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Method for regulating CPEB1 gene expression by combining transcription factor CEBPB with CPEB1 gene promoter region

A gene promoter region, transcription factor technology, applied in the field of medicine and health

Active Publication Date: 2021-04-27
AFFILIATED HOSPITAL OF NANTONG UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

No new hypermethylated tumor suppressor genes and their corresponding upstream regulatory factors, such as transcription factors and methylation recognition factors, have been reported so far

Method used

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  • Method for regulating CPEB1 gene expression by combining transcription factor CEBPB with CPEB1 gene promoter region
  • Method for regulating CPEB1 gene expression by combining transcription factor CEBPB with CPEB1 gene promoter region
  • Method for regulating CPEB1 gene expression by combining transcription factor CEBPB with CPEB1 gene promoter region

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Embodiment Construction

[0053]In order to make the technical problems, technical solutions and advantages to solve the present invention more clearly, and will be described in detail below with reference to the accompanying drawings and specific examples.

[0054]The present invention provides an transcription factor CEBPB and TFCP2 competition binding to CPEB1 gene promoter regions to regulate CPEB1 gene expression, including the following steps:

[0055]S1, the transcription factor CEBPB can be combined with the CPEB1 gene promoter region by experiment, and the transcription factor CEBPB cannot be combined, which affects the chromatinability of the CPEB1 gene induced by the CPEB1 gene promoter region. Among them, the detailed steps of step (1) are:

[0056]S1.1, Double Lucinozyme Report Gene Analysis: Cloning CPEB1 promoter zone cloned into the PGL3-Basic carrier or PCPGL-Basic vector, obtaining a reported plasmid vector, the insertion fragment is verified by Sanger sequencing; confirm CCAA / T Enhanced binding p...

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Abstract

The invention provides a method for regulating CPEB1 gene expression by competitively combining transcription factors CEBPB and TFCP2 with a CPEB1 gene promoter region. The method comprises the following steps of S1, verifying through experiments that the transcription factor CEBPB can be combined with the CPEB1 gene promoter region, and when the CPEB1 gene promoter region is in a hypermethylation state, the transcription factor CEBPB cannot be combined, so that the chromatin accessibility of a CPEB1 gene is influenced and the CPEB1 gene expression is inhibited; and S2, verifying through experiments thatwhen the CPEB1 gene promoter region is in the hypermethylation state, the transcription factor TFCP2 can be combined with the CPEB1 gene promoter region, so that the combination of the transcription factor CEBPB is prevented. Experimental results show that the DNA hypermethylation state of the CPEB1 gene promoter region can enhance the binding activity of the transcription factor TFCP2, so that the promoter region cannot be combined with the CEBPB, and meanwhile, it is proved that the transcription factor TFCP2 is a new DNA methylation recognition factor.

Description

Technical field[0001]The present invention belongs to the field of medical and health, and more particularly to a method of adjusting CPEB1 gene expression in combination of transcription factor CEBPB and TFCP2 competition binding CPEB1 gene promoter region.Background technique[0002]The cytoplasmic poly adenosine element binding protein 1 (CPEB1) is a sequence-specific RNA binding protein that regulates polytenylation and translation of mRNA, which has been shown to be related to progression and metastasis of cancer. CPEB1 can directly target the 3'-UTR of Sirt1 (Sirtuin 1), control the length of the Poly (A) tail and inhibit its translation, and mediate the dryness of cancer cells in vivo. CPEB1 is reported to participate in the development of gastric cancer, breast cancer, glioma and liver cancer. However, the role of CPEB1 and its methylation state in colorectal cancer (CRC) is not clear.[0003]TFCP2 (Transcription Factor CP2, also known as TFCP2C, LBP1C, LSF, LSF1D, SEF) is a tra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N15/65C12N15/12C12Q1/6804C12Q1/6834
CPCC12N15/85C12N15/65C07K14/4702C12Q1/6804C12Q1/6834C12N2800/107C12Q2523/301C12Q2563/131C12Q2531/113C12Q2563/143C12Q2563/149
Inventor 丁伟峰濮伟霖邵可可张健锋鞠少卿马彦云
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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