Neural stem cell medium change system and medium change method for neural stem cell culture

Abstract

The invention relates to a cell culture medium change method, in particular to a neural stem cell medium change system and a medium change method for neural stem cell culture. The neural stem cell medium change system comprises a cell interceptor, a waste storage bottle, a sterile air bottle and an air pump in sequential connection, the cell interceptor is formed by connection of a titanium rod type filter head and a stainless steel pipe, and aperture of the titanium rod type filter is 4.0-5.0 micrometers. Alternatively, the neural stem cell medium change system structurally comprises the cell interceptor, a syringe and a connection pipe, the cell interceptor is made of the stainless steel pipe with a stainless steel filter screen inside, and mesh diameter of the stainless steel filter screen is 4.0-5.0 micrometers. The neural stem cell medium change system and the medium change method for neural stem cell culture have the advantages that medium change time in a neural stem cell culture process can be shortened, loss of precious neural stem cells in a medium change process is prevented, residual tissue fragments in a culture medium can be cleared away, lives of cells cultured in vitro are prolonged, and labor saving, productivity improvement and convenience for industrial production are realized.

Description

technical field [0001] The invention relates to a method for replacing cell culture fluid, in particular to a neural stem cell fluid replacement system and a fluid replacement method for neural stem cell culture. Background technique [0002] Mass culture of human neural stem cells is mainly used to prevent and treat neurodegenerative diseases, such as Parkinson's disease, Alzheimer's disease, spinal cord injury and other diseases. Usually, during the culture of neural stem cells, the medium needs to be changed regularly to remove part of the metabolic waste fluid, and then add an equal amount of fresh nutrient solution, so as to ensure the continued division and growth of neural stem cells. It is understood that one of the most common methods at home and abroad is the direct aspiration method and the centrifugation method, that is, the cell culture flask is erected first, and after the neural stem cell spheres are precipitated, the supernatant is gently aspirated, and then ...

AI Technical Summary

Problems solved by technology

It is understood that one of the most common methods at home and abroad is the direct aspiration method and the centrifugation method, that is, the cell culture flask is erected first, and after the neural stem cell spheres are precipitated, the supernatant is gently aspirated, and then an equal amount of fresh culture medium is added. It was determined that the removed supernatant contained some single neural stem cells and small neural stem cell spheres, which directly caused the loss of the number of neural stem cells during the culture process.

The other method is the centrifugation method. First, the cell culture bottle is erected. After the neural stem cell spheres settle, use a pipette to transfer the supernatant to a centrifuge tube, centrifuge at 1500 rpm for 20 minutes, and discard the supernatant. Single neural stem cells and small neural stem cell spheres were collected by centrifugation, and returned to the culture bottle, and fresh culture medium was added to culture. The disadvantage of this method is that it is time-consuming and laborious, and the centrifuged precipitation liquid contains long-term cultured cell fragments. Tissues containing these debris affect long-term culture of neural stem cells

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