Method for constructing mice with specific beta-catenin knockout mature fat cells

A technology of mature adipocytes and construction method, which is applied in the field of construction of mature adipocyte-specific β-catenin knockout mice, can solve the problem of unclear β-catenin, the core molecule of Wnt signaling pathway, etc.

Inactive Publication Date: 2020-04-10
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, how the in vivo level of β-catenin, a core molecule of the Wnt signaling pathway, regulates fat function remains unclear.

Method used

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  • Method for constructing mice with specific beta-catenin knockout mature fat cells
  • Method for constructing mice with specific beta-catenin knockout mature fat cells
  • Method for constructing mice with specific beta-catenin knockout mature fat cells

Examples

Experimental program
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Effect test

Embodiment 1

[0053] 1. Adiponectin-Cre; β-catenin flox / flox Knockout mouse breeding:

[0054] β-catenin in the C57BL / 6 background flox / flox Mice mated with Adiponectin-Cre mice to obtain Adiponectin-Cre; β-catenin flox / wt After mice, with β-catenin flox / flox Mice were mated and control mouse β-cateninf was obtained in the offspring lox / flox and knockout mice Adiponectin-Cre; β-catenin flox / flox , that is, mature adipocyte-specific β-catenin knockout mice, referred to as ABKO knockout mice. Adiponectin-Cre; β-catenin was used in subsequent propagation flox / flox Mate with β-cateninflox / flox mice.

[0055] The mice were kept in the SPF-level barrier environment of the Animal Science Department of Shanghai Jiaotong University School of Medicine and Shanghai Southern Model Biotechnology Co., Ltd. All animals were raised in standard mouse cages under the following conditions: ambient temperature 20-22 °C; free access to food And drinking water, standard commercial mouse feed (4.5% fat, 4%...

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Abstract

The invention relates to a method for constructing mice with specific beta-catenin knockout mature fat cells. According to the method, the condition that beta-catenin can inhibit transcriptional expression of Saa3 is verified, how classical Wnt signal channels affect functions of the mature fat cells and then affect occurrence of obesity is disclosed, or a new way of think is provided for obesitytreatment.

Description

technical field [0001] The invention belongs to the field of obesity treatment, and in particular relates to a method for constructing mature adipocyte-specific β-catenin knockout mice. Background technique [0002] Numerous studies have confirmed that the Wnt signaling pathway is a key pathway that regulates the development of adipose tissue. OrmondA.MacDougald published a study in 2000 and found that overexpression of WNT1 or activated β-catenin in 3T3-L1 cells can inhibit adipocyte production, and inhibition of GSK3β by drugs can also block the differentiation of adipocytes. Further results found that the activation of Wnt signaling pathway can inhibit the differentiation of adipocytes by preventing the induced expression of PPARγ and CEBP / α. In contrast, overexpression of constitutive active AXIN or dominant negative TCF7L2 resulted in spontaneous differentiation of preadipocytes. It should be noted that the above-mentioned interventions all occurred in the very early ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027C12Q1/6888
CPCA01K67/0276A01K2217/075A01K2227/105A01K2267/0362C12Q1/6888C12Q2600/158
Inventor 宁光王计秋芦鹏刘瑞欣
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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