A fluorescent probe for the specific detection of lipid droplets for distinguishing between normal cells and cancer cells and its application

A technology for fluorescent probes and normal cells, applied in the field of fluorescent probes, to achieve the effect of fast staining speed and low cytotoxicity

Active Publication Date: 2021-07-02
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention proposes a fluorescent probe for the specific detection of lipid droplets for distinguishing between normal cells and cancer cells and its application. The problem to be solved by the present invention is to provide a lipid droplet with ultra-high selectivity using coumarin as the parent Fluorescent probes and their use in specifically labeling or displaying lipid droplet morphology and distribution in living cells or tissues for imaging lipid droplets in cancer cells and tumor tissues without background noise

Method used

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  • A fluorescent probe for the specific detection of lipid droplets for distinguishing between normal cells and cancer cells and its application
  • A fluorescent probe for the specific detection of lipid droplets for distinguishing between normal cells and cancer cells and its application
  • A fluorescent probe for the specific detection of lipid droplets for distinguishing between normal cells and cancer cells and its application

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Experimental program
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Effect test

Embodiment 1

[0045] The structural formula of probe a is as follows:

[0046]

[0047] Probe a was prepared as follows: a mixture of diethyl oxaloxalate (1.818 g, 10 mM) and 3-(dimethylamino)phenol (1.3718 g, 10 mM) was stirred at 120° C. for 3 hours. After cooling, the reaction mixture was washed with CH 2 Cl 2 diluted and purified by silica gel column chromatography (CH 2 Cl 2 ), an orange solid (1.07 g, 45% yield) was obtained.

[0048]

Embodiment 2

[0050] The structural formula of probe b is as follows:

[0051]

[0052] Probe b was prepared as follows: a mixture of diethyl oxalate (1.818 g, 10 mM) and 3-(diethylamino)phenol (1.3718 g, 10 mM) was stirred at 120° C. for 3 hours. After cooling, the reaction mixture was washed with CH 2 Cl 2 diluted and purified by silica gel column chromatography (CH 2 Cl 2 ), an orange solid (1.07 g, 45% yield) was obtained.

[0053]

[0054] The application of the probe a prepared in embodiment 1:

[0055] 1. Changes in fluorescence intensity of probe a interacting with solvents of different polarities

[0056] Prepare a PBS (10mM) buffer solution with pH=7.4; weigh the probe, dissolve it in DMSO, and accurately prepare a 2mM probe storage solution; add 2mL of 1,4-dioxane and dichloro After methane, ethyl acetate, tetrahydrofuran, dimethyl sulfoxide, N,N-dimethylformamide, ethylene glycol, ethanol, acetonitrile, methanol and PBS buffer solution, add 4 μL of probe stock soluti...

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Abstract

The present invention provides a fluorescent probe for specifically detecting lipid droplets for distinguishing between normal cells and cancer cells and its application. The morphology of ground-labeled lipid droplets shows the distribution and application in living cells or tissues or in vivo, and can distinguish normal cells from cancer cells, normal tissues and tumor tissues through fluorescence.

Description

technical field [0001] The invention relates to the technical fields of organic small molecule fluorescent probes and biosensing, in particular to a fluorescent probe for specifically detecting lipid droplets for distinguishing normal cells and cancer cells and its application. Background technique [0002] Lipid droplets (LDs) have attracted increasing attention due to their special structures and physiological functions. LDs, as lipid-rich subcellular organelles, are widely found in eukaryotic cells. LDs consist of a neutral lipid core through which a monolayer of phospholipids mounts associated proteins. In addition to serving as energy storage, LDs are also involved in various physiological processes, including increased cell activation, migraine, apoptosis, etc. Accumulating evidence indicates that the abnormality of LDs is related to the development of cancer. Therefore, research on favorable tumor markers is particularly important for basic and clinical research. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/18C09K11/06G01N21/64
CPCC07D311/18C09K11/06C09K2211/1088G01N21/6428
Inventor 李朝辉孙远强魏星王霞屈凌波
Owner ZHENGZHOU UNIV
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