OsALIS4 gene for reducing rice setting rate, protein obtained by coding OsALIS4 gene and application of OsALIS4 gene

A technology of seed setting rate and genes, applied in the fields of biotechnology and breeding, which can solve problems such as functional redundancy

Active Publication Date: 2020-12-04
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, studies in different species have shown that the conservation of this type of gene determines the high degree of functional redundancy between them, and there are few reports about this type of gene alone controlling a certain type of biological function

Method used

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  • OsALIS4 gene for reducing rice setting rate, protein obtained by coding OsALIS4 gene and application of OsALIS4 gene
  • OsALIS4 gene for reducing rice setting rate, protein obtained by coding OsALIS4 gene and application of OsALIS4 gene
  • OsALIS4 gene for reducing rice setting rate, protein obtained by coding OsALIS4 gene and application of OsALIS4 gene

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Embodiment

[0035] A preferred embodiment of the present invention provides the application of the OsALIS4 gene in reducing the seed setting rate of rice, specifically including:

[0036] 1. Obtaining homologous gene sequences and making phylogenetic trees

[0037] The protein sequences of yeast, Arabidopsis, and human are all from NCBI, rice is from the Rice GenomeAnnotation Project, and nematodes are from Worm Base. Among them, the codes for obtaining rice protein sequences are as follows: OsALIS1 (LOC_Os09g38768), OsALIS2 (LOC_Os02g07750), OsALIS3 (LOC_Os06g45430), OsALIS4 (LOC_Os03g02830), OsALIS5 (LOC_Os05g45370), OsALIS6 (LOC_Os703g57O) Clustal Omega was used to align and MEGA5.1 was used to make a phylogenetic tree. The sequence analysis results are as figure 1 As shown, there are 6 CDS50 genes in rice. According to the nomenclature of Arabidopsis thaliana, they were named OsALIS1-6, and OsALIS1-5 were all on the same evolutionary branch, and then OsALIS4 was studied.

[0038] 2...

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Abstract

The invention discloses an OsALIS4 gene for reducing the rice setting rate, a protein obtained by coding the OsALIS4 gene and application of the OsALIS4 gene. The nucleotide sequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO. 2. The invention provides a new idea for clarifying the setting rate, namely a molecular mechanism related to complex agronomic traits, and provides a potential new gene resource for rice breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology and breeding, and in particular relates to an OsALIS4 gene for reducing the seed setting rate of rice and its encoded protein and application. Background technique [0002] Rice is an important food crop and is the staple food for half of the world's population. The yield of rice is mainly affected by several factors such as the number of grains per panicle, effective panicle per plant, thousand-grain weight and seed setting rate. In the past ten years, the research on rice panicle development and grain weight has made remarkable progress. However, as an important yield component, the research on seed setting rate still needs to be promoted urgently. In view of this, it is of great practical significance for rice genetics and breeding to discover and clone more genes related to seed setting rate and analyze their genetic mechanism. [0003] Existing studies have shown that seed setting rate is a ver...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/08A01H6/46
CPCC07K14/415C12N15/8218C12N15/8261
Inventor 樊世军胥磊梁潆俪高鹏何凯袁华涂斌马炳田王玉平钦鹏李仕贵
Owner SICHUAN AGRI UNIV
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