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35 results about "Residual Tissues" patented technology

Method for efficientlyobtaining mesenchymal stem cells from umbilical cord tissues

The invention relates to amethod for efficientlyobtaining mesenchymal stem cells from umbilical cord tissues and aims at solving the problems of the long obtaining time and low efficiency of an existing method. The method comprises the steps that primary isolation of umbilical cord mesenchymal stem cells is performed: cleaned and cut umbilical cord tissue blocks are predigested by using trypsin and then digestive juice is removed, repeated flushing is performed with DPBS, then digestion is performed on a water bath shaker of 37 DEG C by using collagenase I digestive juice, a complete culture solution is added to the filtrate filtered by a cell strainer to terminate digestive centrifugal separation so as to obtain primary mesenchymal stem cell; residual tissue blocks are collected after filtration, and re-digestion is performed to obtain primary mesenchymal stem cells; the mesenchymal stem cells are inoculated in the complete culture solution, and standing and culture is performed in 5% CO2 culture box at the temperature of 37 DEG C; a culture solution is replaced for one time at intervals of several days; culture multiplication is performed: when the primary cells grow to be 80% and converge, TrypLE heavy suspension cells are subjected to culture multiplication in a serum-free culture solution. The method has the advantages of efficiently obtaining mesenchymal stem cells, and the obtained mesenchymal stem cells have highcell activity and differentiation potential.
Owner:中卫华医(北京)生物科技有限公司 +1

Multifunctional cutting-up bench for pathological diagnosis

The invention provides a multifunctional cutting-up bench for pathological diagnosis, comprising a support frame, a cutting-up bench surface, a functional frame, and a top plate, wherein the support frame is of a rectangle shape; the cutting-up bench surface is arranged on the upper surface of the support frame; the cutting-up bench surface is provided with the functional frame at one side with a long edge; the upper end of the functional frame is provided with the top plate; the lower end of the support frame is divided into a plurality of spaces by utilizing clapboards; a left space is of an apparatus room; apparatuses for sampling are placed in the apparatus room; a right space is of a storage room; the middle part of the functional frame is provided with a storage rack; the width of the storage rack is smaller than that of the cutting-up bench surface. The multifunctional cutting-up bench for pathological diagnosis provided by the invention is simple in structure, convenient to use, is capable of meeting multiple functions of cutting up, cleaning and disinfection of a work area, waste gas treatment, residual tissue smashing, cleaning and drying of hands, fixation and cutting up of medical bone tissues, and the like at the same time, greatly improves the work environment of pathologic cutting works, and improves the work efficiency of workers.
Owner:陈海霞

Bipolar radiofrequency intestinal tract anastomat and working method

The invention relates to a bipolar radiofrequency intestinal tract anastomat and a working method. The anastomat comprises a near-end structure, a far-end structure and a radiofrequency energy generator, wherein the near-end structure is placed at the near end of an intestinal tract; the far-end structure is divided into a head portion and a handle portion, is located at the far end of the intestinal tract, and connects a positive pole with a negative pole of the radiofrequency energy generator through a guide wire inside the handle portion; and after the near-end structure is in butt joint with the far-end structure, the far-end structure becomes the negative pole, and a metal circular ring of the near-end structure is the positive pole. The intestinal tract is respectively sleeved on the near-end structure and the far-end structure, a portion needing anastomosis is collected to be attached to the metal circular ring of the near-end structure and a metal circular ring of the far-end structure, after sleeving, the metal circular ring of the near-end structure and the metal circular ring of the far-end structure form a circuit with the positive pole and the negative pole, intestinal tract anastomosis is completed after energization, after electricity is cut off, an internal push-type cutter is triggered through a switch of the handle portion, and residual tissue in the intestinal tract is cut off to guarantee smoothness of the intestinal tract. The anastomosis portion is continuous, operation time is short, no foreign matters are left in the intestinal tract after an operation, and safety of the anastomosis process is guaranteed.
Owner:UNIV OF SHANGHAI FOR SCI & TECH

Nose epithelial stem cell culture method and nose epithelial stem cell proliferation medium

ActiveCN108753681AMaintain proliferative capacityMaintain differentiation capacityEpidermal cells/skin cellsArtificial cell constructsPenicillinNose
The invention discloses a nose epithelial stem cell culture method which comprises the following steps: 1) laying nourishing layer cells into a culture dish; 2) separating primary cells of nose epithelial stem cells from nasal mucosa; 3) laying the primary cells of the nose epithelial stem cells on the nourishing layer cells, replacing a medium by a nose epithelial stem cell proliferation medium,and removing off residual tissue cells; 4) when the nose epithelial stem cells are proliferated to a density of 80%, carrying out trypsinization, and carrying out continuous passage amplification; 5)transferring the nose epithelial stem cells into a gas-liquid phase culture system and a PneumaCult-ALI differentiation medium, and carrying out differentiation culture. The invention further discloses a nose epithelial stem cell proliferation medium which comprises the following components: DMEM/F12, fetal calf serum, penicillin, streptomycin, insulin, an epidermal growth factor, hydrocortisone,3,3',5-triiodo-L-sodium adenoglycosine and an ROCK inhibitor. By adopting the method, a key application base is provided for transformation application of clinical transplanting of the nose epithelialstem cells.
Owner:THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV

Neural stem cell medium change system and medium change method for neural stem cell culture

The invention relates to a cell culture medium change method, in particular to a neural stem cell medium change system and a medium change method for neural stem cell culture. The neural stem cell medium change system comprises a cell interceptor, a waste storage bottle, a sterile air bottle and an air pump in sequential connection, the cell interceptor is formed by connection of a titanium rod type filter head and a stainless steel pipe, and aperture of the titanium rod type filter is 4.0-5.0 micrometers. Alternatively, the neural stem cell medium change system structurally comprises the cell interceptor, a syringe and a connection pipe, the cell interceptor is made of the stainless steel pipe with a stainless steel filter screen inside, and mesh diameter of the stainless steel filter screen is 4.0-5.0 micrometers. The neural stem cell medium change system and the medium change method for neural stem cell culture have the advantages that medium change time in a neural stem cell culture process can be shortened, loss of precious neural stem cells in a medium change process is prevented, residual tissue fragments in a culture medium can be cleared away, lives of cells cultured in vitro are prolonged, and labor saving, productivity improvement and convenience for industrial production are realized.
Owner:ZHEJIANG ORIGIN BIOTECH

Traditional Chinese medicine pill for treating residual tissues in uterine cavity after miscarriage

The invention relates to a traditional Chinese medicine pill for treating residual tissues in the uterine cavity after miscarriage, and belongs to the technical field of Chinese herbal medicine. The traditional Chinese medicine pill is prepared by the following steps: crushing peach kernel, safflower, angelica sinensis, ligusticum wallichii, radix paeoniae alba, radices trichosanthis, Chinese honey locust, spina gleditsiae, curcuma zedoary, rhizoma sparganii, leech, dianthus superbus, trogopterus dung, quispualis indica, seaweed, raw oyster, raw chlorite schist, leonurus, folium artemisiae argyi, radix aconiti lateralis preparata, medicinal cyathula root, achyranthes root and other traditional Chinese medicinal materials, and sieving powder by using a 100-mesh sieve. The traditional Chinese medicine pill has the functions of resolving hard lumps, breaking blood and eliminating aggregation, removing stasis and relieving pain, removing the fetus to benefit the mother and the like, can prompt continuous uterine contraction to remove blood clots, adhesions and the like in the uterine cavity, is good in curative effect, quick to become effective and convenient to take, is safe and reliable, has an effect of treating both symptoms and root causes, overcomes the shortcomings of existing medicine or technology for treating residual tissues in uterine cavity after miscarriage, is noninvasive, greatly relieves the pain of patients and has positive significance for treating residual tissues in uterine cavity after miscarriage.
Owner:YANGTZE UNIVERSITY

Pesticide capable of preventing and treating seedling-period damping-off of chilies

The invention relates to pesticide capable of preventing and treating seedling-period damping-off of chilies. The pesticide is prepared from the following components: mimoselike rosewood leaves, borax, ailanthus altissima leaves, sceptrocnide macrostachya maxim leaves, sodium chloride, washing powder, chicken bile, sodium hypochlorite and clean water. The pesticide disclosed by the invention is green and free from pollution, and pollution to the environment, and damages to human bodies, caused by the fact that a lot of chemical agents are used, are avoided; the half-year-cold mimoselike rosewood leaves, three-year-old to four-year-old ailanthus altissima leaves and two-year-old sceptrocnide macrostachya maxim leaves are treated by an enzymatic hydrolysis solution, mixed extract has an extremely great effect on inhibition of germination of pathogenic bacterium oospores, and is matched with a chicken bile, borax and washing powder mixture treated by sodium hypochlorite, so that the pesticide effect can be obviously improved, and sporangia and planoconidia, generated by diseased parts of young seedling, are killed; and the pesticide has inhibition and killing effects on mycelia which have saprogenesis in diseased residual tissues or humic matters, and can prevent a condition that the young seedlings are infected by sporangia and planoconidia, which are generated in the next spring. With the adoption of the pesticide, the prevention effect on the seedling-period damping-off of the chilies is up to 96.2% and the treatment effect is up to 96.1%.
Owner:ANQING LYUYUAN AGRI TECH CO LTD

Bipolar radiofrequency intestinal tract anastomat and working method

The invention relates to a bipolar radiofrequency intestinal tract anastomat and a working method. The anastomat comprises a near-end structure, a far-end structure and a radiofrequency energy generator, wherein the near-end structure is placed at the near end of an intestinal tract; the far-end structure is divided into a head portion and a handle portion, is located at the far end of the intestinal tract, and connects a positive pole with a negative pole of the radiofrequency energy generator through a guide wire inside the handle portion; and after the near-end structure is in butt joint with the far-end structure, the far-end structure becomes the negative pole, and a metal circular ring of the near-end structure is the positive pole. The intestinal tract is respectively sleeved on the near-end structure and the far-end structure, a portion needing anastomosis is collected to be attached to the metal circular ring of the near-end structure and a metal circular ring of the far-end structure, after sleeving, the metal circular ring of the near-end structure and the metal circular ring of the far-end structure form a circuit with the positive pole and the negative pole, intestinal tract anastomosis is completed after energization, after electricity is cut off, an internal push-type cutter is triggered through a switch of the handle portion, and residual tissue in the intestinal tract is cut off to guarantee smoothness of the intestinal tract. The anastomosis portion is continuous, operation time is short, no foreign matters are left in the intestinal tract after an operation, and safety of the anastomosis process is guaranteed.
Owner:UNIV OF SHANGHAI FOR SCI & TECH
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