps of: extracting total RNA (ribonucleic acid) from chilo venosatus walker larvas in prepupal period, and carrying out reverse transcription to synthesize cDNA; designing a degenerate primer according to conserved sequences of different lepidopterous insect ecdysis regulation transcription factors, carrying out PCR (polymerase chain reaction) amplification on the intermediate segment of the chilo venosatus walker CsHR3 gene, designing a specific primer by adopting 3'-RACE (rapid-amplification of cDNA ends) technology, and then obtaining the 1266bp segment of the CsHR3 gene successfully. According to the invention, dsRNA is expressed in RNaseIII defective escherichia coli HT 115 by adopting a bacterium mediate RNAi technology to obtain dsRNA with biological activity, and the chilo venosatus walker ecdysis regulation transcription factor CsHR3 gene is silenced by feeding the chilo venosatus walker larvas, thus chilo venosatus walker can not complete ecdysis or normal growth and development process of the chilo venosatus walker is destroyed and biological prevention and control on the chilo venosatus walker is realized; or the chilo venosatus walker ecdysis regulation transcription factor is converted into a crop, and the crop with insect resistance is obtained.

A specific CAPS molecular marker snp545 and its application for identifying low-allergenic special soybean varieties, which relate to the field of molecular markers. The present invention provides a specific low-allergenic soybean variety-specific CAPS molecular markers and methods thereof Application, the nucleotide sequence of the CAPS molecular marker is shown in SEQ ID NO:1. The snp545 marker of the present invention is used to identify low-allergenic special-purpose soybean varieties, and the low-allergenic special-purpose soybean varieties are α-subunit-deleted varieties, which provide a good method for mining and identification analysis of α-subunit-deleted candidate genes research basis. After many tests, the results are stable and reliable. The invention is applied to the field of low-allergenic special-purpose soybean breeding.

The invention discloses a human micro-ecosystem chip, and belongs to the field of medical research and human body related monitoring application. A sandwich structure of "PDMS A plate-porous membrane-PDMS B plate" is used for realizing a lower layer PDMS A plate to construct intestine-liver-heart-brain-female genital duct system to integrate an "organ chip" to simulate real human blood circulation; human microorganisms are cultured in an upper layer PDMS B plate; and a porous membrane filters human microbial metabolites from the medium containing human microorganisms, and the PDMS B plate enters the PDMS A plate. In the PDMS A plate, a micro valve is provided for micro flow between cell culture chambers, and the medium flow is adjusted by controlling the micro valve. The human micro-ecosystem chip can be used for accurately, concisely and effectively research, evaluate and control the interaction between the human microbial metabolites and multiple "organs" of an individual; and by culturing corresponding human cells in the culture chambers and controlling the flow of the culture medium, the bionics of cerebrospinal fluid circulation and direct nerve connection existing in a real human body can realized, and a research foundation is laid for medical research and human body related monitoring application.

The present invention discloses an OsALIS4 gene for reducing seed setting rate of rice and its encoded protein and application. The nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown in SEQ ID NO. 2. The invention provides a new idea for elucidating the molecular mechanism related to complex agronomic traits such as seed setting rate, and provides potential new gene resources for rice breeding.

The invention discloses a c DNA (Complementary Desoxvribose Nucleic Acid) of a cotton bollworm COPI Beta gene and an application of the c DNA. A COPI Beta si (Small Interfering) RNA of a cotton bollworm is designed and synthesized, so that the expression of the COPI Beta gene is blocked after the COPI Beta si RNA is fed to the cotton bollworm, thus the death rate of the cotton bollworm is improved. Therefore, the biological prevention and control of the cotton bollworm is realized.

The invention discloses an in-situ electrochemical immunosensor for simultaneously detecting four markers of breast cancer. Molecular typing is performed on the breast cancer by detecting the four markers HER2, ER, PR and Ki67 in serum, and different metal ions are used as detection signals. And a magnetic bead-protein-silicon dioxide sandwich structure is formed for simultaneous detection of four markers. The sensor takes serum as a detection sample, breast cancer tissues do not need to be extracted, the operation is simple, the anti-interference capability is strong, a detection result is typed through an electrochemical signal of an electrochemical workstation, the repeatability is good, the accuracy is high, the detection limit is low, the whole reaction process can be shortened to 140 minutes, and the detection efficiency is greatly improved. The method has potential application value in diagnosis of different types of cancers.

The invention discloses a method for inducing hermaphrodite flowers of Luo Han Guo, which comprises the following steps: selecting budding branches and tendrils of Luo Han Guo, wetting the end of the budding branches and tendrils with a solution containing an ethylene synthesis inhibitor or an ethylene signal transduction inhibitor for 10 seconds to 8 hours, The concentration of the ethylene synthesis inhibitor in the solution is 1-300 mg / L, the concentration of the ethylene signal transduction inhibitor in the solution is 25-500 mg / L, and the ethylene synthesis inhibitor is (S)-trans-2-amino-4 ‑(2‑aminoethoxy)‑3‑butenoic acid, 2‑aminoisobutyric acid, 1‑methylcyclopropene, the ethylene signal transduction inhibitor is a compound containing silver ions, such as silver nitrate, sulfur Any of the silver sulfosulfates. The invention provides a good research basis for the research on the sex regulation mechanism and gene mining of Luo Han Guo.

The invention discloses a MAP3K‑19 gene for improving the high temperature tolerance of rice at the panicle stage, a protein encoded by it, and an application thereof. The nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown in SEQ ID NO.2. The invention provides a new rice panicle-stage high-temperature resistance-related gene, and provides new genetic resources for rice high-temperature-resistant breeding and rice temperature-sensitive male sterile line breeding.

The invention belongs to the technical field of gene engineering, and particularly relates to a porcine UCOE regulatory element fragment for enhancing exogenous gene expression. The base sequence of the UCOE regulatory element fragment is shown in a sequence table. With respect to exogenous protein expression, the regulatory element fragment can enhance GFP expression by constructing recombinant plasmid expression vector pCpG-Mini-GFP-UCOE and transfecting HEK293T cells. According to the invention, with a human 1.5 kb UCOE fragment used as a control, a porcine UCOE fragment is cut into fragments with different lengths by an enzyme digestion method and an RT-PCR method; different expression vectors are constructed, and transfected into HEK293T cells; the relative expression level of gene GFP is reported by real-time fluorescence quantification PCR and Western blot technique detection, and the optimal UCOE fragment sequence for enhancing exogenous gene expression is screened, which is the gene sequence claimed in the invention.