Preparation method of anti-tumor macrophages

Abstract

The invention discloses a preparation method of anti-tumor macrophages. The method comprises the following steps of obtaining primary bone marrow stem cells or separating mononuclear cells in peripheral blood for primary cell culture, adding a colony stimulating factor, and after 7 days, obtaining macrophages; obtaining in-vitro tumor cells for primary tumor cell culture, and obtaining primary tumor cells; performing radiotherapy irradiation on the obtained primary tumor cells and a culture solution, collecting supernate after radiotherapy, and obtaining a mixture of required microparticles and apoptotic tumor cell debris; centrifuging the obtained mixture to obtain tumor antigen-carrying microparticles; and treating the primary macrophages obtained in the step 1 with the obtained microparticles to obtain the anti-tumor macrophages. According to the preparation method of the anti-tumor macrophages, personalized treatment is realized, and the macrophages capable of aiming at different tumor antigens of patients is prepared; and the preparation process is simple and relatively easy in production.

Description

technical field [0001] The present invention relates to the field of anti-tumor, more particularly, relates to a preparation method of anti-tumor macrophage method. Background technique [0002] Macrophages are the key executors of downstream effects in the immune response. In the tumor microenvironment, macrophages account for more than 50%. More importantly, the proportion of macrophages is negatively correlated with the prognosis of tumors, which further illustrates the importance in tumors. Macrophages (Tumor Associated Macrophages, TAMs) in the infiltrating area of ​​tumor tissue promote the progression of tumors. Tumor cells combine with high expression of CD47 and SIRPα on the surface of macrophages, which directly leads to tumor cells not only living in harmony with tumor cells, but also It will promote the proliferation and growth of tumors by promoting the proliferation of blood vessels in tumors and inhibiting effector T cells. In the past, the application of ma...

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Problems solved by technology

[0004] In order to solve the above problems, the present invention provides a method for preparing anti-tumor macrophages. The anti-tumor macrophages exert anti-tumor effects by directly phagocytizing tumors and activating T cells, so as to solve the problem of poor efficacy of conventional chemotherapy, large side effects, and easy occurrence of Technical issues of drug resistance and enabling individualized treatment

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