Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method capable of enhancing immunoregulation capacity of umbilical cord mesenchymal stem cells

A technology of stem cells and regulation ability, which is applied in the field of enhancing the immune regulation ability of umbilical cord mesenchymal stem cells, and can solve the problem of poor immune regulation ability of mesenchymal stem cells.

Pending Publication Date: 2018-10-16
SICHUAN NEO LIFE STEM CELL BIOTECH
View PDF11 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the conventional medium used for the cultivation of mesenchymal stem cells is the medium containing fetal bovine serum or serum-free medium such as LONZA, but the immunoregulatory ability of the mesenchymal stem cells cultured by them is not strong, and needs to be further improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method capable of enhancing immunoregulation capacity of umbilical cord mesenchymal stem cells
  • Method capable of enhancing immunoregulation capacity of umbilical cord mesenchymal stem cells
  • Method capable of enhancing immunoregulation capacity of umbilical cord mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 Serum-free culture method of umbilical cord mesenchymal stem cells of the present invention

[0030] Use the working concentration of CELLstart CTS matrix to coat a plastic petri dish with a diameter of 100 mm, and coat at 37°C for 1 hour. After the coating is completed, remove the excess liquid, and the coated petri dish is ready for use;

[0031] The umbilical cords were taken from healthy puerperas with full-term vaginal delivery or caesarean section. After aseptic collection, they were stored in phosphate buffer solution at 4°C and processed within 24 hours; the umbilical cord was taken out in a sterile operating table and washed repeatedly with phosphate buffer solution until after cleaning. The liquid is transparent;

[0032] Cut the cleaned umbilical cord into 2 cm pieces, pull out the arteries and veins and cut off the amniotic tissue around the umbilical cord, cut the remaining tissue into small pieces of 0.5 to 1 cubic millimeter, mix 2 ml (mix 2 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a method capable of enhancing the immunoregulation capacity of umbilical cord mesenchymal stem cells. The method comprises the following steps: (1) taking umbilical cord, carrying out washing, cutting the umbilical cord to be short, removing the amniotic membrane tissue on the peripheries of the blood vessel and the umbilical cord, cutting the residual tissue into small blocks, and carrying out culturing by adopting the StemPro MSC SFM CTS complete culture medium; and (2) when the cell merging rate achieves 25-30 %, carrying out digestion by adopting the pancreatin substitute CTS TrypLE Select Enzyme, carrying out washing, and collecting a cell suspension. For the mesenchymal stem cells treated by adopting the method provided by the invention, the secretion level ofthe immunoregulation related factors is high, the inhibiting effect for single karyocyte is great and is obviously higher than the effect of the conventional serum-containing culture medium, meanwhile, no influences are caused to the other properties of the mesenchymal stem cells, such as the differentiation capability, and thus the application prospect is good.

Description

technical field [0001] The invention relates to a method for enhancing the immune regulation ability of umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells are a type of cells with self-renewal, high-speed proliferation, and multi-directional differentiation potential, which are widely used in the field of regenerative medicine. At present, mesenchymal stem cells have been isolated from various tissues including umbilical cord. Since umbilical cord tissue has the characteristics of convenient collection and large quantity, there are no ethical problems, so umbilical cord mesenchymal stem cells have gradually become a popular choice for scientific research and medical workers. research hotspots. [0003] Umbilical cord mesenchymal stem cells are a kind of stem cells that exist in umbilical cord Wharton's jelly and perivascular tissue. They not only maintain the biological characteristics of mesenchymal stem cells, but also have more P...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/0775A01N1/02
CPCA01N1/0221C12N5/0037C12N5/0665C12N2509/00
Inventor 杨超陈育李凡李文娴陈强
Owner SICHUAN NEO LIFE STEM CELL BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com