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C DNA (Complementary Desoxvribose Nucleic Acid) of cotton bollworm COPI Beta gene and application of c DNA

A technology of cotton bollworm and β gene, which is applied in the field of insect molecular biology and physiology, and can solve the problem of silencing pest target genes with few siRNAs

Inactive Publication Date: 2014-06-11
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on silencing pest target genes by siRNA

Method used

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  • C DNA (Complementary Desoxvribose Nucleic Acid) of cotton bollworm COPI Beta gene and application of c DNA
  • C DNA (Complementary Desoxvribose Nucleic Acid) of cotton bollworm COPI Beta gene and application of c DNA
  • C DNA (Complementary Desoxvribose Nucleic Acid) of cotton bollworm COPI Beta gene and application of c DNA

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Embodiment Construction

[0026]The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.

[0027] The present invention clones the cDNA of the coat protein Iβ subunit (COPIβ) from the cotton bollworm, which has the sequence shown in SEQ ID NO.1:

[0028] (1) Information of SEQ ID NO.1

[0029] (a) Sequence features:

[0030] Sequence length: 1397 base pairs

[0031] Type: nucleic acid

[0032] Chain type: double chain

[0033] Topology: Linear

[0034] (b) Molecular type: cDNA

[0035] (c) Assumption: No

[0036] (d) Antisense: no

[0037] (e) Original source: Cotton bollworm (Helicoverpa armigera)

[0038] (f) Sequence description: SEQ ID NO.1

[0039] (2) Information of SEQ ID NO.2

[0040] (a) Sequential features

[0041] Sequence length: 465 amino acids

[0042] Type: amino acid

[0043] Chain type: single chain

[0044] Topology: Linear

[0045] (b...

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Abstract

The invention discloses a c DNA (Complementary Desoxvribose Nucleic Acid) of a cotton bollworm COPI Beta gene and an application of the c DNA. A COPI Beta si (Small Interfering) RNA of a cotton bollworm is designed and synthesized, so that the expression of the COPI Beta gene is blocked after the COPI Beta si RNA is fed to the cotton bollworm, thus the death rate of the cotton bollworm is improved. Therefore, the biological prevention and control of the cotton bollworm is realized.

Description

technical field [0001] The invention belongs to the field of insect molecular biology and physiology, in particular to the cloning of the cDNA of cotton bollworm coat protein I (coatomerprotein I) β subunit (COPI β), the design, synthesis and identification of small RNA (siRNA) of the cotton bollworm COPI β gene Feeding of cotton bollworm larvae. Background technique [0002] Cotton bollworm (Helicoverpa armigera), a species of Noctuidae insects, is an important borer pest of cotton buds and bolls. It mainly eats buds, flowers, and bolls, and also feeds on young leaves. Cotton bollworm is a pest with worldwide distribution. It is distributed and damaged in every cotton area in China, and the northern cotton area suffers more seriously than the southern cotton area. Cotton bollworm is a polyphagous pest. In addition to damaging cotton, it can also damage crops such as wheat, corn, sorghum, soybean, pea, alfalfa, sesame, tomato, pepper, and sunflower. The host plants are more...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/435C12N15/10C12N15/113A01N57/16A01P7/04
Inventor 毛建军曾凡荣
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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