Extraction method of laminaria japonica aresch lectin crude extract

An extraction method, a rough extraction technology, which is applied in the field of protein or glycoprotein extraction, can solve the problems that have not been seen in the extraction process of kelp lectin, and achieve the effect of ensuring the agglutination activity

Inactive Publication Date: 2014-03-26
DALIAN OCEAN UNIV +1
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  • Summary
  • Abstract
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  • Claims
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Problems solved by technology

At present, there are more and more studies on the extraction process of lectins at home and abroad. People have studied the extraction process of crude mistletoe lectin, the extraction process and activity determination of leek lectin, the optimization of the extraction process of Chaga lectin, and the extraction of mulberry leaf lectin. Research on process optimization, etc., but there is no report on the extraction process of kelp lectin

Method used

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Embodiment Construction

[0008] Specific embodiments of the present invention will be described below.

[0009] A method for extracting a crude extract of kelp agglutinin is carried out according to the following steps: first take fresh kelp, wash it with filtered sea water, and use gauze filter paper to absorb the surface moisture of the kelp, and freeze the kelp. When the temperature of the kelp drops After the temperature reaches -20℃, it is dried at a temperature of 40-60℃, where 50℃ is the best drying temperature; the dried kelp is ground into powder, and the TBS (Tris- HCl) buffer, the pH of which is 7.7 is the best pH; put the kelp powder into TBS buffer for soaking, the mixed liquid-to-material ratio is 15-25:1, the soaking time is 15-20h, among which the best solution The material ratio is 20:1, and the best soaking time is 20h; in order to obtain better results, it is best to keep stirring during the soaking process, and then put the mixed solution on the centrifuge at 5000r / min and 4℃ Centrif...

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Abstract

The invention discloses an extraction method of laminaria japonica aresch lectin crude extract. The extraction method is characterized by comprising the steps of washing laminaria japonica aresch with filtering seawater, absorbing the moisture on the surface of laminaria japonica aresch, then freezing laminaria japonica aresch to minus 20 DEG C, then drying laminaria japonica aresch at 40-60 DEG C, grinding dried laminaria japonica aresch into powder, taking a TBS (t-butyldimethylsilane) buffer solution with a pH value of 7.5-8.0, soaking the laminaria japonica aresch powder in the TBS buffer solution in a liquid-material ratio of (15:1)-(25:1) for 15-20 hours, centrifuging the solution with a centrifuge for 30 minutes under the conditions of 5000r/min and 4 DEG C, and taking the supernatant, thus obtaining the laminaria japonica aresch lectin crude extract. The laminaria japonica aresch lectin crude extract with relatively high agglutination activity can be obtained by utilizing the method.

Description

Technical field [0001] The invention relates to a method for extracting protein or glycoprotein, in particular to a method for extracting kelp agglutinin. Background technique [0002] Lectin is a kind of sugar-specific protein or glycoprotein that can promote cell aggregation. It has a variety of biological activities such as agglutinating cells, inhibiting tumor cell proliferation, and inhibiting platelet aggregation. kelp( Laminaria japonica Aresch) belongs to the Phaeophyta Kelpaceae and Laminaria genus, also known as kelp; it has the characteristics of wide distribution, large algae, and fast growth. It is a seaweed with large individuals, soft texture, and high nutritional value and economic value. . Regarding the seaweed in the Phaeophyta, the separation and some properties of the wakame and kelp agglutinin have been studied. In addition, people have isolated and purified other algae, such as Thousand Island Carrageenan, Ulva vulgaris, Enteromorpha intestines, Gracilari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/405C07K1/14
CPCC07K14/405
Inventor 李丹彤李悦尹建鹏李帅李晓丽
Owner DALIAN OCEAN UNIV
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