Multi-mode precisely-focused electrical impedance flow cytometry detection device and preparation method thereof

A flow cytometry and detection device technology, applied in the direction of material resistance, can solve the problems of inaccurate focusing position, clogging of sample particles, single detection mode, etc., achieving fast focusing speed, accurate ultrasonic focusing position, and realizing focusing speed. Effect

Inactive Publication Date: 2020-10-02
SOUTHEAST UNIV
View PDF10 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: The purpose of the present invention is to provide a multi-mode precise focusing electrical impedance flow cytometry

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multi-mode precisely-focused electrical impedance flow cytometry detection device and preparation method thereof
  • Multi-mode precisely-focused electrical impedance flow cytometry detection device and preparation method thereof
  • Multi-mode precisely-focused electrical impedance flow cytometry detection device and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] The present invention will be further described below in conjunction with the accompanying drawings.

[0022] Such as Figure 1-9 As shown, the multi-mode precise focusing electrical impedance flow cytometric detection device includes a non-conductive main body, and the main body is sequentially provided with a hydrodynamic focusing flow channel 13, an ultrasonic focusing flow channel 14, and an electrical impedance detection flow channel from the fluid inlet to the outlet direction. channel 15 and outlet flow channel 16, the hydrodynamic focusing flow channel 13 includes the sample flow channel 11 in the middle and the sheath liquid flow channel wrapped outside the sample flow channel, the sheath liquid flow inlet flow channel 12 and the hydrodynamic focusing flow channel 13 The included angle is 90°, the sample flow channel 11 is connected to the sample inlet 41, the sheath liquid flow channel is connected to the sheath liquid inlet 42 through the sheath liquid flow c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a multi-mode precisely-focused electrical impedance flow cytometry detection device and a preparation method thereof. The device comprises a non-conductive body, and a hydrodynamic focusing flow channel, an ultrasonic focusing flow channel, an electrical impedance detection flow channel and an outlet flow channel are sequentially arranged in the body in the direction from the fluid inlet to the fluid outlet. The hydrodynamic focusing flow channel comprises a sample flow channel in the middle and a sheath fluid flow channel wrapping the sample flow channel; the sample flow channel is communicated with the sample inlet, the sheath fluid flow channel is communicated with the sheath fluid inlet through the sheath fluid flow inlet flow channel, a piezoelectric element isarranged on the outer side of the ultrasonic focusing flow channel, electrical impedance detection electrodes are arranged at the two ends of the electrical impedance detection flow channel, and double-nozzle 3D printing is adopted in the preparation method. According to the invention, the problem of congestion of a sample particle blocking point is solved, the targets of high focusing speed andaccurate focusing position are realized, rapid, accurate and effective focusing is carried out on cells, and the difficulty of signal processing is reduced.

Description

technical field [0001] The invention relates to a microfluidic detection device, in particular to a multi-mode precise focusing electrical impedance flow cytometric detection device and a preparation method. Background technique [0002] Microfluidic devices are usually also called microfluidic chips, which are mainly used in chemical analysis, biomedicine, environmental monitoring and other fields. Combined with flow cytometry, it is often used to detect particles such as cells, genetic material, and proteins. During detection, the sample particles in the micro-channel should be stably focused at the center to improve detection accuracy. The methods of focusing mainly include methods such as hydrodynamic focusing and acoustic focusing, and the methods of detection mainly include methods such as electrical impedance detection, fluorescence detection, light scattering, and microscopic imaging. [0003] The hydrodynamic focusing method exerts a large focusing force on the ce...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N27/07
CPCG01N27/07
Inventor 杨雯皓韩煜
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap