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Application of sodium hypochlorite in separation and bleaching of upper jawbone of periodontitis model mouse

A sodium hypochlorite, model mouse technology, applied in the preparation of test samples, material inspection products, biological tests, etc., can solve problems such as low efficiency and unclean removal of jaw soft tissue, achieve easier coloring, improve digestion efficiency, A wide range of effects

Pending Publication Date: 2020-06-12
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing technology is to directly use tweezers to mechanically remove the soft tissue of the jawbone or use hydrogen peroxide for overnight bleaching, which has problems such as low efficiency and unclean removal of the jawbone soft tissue
[0004] Therefore, it is urgent to provide an application of sodium hypochlorite in the separation and bleaching of the maxilla in periodontitis model mice to solve the problems of low efficiency and unclean jawbone soft tissue removal

Method used

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  • Application of sodium hypochlorite in separation and bleaching of upper jawbone of periodontitis model mouse
  • Application of sodium hypochlorite in separation and bleaching of upper jawbone of periodontitis model mouse

Examples

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Embodiment

[0025] This example provides an application of sodium hypochlorite in the separation and bleaching of the maxilla in periodontitis model mice.

[0026] In this embodiment, the application includes: soaking the jawbone in sodium hypochlorite and then staining it with methylene blue.

[0027] In this embodiment, before the staining step, it also includes the step of dissecting the jaws of the periodontitis model mice and soaking them in phosphate-buffered saline (PBS buffer).

[0028] In this embodiment, before the staining step or after the buffer soaking step, a step of stripping the attached periodontal tissue of the jawbone under a microscope is also included.

[0029] In one embodiment of the present invention, a kind of sodium hypochlorite is provided in the periodontitis model mouse maxillary separation and the application of bleaching, comprising:

[0030] The step of dissecting the jawbone of the periodontitis model mouse and soaking it with PBS buffer solution;

[00...

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Abstract

The invention discloses an application of sodium hypochlorite in separation and bleaching of upper jawbone of a periodontitis model mouse. The application comprises the following steps: soaking the jawbone in sodium hypochlorite, and dyeing the jawbone with methylene blue. According to the invention, sodium hypochlorite is adopted to improve the digestion efficiency of residual tissues attached tothe jawbone and play a role in bleaching, so that the boundary between the jawbone and teeth is displayed more clearly, and the periodontal attachment loss value is convenient to measure.

Description

technical field [0001] The invention relates to the technical field of periodontitis treatment, in particular to the application of sodium hypochlorite in the separation and bleaching of the maxilla of periodontitis model mice. Background technique [0002] Chronic periodontitis (CP) is a destructive inflammatory disease of tooth supporting soft and hard tissues, and it is the main cause of tooth loss in adults. According to the fourth national oral health epidemiological survey, the periodontal health rates of the 35-44, 55-64 and 65-75 age groups were only 9.1%, 5.0% and 9.3%, respectively. Therefore, basic experiments to explore the mechanism of periodontitis are necessary. An important measure of the severity of chronic periodontitis is loss of periodontal attachment. The periodontal attachment loss value was measured by measuring the distance from the enamel-cementojunction (CEJ) to the alveolar bone crest (ABC) to understand the degree of alveolar bone resorption. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30G01N1/34G01N33/48
CPCG01N1/28G01N1/30G01N1/34G01N33/48
Inventor 陆海霞马晓欣王用利
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE