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Myzuspersicae hunchback gene cDNA and application thereof

A technology of tobacco aphids and genes, which is applied in the direction of DNA preparation, recombinant DNA technology, DNA / RNA fragments, etc., can solve the problems of research reports on control of tobacco aphids, increase the yield and economic value of tobacco leaves, reduce losses, and have a good research foundation Effect

Inactive Publication Date: 2014-12-10
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But now the aphid hunchback ( Mphb ) has not been cloned, let alone started with Mphb Research report on controlling Aphis persicae as a target

Method used

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  • Myzuspersicae hunchback gene cDNA and application thereof
  • Myzuspersicae hunchback gene cDNA and application thereof
  • Myzuspersicae hunchback gene cDNA and application thereof

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Embodiment Construction

[0043] The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.

[0044] The present invention clones the key gene of embryonic development from Aphid persicae hunchback ( Mphb ), which has the sequence shown in SEQ ID NO.1:

[0045] (1) Information on SEQ ID NO.1

[0046] (a) Sequential features:

[0047] Sequence length: 1325 base pairs

[0048] Type: nucleic acid

[0049] Chain type: double chain

[0050] Topology: Linear

[0051] (b) Molecule type: cDNA

[0052] (c) Assumption: No

[0053] (d) Antisense: No

[0054] (e) Original source: Aphids ( Myzus persicae )

[0055] (f) Sequence description: SEQ ID NO.1

[0056] (2) Information on SEQ ID NO.2

[0057] (a) Sequential features

[0058] Sequence length: 441 amino acids

[0059] Type: amino acid

[0060] Chain type: single chain

[0061] Topology: Linear

[0062] (b) ...

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Abstract

The invention discloses myzuspersicae hunchback gene cDNA and an application thereof; the cDNA of a key gene of hunchback (Mphb) for myzuspersicae embryonic development is transferred to a plant so as to obtain a transgenic plant which expresses Mphb dsRNA and inhibits myzuspersicae reproduction; when a myzuspersicae takes the transgenic tobacco, the expression of Mphb is inhibited; the embryonic development of the myzuspersicae is blocked; the daily mean myzuspersicae producing number is decreased; the reproductive capacity is reduced; and thus the myzuspersicae hunchback gene cDNA can be used for myzuspersicae biological control.

Description

technical field [0001] The invention belongs to the fields of insect molecular biology and plant genetic engineering, and specifically relates to a key gene for embryonic development of aphids hunchback ( Mphb ) cDNA cloning, expression Mphb Obtaining dsRNA transgenic tobacco. Background technique [0002] Piercing-sucking mouthparts pests are an important class of agricultural pests, mainly including Homoptera, Hemiptera, and some Diptera insects, such as rice planthoppers, rice leafhoppers, cotton Lygus and various aphids. Piercing-sucking mouthparts pests often live in groups on twigs, leaves, buds, flower buds, and fruits, absorbing plant juices and plundering their nutrients, causing branches, leaves and flowers to curl, and even the whole plant to wither or die. For example, rice planthoppers generally damage 20% to 30% of the damage, and seriously damage 30% to 50% of the damage, or even no harvest. In recent years, the cotton bug has risen to be the main pest ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/12C12N15/10C12N15/113C12N15/82C07K14/435A01H5/00
Inventor 毛建军曾凡荣
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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