Gelsolin inclusion and nano-crystallization method and suspension

A gelsolin and nanosuspension technology, which is applied in the field of protein inclusion, can solve the problem of rare inclusion of protein substances, and achieves the effects of good inclusion effect, stable properties and simple method.

Active Publication Date: 2020-09-25
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the substances commonly used for inclusion with cyclo...

Method used

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  • Gelsolin inclusion and nano-crystallization method and suspension

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Preparation of inclusion complexes

[0039] Weigh 10g of HP-β-CD, put it in a mortar, add 20ml of distilled water at 35°C, and grind for 15 minutes. Weigh 1g of gelsolin, dissolve it completely in 10ml of distilled water, slowly add it dropwise into the mortar with a constant flow pump, mix it with HP-β-CD, grind it while adding it, and continue grinding until the addition is completed. The total time is controlled at 4h. The mixture was placed in a dialysis bag, filtered and transferred to a freeze dryer, and freeze-dried to obtain a cyclodextrin / gelsolin inclusion compound.

[0040] 2. Detection of inclusion complex characterization

[0041]The formation of inclusion complexes was verified by UV spectroscopy. The ultraviolet spectrum of gelsolin HP-β-CD inclusion complex is different from that of pure gelsolin and the simple mixture of the two; pure gelsolin has an obvious absorption peak at 280nm, while HP-β-CD is at this wavelength There is almost no absorpti...

Embodiment 2

[0048] 1. Preparation of inclusion complexes

[0049] Weigh 8g of HP-β-CD, put it in a mortar, add 20ml of distilled water at 30°C, and grind for 15 minutes. Weigh 1g of gelsolin, dissolve it completely in 10ml of distilled water, slowly add it dropwise into the mortar with a constant flow pump, mix it with HP-β-CD, grind it while adding it, and continue grinding until the addition is completed. The total time is controlled at 4h. The mixture was placed in a dialysis bag, filtered and transferred to a freeze dryer, and freeze-dried to obtain a cyclodextrin / gelsolin inclusion compound.

[0050] 2. Detection of inclusion complex characterization

[0051] The formation of inclusion complexes was verified by UV spectroscopy. The ultraviolet spectrum of gelsolin HP-β-CD inclusion complex is different from that of pure gelsolin and the simple mixture of the two; pure gelsolin has an obvious absorption peak at 280nm, while HP-β-CD is at this wavelength There is almost no absorpti...

Embodiment 3

[0058] 1. Preparation of inclusion complexes

[0059] Weigh 9g of HP-β-CD, put it in a mortar, add 20ml of distilled water at 40°C, and grind for 15 minutes. Weigh 1g of gelsolin, dissolve it completely in 10ml of distilled water, slowly add it dropwise into the mortar with a constant flow pump, mix it with HP-β-CD, grind it while adding it, and continue grinding until the addition is completed. The total time is controlled at 4h. The mixture was placed in a dialysis bag, filtered and transferred to a freeze dryer, and freeze-dried to obtain a cyclodextrin / gelsolin inclusion compound.

[0060] 2. Detection of inclusion complex characterization

[0061] The formation of inclusion complexes was verified by UV spectroscopy. The ultraviolet spectrum of gelsolin HP-β-CD inclusion complex is different from that of pure gelsolin and the simple mixture of the two; pure gelsolin has an obvious absorption peak at 280nm, while HP-β-CD is at this wavelength There is almost no absorpti...

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Abstract

The invention belongs to the field of nanoparticle preparation in the biochemical technique, and particularly relates to a gelsolin inclusion and nano-crystallization method and a suspension. According to the implementation scheme, in parts by weight, under the normal temperature condition, gelsolin and hydroxypropyl-beta-cyclodextrin are added into a mortar in a proportion of 1:10, and a small amount of purified water is added for grinding for 4h; and then, a mixture of the gelsolin and hydroxypropyl-beta-cyclodextrin is filtered and freeze-dried to obtain a cyclodextrin/gelsolin inclusion complex. The inclusion complex is nano-crystallized to obtain the nanosuspension of a gelsolin-cyclodextrin inclusion complex. The method of the invention does not use an organic solvent or toxic cross-linking agent, has simple operation, a good inclusion effect and high repeatability, and is safe and reliable.

Description

technical field [0001] The invention relates to a method for preparing nanoparticles in the field of biotechnology, in particular to a protein inclusion method. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The most commonly used protein inclusion material is chitosan. Chitosan is the only alkaline polysaccharide that occurs naturally. Low toxicity, renewable, excellent biodegradability and biocompatibility. Its molecular chain is rich in hydroxyl and amino groups, which are easy to carry out chemical reactions. However, chitosan is insoluble in water and general organic solvents, but soluble in dilute inorganic acids and some organic acids. The defect that chitosan...

Claims

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Application Information

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IPC IPC(8): A61K9/10A61K47/69A61K38/17A61P29/00A61P17/02A61P35/00
CPCA61K9/10A61K38/1709A61K47/6951A61P17/02A61P29/00A61P35/00
Inventor 李玮范立嵩郄淑燕杭伟袁双虎雷晟暄马志祥孟祥伟孙海航
Owner SHANDONG UNIV
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