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Application of C21 steroid compounds 1-8 of cynanchum paniculatum in preparation of anti-TSWV active products

A technology of steroids and compounds, applied in the field of biological pesticides, can solve problems such as

Inactive Publication Date: 2020-07-17
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current study found that the C obtained from Xu Changqing (Cynanchum paniculatum (Bunge) Ktag) 21 Steroidal compounds have anti-virus, insect-proof, bactericidal and other activities, but currently there is no C isolated from Xu Changqing. 21 Report on the application of steroidal compounds as preparations for preventing and controlling TSWV in the preparation of anti-TSWV drugs

Method used

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  • Application of C21 steroid compounds 1-8 of cynanchum paniculatum in preparation of anti-TSWV active products
  • Application of C21 steroid compounds 1-8 of cynanchum paniculatum in preparation of anti-TSWV active products
  • Application of C21 steroid compounds 1-8 of cynanchum paniculatum in preparation of anti-TSWV active products

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Experimental program
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Effect test

Embodiment 1

[0018] Compound extraction and separation:

[0019] The dried rhizomes of Xu Changqing were crushed and extracted three times by reflux with 95% methanol, two hours each time, concentrated to obtain the total extract, dissolved in water and extracted three times with dichloromethane. The dichloromethane layer was mixed with 60-80 mesh silica gel, and the silica gel (200-300 mesh column) was used for gradient elution, and the eluent was petroleum ether-acetone (1:0 to 1:2) to dichloromethane- Methanol (10:1 to 0:1). The same components were combined to obtain 8 components (1-8). Among them, petroleum ether / acetone 2:1 partial silica gel column chromatography, reverse RP-18 column, SephadexLH- 20 gel column, and more detailed separation by semi-preparative and preparative column HPLC, etc., to obtain compound 1-8.

[0020] The compound 1-8 structural formula that obtains with above-mentioned method is as follows:

[0021]

[0022] method

[0023] 1 Preparation of poison so...

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Abstract

The invention provides an application of C21 steroid compounds 1-8 separated from cynanchum paniculatum in preparation of anti-TSWV (tomato spotted wilt virus) active products. Tests prove that the C21 steroid compounds 1-8 in cynanchum paniculatum have anti-TSWV activity, and show different degrees of prevention effects on TSWV. Specifically, the invention also provides an application of the C21steroid compounds 1-8 of cynanchum paniculatum in preparation of a TSWV inhibitor. When the concentrations of the compound 4 and the compound 5 are 100 [mu]g / mL, the virus content is less than that ofningnanmycin, which indicates that the prevention effect on TSWV is superior to that of commercially available antiviral pesticide ningnanmycin.

Description

technical field [0001] The invention belongs to the field of biopesticides, in particular to C 21 Steroidal compounds 1-8 are used as preparations for the prevention and control of TSWV in the preparation of anti-TSWV active products. Background technique [0002] Tomato spotted wilt virus (TSWV) is a representative species of the genus Tospovirus in the family Bunyaviridae. Its host range is very wide, and many important economic crops (such as tobacco, pepper, Tomato) are susceptible to infection and cause serious economic losses, so it is listed as one of the top ten plant viruses in the world. At present, the prevention and control of viral diseases in production is mainly through insect control and disease prevention, but its transmission vectors are prone to produce resistance, and crops are prone to problems such as excessive pesticide residues. In addition, there is a lack of disease-resistant varieties, and there is still a lack of effective prevention and control ...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01P1/00
CPCA01N43/90
Inventor 李顺林龙琴宝玉杨宝嘉
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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