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Human malignant phyllodes tumor cell line LJ-0429 and applications thereof

A LJ-0429, malignant tumor technology, applied in the field of human malignant phyllodes tumor cell line LJ-0429, can solve problems such as limited value, and achieve the effect of short establishment time and stable biological heredity

Active Publication Date: 2020-04-17
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing molecular markers are also of limited value in predicting tumor biological behavior

Method used

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  • Human malignant phyllodes tumor cell line LJ-0429 and applications thereof

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Experimental program
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Effect test

Embodiment 1

[0018] The human phyllodes tumor cell line LJ-0429 of the present invention is obtained by the following method:

[0019] (1) Specimen collection and storage methods: After the complete mammary gland phyllodes tumor has been completely removed, the tumor tissue is cut in the middle with a sterile scalpel, and the vigorously proliferating tissue is taken in DMEM medium. The collection of specimens is carried out under the guidance of the surgeon and the pathologist to prevent the impact on the diagnosis of the pathology report; when the specimens are not used for follow-up operations, they should be stored in DMEM medium at 4°C, and the specimens should be used within 24 hours as much as possible .

[0020] (2) Primary culture: the tissue was washed 3 times with PBS, cut mechanically until it was as small as possible, digested with type III collagenase (1mg / ml, Worthington) and added to a 50ml centrifuge tube containing DMEM / F12, protected from light with tinfoil, 37°C, 180 rp...

Embodiment 2

[0035] Embodiment 2 detects the expression situation of virus

[0036] Q-PCR (quantitative real-time polymerase chain reaction, referred to as quantitative PCR) was used to detect the expression level of the introduced immortalization gene SV40T in the LJ-0429 cell line. Wherein, the gene primers used are shown in Table 1 below.

[0037] Table 1 Primer base sequence

[0038] Forward (forward primer) Reverse (reverse primer) GAPDH AGAGCCTCGAGGAGAAGTTCC (SEQ ID NO. 1) ACTAGGGAGTCAAGGACGGG (SEQ ID NO. 2) SV40T GCCAGTACCGTGCCTTATCC (SEQ ID NO. 3) GAACCACTAGGCCCATAACCA (SEQ ID NO. 4)

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Abstract

The invention discloses a human malignant phyllodes tumor cell line LJ-0429, which is preserved in China Center for Type Culture Collection, wherein the preservation address is Wuhan University, Wuhan, China, and the preservation number is C2018234. The invention also discloses applications of the human malignant phyllodes tumor cell line LJ-0429 as a cell model in research of tumor occurrence anddevelopment mechanism and in screening of antitumor drugs. The human malignant phyllodes tumor cell line LJ-0429 is established from the source of Chinese people, the line establishment time is short, the biological heredity is stable, no human malignant phyllodes tumor cell line exists on the market at present, and by using the phyllodes tumor cell line as a research model, great help can be provided for knowing the pathogenesis of Chinese malignant phyllodes tumor patients.

Description

technical field [0001] The invention relates to the technical field of animal cell lines, in particular to a human malignant phyllodes tumor cell line LJ-0429 and its application Background technique [0002] Phylodes tumor of the breast is a rare breast tumor, accounting for about 1% of breast tumors. This tumor grows rapidly and often presents as a huge mass; it is divided into benign, borderline, and malignant histologically; even Benign phyllodes tumors can also recur, and malignant phyllodes tumors can also undergo hematogenous metastasis. The therapeutic effects of chemotherapy and radiotherapy on phyllodes tumors are uncertain. The current treatment that can reduce the probability of recurrence and metastasis is extended surgical resection, but even with extended surgical resection, the local recurrence rate of phyllodes tumors is still as high as 8-36% %, the hematogenous metastasis rate of malignant phyllodes tumors is as high as 22%. [0003] Currently, the mecha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/02C12R1/91
CPCC12N5/0693G01N33/5011C12N2503/02G01N2500/10Y02A50/30
Inventor 宋尔卫聂燕黄红颜
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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