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Method capable of enhancing immunoregulation capacity of umbilical cord mesenchymal stem cells

A technology of stem cells and regulation ability, which is applied in the field of enhancing the immune regulation ability of umbilical cord mesenchymal stem cells, and can solve the problem of poor immune regulation ability of mesenchymal stem cells.

Pending Publication Date: 2018-10-16
SICHUAN NEO LIFE STEM CELL BIOTECH
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  • Claims
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Problems solved by technology

[0006] At present, the conventional medium used for the cultivation of mesenchymal stem cells is the medium containing fetal bovine serum or serum-free medium such as LONZA, but the immunoregulatory ability of the mesenchymal stem cells cultured by them is not strong, and needs to be further improved

Method used

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  • Method capable of enhancing immunoregulation capacity of umbilical cord mesenchymal stem cells
  • Method capable of enhancing immunoregulation capacity of umbilical cord mesenchymal stem cells
  • Method capable of enhancing immunoregulation capacity of umbilical cord mesenchymal stem cells

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Embodiment 1

[0029] Embodiment 1 Serum-free culture method of umbilical cord mesenchymal stem cells of the present invention

[0030] Use the working concentration of CELLstart CTS matrix to coat a plastic petri dish with a diameter of 100 mm, and coat at 37°C for 1 hour. After the coating is completed, remove the excess liquid, and the coated petri dish is ready for use;

[0031] The umbilical cords were taken from healthy puerperas with full-term vaginal delivery or caesarean section. After aseptic collection, they were stored in phosphate buffer solution at 4°C and processed within 24 hours; the umbilical cord was taken out in a sterile operating table and washed repeatedly with phosphate buffer solution until after cleaning. The liquid is transparent;

[0032] Cut the cleaned umbilical cord into 2 cm pieces, pull out the arteries and veins and cut off the amniotic tissue around the umbilical cord, cut the remaining tissue into small pieces of 0.5 to 1 cubic millimeter, mix 2 ml (mix 2 ...

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Abstract

The invention discloses a method capable of enhancing the immunoregulation capacity of umbilical cord mesenchymal stem cells. The method comprises the following steps: (1) taking umbilical cord, carrying out washing, cutting the umbilical cord to be short, removing the amniotic membrane tissue on the peripheries of the blood vessel and the umbilical cord, cutting the residual tissue into small blocks, and carrying out culturing by adopting the StemPro MSC SFM CTS complete culture medium; and (2) when the cell merging rate achieves 25-30 %, carrying out digestion by adopting the pancreatin substitute CTS TrypLE Select Enzyme, carrying out washing, and collecting a cell suspension. For the mesenchymal stem cells treated by adopting the method provided by the invention, the secretion level ofthe immunoregulation related factors is high, the inhibiting effect for single karyocyte is great and is obviously higher than the effect of the conventional serum-containing culture medium, meanwhile, no influences are caused to the other properties of the mesenchymal stem cells, such as the differentiation capability, and thus the application prospect is good.

Description

technical field [0001] The invention relates to a method for enhancing the immune regulation ability of umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells are a type of cells with self-renewal, high-speed proliferation, and multi-directional differentiation potential, which are widely used in the field of regenerative medicine. At present, mesenchymal stem cells have been isolated from various tissues including umbilical cord. Since umbilical cord tissue has the characteristics of convenient collection and large quantity, there are no ethical problems, so umbilical cord mesenchymal stem cells have gradually become a popular choice for scientific research and medical workers. research hotspots. [0003] Umbilical cord mesenchymal stem cells are a kind of stem cells that exist in umbilical cord Wharton's jelly and perivascular tissue. They not only maintain the biological characteristics of mesenchymal stem cells, but also have more P...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A01N1/02
CPCA01N1/0221C12N5/0037C12N5/0665C12N2509/00
Inventor 杨超陈育李凡李文娴陈强
Owner SICHUAN NEO LIFE STEM CELL BIOTECH
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