41results about How to "Good research foundation" patented technology

The invention discloses a method for synthesizing ethanol, which comprises the following steps of: 1, preparing oxalic acid diester through coupling reaction of carbon monoxide and nitrite under the catalysis of an alumina-supported palladium catalyst; 2, pre-heating and vaporizing the oxalic acid diester and mixing with hydrogen; carrying out catalytic hydrogenation reaction on the mixed gas on a silicon dioxide-supported copper catalyst, and then cooling and distilling the reaction product to obtain ethanol. The preparation method of the invention realizes over 80% of selectivity and high yield of ethanol, and the gas used in synthesis process can be completely converted through circulation, thereby establishing a good foundation for research on the industry of synthesizing ethanol from biomass.

The invention discloses myzuspersicae hunchback gene cDNA and an application thereof; the cDNA of a key gene of hunchback (Mphb) for myzuspersicae embryonic development is transferred to a plant so as to obtain a transgenic plant which expresses Mphb dsRNA and inhibits myzuspersicae reproduction; when a myzuspersicae takes the transgenic tobacco, the expression of Mphb is inhibited; the embryonic development of the myzuspersicae is blocked; the daily mean myzuspersicae producing number is decreased; the reproductive capacity is reduced; and thus the myzuspersicae hunchback gene cDNA can be used for myzuspersicae biological control.

The invention discloses a bending-resistant carbon nanotube / graphene composite film, a preparation method and application thereof. The carbon nanotube / graphene composite film has a porous network structure, and the composite film includes a porous structure formed by expansion of graphene sheets and carbon nanotubes, wherein at least part of the carbon nanotubes are distributed in the graphene sheets A three-dimensional network structure is formed between them. The preparation method includes: sequentially carbonizing and graphitizing the carbon nanotube / graphene oxide composite film in a protective atmosphere to obtain a bending-resistant carbon nanotube mainly composed of carbon nanotubes and reduced graphene oxide. tube / graphene composite film. In the present invention, the carbon nanotube / graphene oxide composite film is carbonized and graphitized, and the carbon nanotube is used to form a three-dimensional network structure between the graphene sheets, so as to obtain a composite film with high bending resistance and excellent bending resistance. A carbon nanotube / graphene composite film with good electromagnetic shielding properties.

The invention belongs to the field of biological gene engineering. A chilo venosatus walker ecdysis regulation transcription factor CsHR3 contains sequences shown by SEQ ID NO.1 and SEQ ID NO.2. The cloning method provided by the invention comprises the following steps of: extracting total RNA (ribonucleic acid) from chilo venosatus walker larvas in prepupal period, and carrying out reverse transcription to synthesize cDNA; designing a degenerate primer according to conserved sequences of different lepidopterous insect ecdysis regulation transcription factors, carrying out PCR (polymerase chain reaction) amplification on the intermediate segment of the chilo venosatus walker CsHR3 gene, designing a specific primer by adopting 3'-RACE (rapid-amplification of cDNA ends) technology, and then obtaining the 1266bp segment of the CsHR3 gene successfully. According to the invention, dsRNA is expressed in RNaseIII defective escherichia coli HT 115 by adopting a bacterium mediate RNAi technology to obtain dsRNA with biological activity, and the chilo venosatus walker ecdysis regulation transcription factor CsHR3 gene is silenced by feeding the chilo venosatus walker larvas, thus chilo venosatus walker can not complete ecdysis or normal growth and development process of the chilo venosatus walker is destroyed and biological prevention and control on the chilo venosatus walker is realized; or the chilo venosatus walker ecdysis regulation transcription factor is converted into a crop, and the crop with insect resistance is obtained.

A specific CAPS molecular marker snp545 and its application for identifying low-allergenic special soybean varieties, which relate to the field of molecular markers. The present invention provides a specific low-allergenic soybean variety-specific CAPS molecular markers and methods thereof Application, the nucleotide sequence of the CAPS molecular marker is shown in SEQ ID NO:1. The snp545 marker of the present invention is used to identify low-allergenic special-purpose soybean varieties, and the low-allergenic special-purpose soybean varieties are α-subunit-deleted varieties, which provide a good method for mining and identification analysis of α-subunit-deleted candidate genes research basis. After many tests, the results are stable and reliable. The invention is applied to the field of low-allergenic special-purpose soybean breeding.

The present invention discloses an OsALIS4 gene for reducing seed setting rate of rice and its encoded protein and application. The nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown in SEQ ID NO. 2. The invention provides a new idea for elucidating the molecular mechanism related to complex agronomic traits such as seed setting rate, and provides potential new gene resources for rice breeding.

The invention discloses a c DNA (Complementary Desoxvribose Nucleic Acid) of a cotton bollworm COPI Beta gene and an application of the c DNA. A COPI Beta si (Small Interfering) RNA of a cotton bollworm is designed and synthesized, so that the expression of the COPI Beta gene is blocked after the COPI Beta si RNA is fed to the cotton bollworm, thus the death rate of the cotton bollworm is improved. Therefore, the biological prevention and control of the cotton bollworm is realized.

The invention discloses a method for inducing hermaphrodite flowers of Luo Han Guo, which comprises the following steps: selecting budding branches and tendrils of Luo Han Guo, wetting the end of the budding branches and tendrils with a solution containing an ethylene synthesis inhibitor or an ethylene signal transduction inhibitor for 10 seconds to 8 hours, The concentration of the ethylene synthesis inhibitor in the solution is 1-300 mg / L, the concentration of the ethylene signal transduction inhibitor in the solution is 25-500 mg / L, and the ethylene synthesis inhibitor is (S)-trans-2-amino-4 ‑(2‑aminoethoxy)‑3‑butenoic acid, 2‑aminoisobutyric acid, 1‑methylcyclopropene, the ethylene signal transduction inhibitor is a compound containing silver ions, such as silver nitrate, sulfur Any of the silver sulfosulfates. The invention provides a good research basis for the research on the sex regulation mechanism and gene mining of Luo Han Guo.