Terahertz metamaterial sensor for cell migration and detection method using same

Abstract

The terahertz metamaterial sensor comprises a substrate, a terahertz metamaterial structure sensing unit and a polydimethylsiloxane fence used for limiting a cell growth area, the terahertz metamaterial structure sensing unit is formed on the substrate, and a splitting ring and two circular ring alignment marks which are arranged periodically are formed on the terahertz metamaterial structure sensing unit; and the polydimethylsiloxane fence is positioned on the substrate and comprises a polydimethylsiloxane square sheet with a circular hollow middle part and a solid polydimethylsiloxane cylindrical sheet with an outer diameter aligned with the alignment mark of a circular ring of an inner ring, and the inner diameter of a hollow circle of the polydimethylsiloxane square sheet is aligned with the alignment mark of a circular ring of an outer ring of the terahertz metamaterial structure sensing unit. The sensor is simple in preparation process, high in detection speed and small in required cell quantity, and a rapid and reliable method is provided for the detection of cell migration.

Description

technical field [0001] The invention relates to the technical field of terahertz metamaterial biosensing, in particular to a terahertz metamaterial sensor for cell migration and a detection method using the same. Background technique [0002] Cell migration experiment is one of the commonly used experimental methods in cell biology, especially in the field of tumor biology research, and it is an important method to evaluate the stimulating effect of stimulating factors on cells. Traditional cell migration detection experiments are time-consuming and require the experimenter to manually set the "cell wound". When observing, the distance needs to be measured under a microscope, which takes a long time and is troublesome to operate. With the development of microfluidic technology, some cells are used to detect cell migration. Advanced microfluidic chips are gradually developed, but the high flow rate of microfluidics and the processing of microstructures require precise control...

AI Technical Summary

Problems solved by technology

Terahertz detection has the problem of "water sensitivity". The current solution is to use light-transparent agents or introduce microfluidic chip technology. Most light-transparent agents are organic solvents and are incompatible with hydrophilic cells; PDMS microfluidic chips will Brings a lot of THz light absorption, and increases the complexity of the experiment

Without the microfluidic chip, the cell growth area cannot be limited

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