Method for evaluating effects of insecticides in preventing and controlling tomato whiteflies and tomato yellow leaf curl virus (TYLCV) indoors

Abstract

The invention relates to a method for evaluating effects of insecticides in preventing and controlling tomato whiteflies and a tomato yellow leaf curl virus (TYLCV) indoors. The method comprises the steps that each chemical, each concentration and blank controls of to-be-evaluated insecticides are each set as one treatment, and each treatment has three replicates or more; each replicate takes equivalent tomato seedlings during the true leaf period, each treatment uniformly sprays corresponding equivalent liquid chemicals to the tomato seedlings, and after the liquid chemicals are applied, thetomato seedlings are put in an artificial climate chamber for cultivation, so that the liquid chemicals are fully absorbed; after the liquid chemicals are applied, each replicate is inoculated with equivalent whitefly adults carrying the TYLCV, and the number of survival whitefly adults carrying the TYLCV on the tomato seedlings of each replicate in different time periods after inoculation performed with the adults is observed; and after the replicates are each inoculated with the equivalent whitefly adults carrying the TYLCV for 14 days, TYLCV disease symptoms are surveyed, the TYLCV diseaselevel of each treatment is determined, and disease indexes and the control effect are calculated. Under the constant conditions, the types and the use concentrations of the insecticides capable of effectively controlling the whiteflies and a tomato yellow leaf curl disease spread by the whiteflies are evaluated and screened.

Description

technical field [0001] The invention relates to the technical field of plant protection, and more specifically relates to a method for indoor evaluation of the effect of insecticides on controlling tomato whitefly and TYLCV. Background technique [0002] Bemisia tabaci and tomato yellow leaf curl virus disease (TYLCD), which it transmits, have caused serious damage in recent years. The use of insecticides to inhibit the transmission of viruses between plants is a common method for the prevention and control of this combination of diseases and insect pests. It is limited to the actual application in the field. The application process is affected by various agricultural operation factors, meteorological factors, environmental factors, etc., and the application effect is not stable enough to accurately judge the control effect of insecticides on viral diseases. [0003] Therefore, the problems existing in the prior art need to be further improved and developed. Contents of th...

AI Technical Summary

Problems solved by technology

It is limited to the actual application in the field. During the application process, it is affected by various agricultural operation factors, meteorological factors, environmental factors, etc., and the application effect is not stable enough to accurately judge the control effect of insecticides on viral diseases.

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