Human micro-ecological system chip and using method thereof

Abstract

The invention discloses a human micro-ecosystem chip, and belongs to the field of medical research and human body related monitoring application. A sandwich structure of "PDMS A plate-porous membrane-PDMS B plate" is used for realizing a lower layer PDMS A plate to construct intestine-liver-heart-brain-female genital duct system to integrate an "organ chip" to simulate real human blood circulation; human microorganisms are cultured in an upper layer PDMS B plate; and a porous membrane filters human microbial metabolites from the medium containing human microorganisms, and the PDMS B plate enters the PDMS A plate. In the PDMS A plate, a micro valve is provided for micro flow between cell culture chambers, and the medium flow is adjusted by controlling the micro valve. The human micro-ecosystem chip can be used for accurately, concisely and effectively research, evaluate and control the interaction between the human microbial metabolites and multiple "organs" of an individual; and by culturing corresponding human cells in the culture chambers and controlling the flow of the culture medium, the bionics of cerebrospinal fluid circulation and direct nerve connection existing in a real human body can realized, and a research foundation is laid for medical research and human body related monitoring application.

Description

technical field [0001] The invention belongs to the field of medical research and human body-related monitoring applications, and relates to a human micro-ecological system chip integrated with multiple "organ" systems and its use method, which can be used for accurate, streamlined, and efficient research, evaluation, and control of human microbial metabolites and the Interactions between multiple "organs" of an individual. Background technique [0002] Humans live in a huge and complicated ecosystem network woven by a wide variety of microorganisms. In recent years, with the introduction of concepts such as the "gut-brain" axis, researchers have paid more and more attention to the role of gut microbes in human metabolism. Intestinal microorganisms are mainly located in the human gastrointestinal tract, and their secreted metabolites are absorbed by human intestinal epithelial cells into the hepatic portal vein, and then enter the human blood circulation, affecting the meta...

AI Technical Summary

Problems solved by technology

A single "organ chip" is too simple to meet the requirements of the study of the human physiological cycle, and multi-"organ" system integrated chips for the real human physiological cycle are an excellent research solution and have important clinical application value, but in In practice it is still in its infancy

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