Ultrasonic preparation method for carotenoid geometrical isomer

A technology of carotene and geometric isomerization, applied in chemical instruments and methods, purification/separation of hydrocarbons, chemical/physical/physicochemical processes using energy, etc., can solve the problem of undiscovered carotenoid geometric isomers and Reporting and other issues, to achieve the effect of easy isomerization and, control isomerization and, easy degradation ratio

Active Publication Date: 2010-10-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, through the search of domestic and foreign patents and literature, we have not found

Method used

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Effect test

Embodiment 1

[0024] The ultrasonic preparation method of embodiment 1, beta-carotene geometric isomer, carries out following steps successively:

[0025] 1), weigh 5 mg of all-trans β-carotene; use dichloromethane as solvent, prepare 10.0 μg / ml β-carotene solution with a 500 ml volumetric flask; that is, each ml of β-carotene solution contains 10.0 μg of all-trans β-carotene;

[0026] 2) Take 30ml of β-carotene solution and add it to the brown sample glass tube, and use the probe-type pulsed ultrasonic wave (20khz, 10mm probe) to process it. During the process, use a low-temperature constant temperature bath to control the temperature. The specific settings are as follows: insert the probe 1cm below the liquid , the liquid level is kept at 6cm, the pulse time is 2s, the duty cycle is 66.7%, the temperature is -5°C, and the sound intensity is 302.55W / cm 2 , processing time 10 minutes.

[0027] 3), the product of step 2) gained is carried out chromatographic separation; Concrete steps and ...

Embodiment 2

[0033] Embodiment 2, the ultrasonic preparation method of β-carotene geometric isomer, carries out following steps successively:

[0034] 1), weigh 5 mg of all-trans β-carotene; use dichloromethane as solvent, prepare 10.0 μg / ml β-carotene solution with a 500 ml volumetric flask; that is, each ml of β-carotene solution contains 10.0 μg of all-trans Formula β-carotene;

[0035] 2) Take 30ml of β-carotene solution and add it to the brown sample glass tube, and use the probe-type pulsed ultrasonic wave (20khz, 10mm probe) to process it. During the process, use a low-temperature constant temperature bath to control the temperature. The specific settings are as follows: insert the probe 1cm below the liquid , the liquid level is kept at 6cm, the pulse time is 2s, the duty cycle is 50%, the temperature is 25°C, and the sound intensity is 60.51W / cm 2 , processing time 20 minutes.

[0036] 3), the product of step 2) gained is carried out chromatographic separation, concrete steps an...

Embodiment 3

[0042] Embodiment 3, the ultrasonic preparation method of lycopene geometric isomer, carries out following steps successively:

[0043] 1), weigh 5 mg of all-trans lycopene (lycopene); use dichloromethane as solvent, and use a 500ml volumetric flask to prepare a 10.0 μg / ml lycopene solution; that is, each ml of lycopene solution contains 10.0 μg of all-trans tomato red pigment;

[0044] 2) Take 30ml of lycopene solution and add it to the brown sample glass tube, and use probe-type pulsed ultrasonic wave (20khz, 10mm probe) to process. During the process, use a low-temperature constant temperature bath to control the temperature. The specific settings are as follows: insert the probe 1cm below the liquid, The liquid level is kept at 6cm, the pulse time is 2s, the duty cycle is 50%, the temperature is 5°C, and the sound intensity is 60.51W / cm 2 , the processing time is 8 minutes.

[0045] 3), the product of step 2) gained is carried out chromatographic separation, concrete ste...

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Abstract

The invention discloses an ultrasonic preparation method for a carotenoid geometrical isomer, which comprises the following steps: (1) taking all-trans carotenoid and methylene chloride as a raw material and a solvent respectively so as to prepare solution; (2) putting the above solution into a container and using 20 khz probe-type pulse ultrasonic wave to process, wherein the processing procedure comprises the following steps: inserting the probe into undersurface of the solution for 1cm; keeping the height liquid level being within the range of 5-8cm to process for 5-20 minutes under the contions of 2-5s of pulse time, 50%-90% of duty cycle, -5-25 DEG G of temperature and 60.51-544.59 W/cm2 of sound intensity; and (3) using a C30 carotenoid chromatographic column to carry out routine chromatograph separation on product obtained by step (2) to obtain the carotenoid geometrical isomer; in addition, step (1) and step (2) are operated in the condition without sunlight. The carotenoid geometrical isomers with different types and quantities can be obtained effectively by the method.

Description

technical field [0001] The invention relates to the field of food processing, in particular to an ultrasonic preparation method of carotenoid geometric isomers, which can be used for structure-effect analysis of active ingredients in functional foods. Background technique [0002] Carotenoids have a variety of biological activities, some of which are important components of human tissues and organs, such as lutein is a component of the macula, insufficient intake may lead to age-related macular degeneration (AMD), and Age-related macular degeneration is the main disease that causes vision loss in the elderly in western countries (Perera, C.O., & Yen, G.M. Functional properties of carotenoids in human health. International Journal of Food Properties, 2007, 10(2): 201-230). Some carotenoids are the source of micronutrient VA, and there are still 200 million children suffering from VA deficiency in the world (Chen Junshi, Han Fanfan. Analysis of "New Nutrition Science" [J]. Nut...

Claims

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Application Information

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IPC IPC(8): C07C403/24C07C11/21C07C7/00B01J19/10
Inventor 孙玉敬叶兴乾陈健初刘东红吴丹张献忠
Owner ZHEJIANG UNIV
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